Human activities affect Earth's life support systems so profoundly as to threaten many of the ecological services that are essential society. To address this challenge, a new science agenda is needed integrates people with rest nature help chart more sustainable trajectory for relationship between society and biosphere. This paper describes Earth Stewardship, an initiative Ecological Society America provide scientific basis actively shaping trajectories social-ecological change enhance...

Despite substantial evidence that writing can be an effective tool to promote student learning and engagement, writing-to-learn (WTL) practices are still not widely implemented in science, technology, engineering, mathematics (STEM) disciplines, particularly at research universities. Two major deterrents progress the lack of a community science faculty committed undertaking applying necessary pedagogical research, absence conceptual framework systematically guide study designs integrate...

Developing critical-thinking and scientific reasoning skills are core learning objectives of science education, but little empirical evidence exists regarding the interrelationships between these constructs. Writing effectively fosters students' development constructs, it offers a unique window into studying how they relate. In this study undergraduate thesis writing in biology at two universities, we examine exhibited (assessed using Biology Thesis Assessment Protocol) relates to general...

tein B; SDS, sodium

The apolipoprotein B polypeptide of human serum low density lipoprotein exists (after reduction disulfide bonds) as a random coil with molecular weight 250,000 in concentrated solutions guanidine hydrochloride. With intact bonds, there is limited restraint on the conformation this denaturing solvent. In presence saturating amounts bound sodium dodecyl sulfate, dimeric and highly asymmetric. This work substantiates monomeric for reported by others (Smith, R., Dawson, J.R., Tanford, C. (1972)...

One of the best opportunities that undergraduates have to learn write like a scientist is thesis after participating in faculty-mentored undergraduate research. But developing writing skills doesn't happen automatically, and there are significant challenges associated with offering courses individualized mentoring. We present hybrid model which students structural support course plus personalized benefits working one-on-one faculty. To optimize these interactions, uses BioTAP, Biology Thesis...

Undergraduate theses and other capstone research projects are standard features of many science curricula, but participation has typically been limited to only the most advanced highly motivated students. With recent push engage more undergraduates in research, some faculty finding that their typical approach working with thesis writers is less effective, given wider sersity students, or inefficient, higher rates. In these situations, a formal process may be needed ensure all students...

The Mitsunobu reaction involves the "redox" condensation of an acidic pronucleophile with a primary or secondary alcohol promoted by reactive phosphonium salt derived from alkyl aryl phosphine and azo compound. synthetic utility is demonstrated wide range pronucleophiles that are tolerated including carboxylic acids, phenols, imides, hydroxamates, thiols, thioamides, fluorinated alcohols, oximes, pyridinium imidazolium salts, pyrimidine bases, β-ketoesters trimethylmethane tricarboxylate...

Abstract The COVID‐19 pandemic has created new challenges for instructors who seek high‐impact educational practices that can be facilitated online without creating excessive burdens with technology, grading, or enforcement of honor codes. These must also account the possibility some students may need to join courses asynchronously and have limited unreliable connectivity. Of American Association Colleges University's list 11 practices, writing‐intensive easiest science faculty adopt during...

The transport rate of an electrogenic ion pump, and therefore also the current generated by depends on potential difference (delta psi) between two sides membrane. This dependence arises from at least three sources: (i) charges carried across membrane transported ions; (ii) protein in binding sites that alternate exposure to (and electrical contact with) membrane; (iii) or dipoles move within domain as a result conformational changes linked cycle. Quantitative prediction these separate...

Cooperative interaction of L-alpha-didecanoyl phosphatidylcholine with the AI polypeptide from human serum high density lipoprotein results in a complex particle containing 2 molecules and 190 lipid. Binding occurs two orders magnitude above critical micelle concentration lipid requires simultaneous presence low levels single-chain amphiphiles. The present investigation previous studies this laboratory suggest that contains "pockets" capable interacting fixed hydrophobic volume amphiphilic...

The conventional alternating access model for Ca2+ transport by the sarcoplasmic reticulum pump is modified, partly on basis of proposed MacLennan-Green domain structure Ca2+-pump protein. present divides uptake state (E1) protein into three substates, differing in condition Ca2+-binding domain. an open cavity first substate and can bind only a single ion. A fast "jaw-closing" (or "hinge-bending") step then partially closes to generate second that has site. Occupation this site followed...

Abstract Programs designed to broaden participation in science are often deemed “successful” based on quantitative evidence such as student rates, retention, and persistence. These numbers alone only explain that a program met its goals; they seldom critically how , specifically, the achieved success. To address this gap, we studied students’ perspectives about experiences with Ecological Society of America's award‐winning education diversity mentoring program, Strategies for Ecology...

The sarcoplasmic reticulum Ca-pump protein can be solubilized in monomeric form nonionic detergents with full retention of ATPase activity. It is impossible to prove directly that coupling between ATP hydrolysis and Ca2+ transport maintained the soluble state, because separate compartments for uptake discharge are required demonstrate this, but here we provide strong indirect evidence fact persists, both forward reverse directions normal pump cycle. Demonstration direction makes use...

A pervasive notion in the literature is that complex concept maps reflect greater knowledge and/or more expert-like thinking than less maps. We show used to structure scientific writing and clarify reasoning do not adhere this notion. In an undergraduate course for thesis writers, students use instead of traditional outlines define boundaries scope their research construct argument significance research. Students generate at beginning semester, revise after peer review, once end semester....

Professional societies can, and should, recruit retain young scientists by providing a welcoming inclusive intellectual home. SEEDS (Strategies for Ecology Education, Diversity Sustainability), the flagship education program of Ecological Society America, is designed to broaden participation in ecology through mentoring, field trips, leadership development, research fellowships. Nationally, fewer than 40% college students who intended pursue career science, technology, math, or engineering...

The Department of Economics at Duke University has endeavored to increase participation in undergraduate honors thesis research while ensuring a high-quality learning experience. Given the faculty-to-student ratio department (approximately 1:16), increasing required creation stable, replicable framework for mentoring students through research. aimed make experience more consistent and interactive so that also learned from each other group setting. Here, authors assess relationship between...

If a ligand binds with unequal affinity to two distinct states of protein, then the equilibrium between becomes function concentration ligand. A necessary consequence is that must also affect forward and/or reverse rate constants for transition states. For an enzyme or transport protein such as slow step in catalytic cycle, overall concentration. These conclusions are independent whether not direct participant reaction. it participant, kinetic effect arising from principle linked functions...

This paper examines the North American sea urchin fishery, with a particular focus on Japanese wholesale market. After brief history and discussion of biology, methods harvesting are discussed, followed by an overview processing, handling, transportation links. An econometric model price determination in Tokyo Central Wholesale Market is developed estimated. The reveals important mechanisms governing prices imported product; particular, interconnections between domestic markets, role...

Contrary to common belief, theoretical analysis does not predict any necessary relationship between cooperativity in the equilibrium binding of an ion multiple sites on a protein and kinetic activation reaction for which such is prerequisite. The sarcoplasmic reticulum Ca pump protein, example, has two high-affinity Ca2+, here considered be nearly identical independent. Equilibrium these can highly cooperative spite site-independence, as demonstrated by well-known allosteric mechanism based...

We have studied the structure of totally delipidated polypeptide (apolipoprotein B [apo B]) present in low-density serum lipoprotein detergent (n-dodecyl octaethyleneglycol monoether) solution by electron microscopy. The protein-detergent complex appears as a rod-shaped particle, 75-80 nm long and 4.5-5.5 wide. volume this particle is consistent with previously published composition reported Watt Reynolds (1980, Biochemistry 19:1593-1598) two copies apo five to six equivalent micelles...