A5041793009- Autophagy in Disease and Therapy
- Viral Infectious Diseases and Gene Expression in Insects
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Microbial Metabolic Engineering and Bioproduction
- Gene Regulatory Network Analysis
- Endoplasmic Reticulum Stress and Disease
- Bioinformatics and Genomic Networks
- Retinal Development and Disorders
- Computational Drug Discovery Methods
- Pancreatic function and diabetes
- Advanced Fluorescence Microscopy Techniques
Telethon Institute Of Genetics And Medicine
2011-2020
University of Naples Federico II
2013
The human retina is a specialized tissue involved in light stimulus transduction. Despite its unique biology, an accurate reference transcriptome still missing. Here, we performed gene expression analysis (RNA-seq) of 50 retinal samples from non-visually impaired post-mortem donors. We identified novel transcripts with high confidence (Observed Transcriptome (ObsT)) and quantified the level known (Reference (RefT)). ObsT included 77 623 (23 960 genes) covering 137 Mb (35 new transcribed...
Abstract Motivation: Identification of differential expressed genes has led to countless new discoveries. However, differentially are only a proxy for finding dysregulated pathways. The problem is identify how the network regulatory and physical interactions rewires in different conditions or disease. Results: We developed procedure named DINA (DIfferential Network Analysis), which able set genes, whose co-regulation condition-specific, starting from collection condition-specific gene...
In response to starvation, cells tune protein synthesis promote autophagy, thus ensuring nutrient recycling and survival.
Understanding the relationship between topology and dynamics of transcriptional regulatory networks in mammalian cells is essential to elucidate biology complex signaling pathways. Here, we characterised, via a synthetic approach, positive feedback loop (PFL) by generating clonal population (CHO) carrying stable integration construct. The PFL network consists Tetracycline-controlled transactivator (tTA), whose expression regulated tTA responsive promoter (CMV-TET), thus giving rise feedback....