Ochratoxin A (OTA) is a mycotoxin with main nephrotoxic activity contaminating several foodstuffs. In the present report, five soil samples collected from OTA-contaminated vineyards were screened to isolate microorganisms able biodegrade OTA. When cultivated in OTA-supplemented medium, OTA was converted OTα by 225 bacterial isolates. To reveal clonal relationships between isolates, molecular typing using an automated rep-PCR system carried out, thus showing presence of 27 different strains...

The performance of probiotic bacterial strains is influenced by the carrier food and its functional components which while buffering through gastro-intestinal tract, contribute to an efficient implantation cells regulate features. Particularly, plant-based matrices are eligible substrate for hosting delivering microbial populations because their richness in nutrients, fibers, vitamins, minerals dietary bioactive phytochemicals. available data indicate that intrinsic health-promoting...

Abstract The present study compares the pathogenicity on olive and oleander plants of three wild‐type strains Pseudomonas syringae subsp. savastanoi (ITM317 PBa230 from ITM519 oleander) phytohormone‐deficient mutants ITM519: ITM519‐41 (Iaa + /cytokinins‐), ITM519‐7 − /cytokinins ), ITM519‐6 Mutants not producing IAA (ITM519‐7and ITM519‐6) only induced necrosis inoculated tissues (ITM519‐,6) or swellings stems attributed to cytokinin production accompanied by (ITM519‐7). By contrast, Iaa...

The virulence of Pseudomonas syringae subsp. savastanoi, which causes hyperplastic symptoms (knots) on olive plants, is associated with secreted phytohormones. We identified a Tn5-induced mutant P. savastanoi that did not cause disease plants although it was still able to produce In addition, the failed elicit hypersensitive response in nonhost plant. Molecular characterization revealed single Tn5 insertion occurred within an open reading frame encoding protein 92% identical HrcC pv....

Results of a survey olive knot disease in central Italy 2002 and 2003 showed that Pantoea agglomerans was found associated with the pathogen Pseudomonas savastanoi pv. ( Ps. ) 70% knots examined. Pathogenicity tests which these two bacteria were co‐inoculated on stems 1‐year‐old plants at ratios 1:1, 1:100 100:1 growth P. apparently aided by presence an actively growing population . At same time, however, dominant inoculation site tended to depress , probably because competition for space...

To evaluate the positive influence of probiotic strain Lactobacillus paracasei LMGP22043 carried by artichokes into human gut with special reference to faecal bacterial balance, short-chain fatty acid concentrations and enzyme activities in a randomized, double-blind trial comparison probiotic-free (control).Twenty subjects were randomized two groups, which consumed daily 180 g artichoke product (probiotic or control) during 15-day study periods (periods 1 2) separated washout crossover...

Probiotic bacteria are generally available for consumers as concentrated preparations or incorporated in milk-based foods. Due to an increased interest of the market probiotic foods well meet a demand industry innovation, new kind food has been developed using table olives carrier. Green olives, produced according Spanish-style, obtained by fermentation which can be carried out spontaneous microflora, even if use starter cultures is desirable obtain more controlled process. In this regard,...

The aim of this study was the characterization and selection bacterial strains suitable for production a “yeast-free” bread. Leuconostoc citreum C2.27 Weissella confusa C5.7 were selected their leavening acidification capabilities individually used as starters in bread-making tests. Liquid type-II sourdoughs, singly inoculated with two strains, characterized employed bread-making, through set-up biotechnological protocol without use baker’s yeast agent. Aiming to verify ability dominate...

The most recent trend in research on probiotic bacteria aims at the exploitation of bioactive bacterial compounds that are responsible for health-promoting effects and suitable medical applications. Therefore, main purpose this study was to ascertain if immunomodulatory L. paracasei strains dendritic cells (DCs) were caused by metabolites released culture medium. For reason, grown two media generally used DCs, filtrates maturation DCs cytokine production evaluated. Moreover, reveal potential...

The genetic diversity of 71 Pseudomonas savastanoi pv. strains isolated from different host species and diverse geographical regions was determined by fluorescent amplified fragment length polymorphism (f‐AFLP) analysis. study carried out using three selective primer combinations. Strains P. syringae , phaseolicola glycinea tagetis amygdali were also included as outgroups. Based on cluster analysis f‐AFLP data, all showed a high degree similarity, grouping in forming taxon clearly separate...

Pseudomonas savastanoi pv. is the causal agent of Olive knot disease, relying on Type Three Secretion System (TTSS) for its pathogenicity. In this regard, nothing was known about two other pathovars belonging to species, nerii and fraxini, characterized by a different host range. Here we report organization entire TTSS cluster three pathovars, phylogenetic analysis including those bacteria P. syringae complex sequenced so far, highlighting evolution each operon (hrpC, hrpJ, hrpRS, hrpU...

This study was performed to ascertain the immunomodulatory effect of Lactobacillus paracasei strains. These strains were also genetically characterized.The differentiated by using fluorescent-amplified fragment length polymorphism technique, which led identification several molecular markers unique each strain. To determine properties, we evaluated on dendritic cell maturation, dextran uptake, ability induce proliferation allogenic T cells and cytokine secretion. The results indicated that...

Bacteria forming levan colonies and not producing fluorescent pigments have been isolated from olive knots the phylloplane in central Italy. By their pathogenicity to morphological, biochemical physiological features, they clearly belong Pseudomonas syringae subsp. savastanoi.