A5042728362- Alzheimer's disease research and treatments
- Neuroscience and Neural Engineering
- Pluripotent Stem Cells Research
- Renal and related cancers
- Neurogenesis and neuroplasticity mechanisms
- Medicinal Plants and Neuroprotection
- Axon Guidance and Neuronal Signaling
- CRISPR and Genetic Engineering
- Nerve injury and regeneration
- Ion channel regulation and function
- Cholesterol and Lipid Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Genomics and Rare Diseases
- Pharmaceutical studies and practices
- Advanced Memory and Neural Computing
- Wnt/β-catenin signaling in development and cancer
- Nanocluster Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Retinoids in leukemia and cellular processes
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Lipid Membrane Structure and Behavior
Philipps University of Marburg
2016-2023
Heinrich Heine University Düsseldorf
2012-2019
Institut des Neurosciences Cellulaires et Intégratives
2007-2009
Centre National de la Recherche Scientifique
2007-2009
Neurons have a high demand for cholesterol to develop and maintain membrane-rich structures like axons, dendrites synapses, but it remains unclear, whether they can satisfy their need by costly de novo synthesis. To address this, we compared synthesis in serum-free cultures of highly purified CNS neurons glial cells from postnatal rats. We observed marked cell-specific differences: Compared with cells, showed different profiles biosynthetic enzymes, post-squalene precursors metabolites,...
Abstract Human induced pluripotent stem cell (iPSC)-derived neurons have been proposed to be a highly valuable cellular model for studying the pathomechanisms of Alzheimer's disease (AD). Studies employing patient-specific human iPSCs as models familial and sporadic forms AD described elevated levels AD-related amyloid- β (A ). However, none present iPSC studies could recapitulate synaptotoxic actions A , which are crucial early events in cascade that eventually leads vast brain...
Abstract Human astrocytes differ dramatically in cell morphology and gene expression from murine astrocytes. The latter are well known to be of major importance the formation neuronal networks by promoting synapse maturation. However, whether human astrocyte lineage cells have a similar role network has not been firmly established. Here, we investigated impact on functional maturation neural that were derived induced pluripotent stem (hiPSCs). Initial vitro differentiation hiPSC‐derived...
The aetiology of Alzheimer's disease is thought to include functional impairment synapses and synapse loss as crucial pathological events leading cognitive dysfunction memory loss. Oligomeric amyloid-β peptides are well known induce damage, destabilization brain synapses. However, the complex molecular mechanisms action resulting ultimately in elimination incompletely understood, thus limiting knowledge potential therapeutic targets. Under physiological conditions, long-term stability...
Abstract There is increasing evidence that different phases of brain development depend on neuron–glia interactions including postnatal key events like synaptogenesis. To address how glial cells influence synapse development, we analyzed whether and glia‐derived factors affect gene expression in primary cultures immunoisolated rat retinal ganglion (RGCs) by oligonucleotide microarrays. Our results show the transcript pattern matched developmental stage characteristic properties RGCs vitro ....
Synapse elimination and pruning of axon collaterals are crucial developmental events in the refinement neuronal circuits. While a control synapse formation by adhesion molecules is well established, involvement loss poorly characterized. To investigate consequences mis-match expression homophilic synaptic molecule, we analysed an asymmetric, exclusively postsynaptic N-cadherin. This was induced transfecting individual neurons cultures N-cadherin knockout mouse with vector. 2 days after...
Brain-derived neurotrophic factor (BDNF) is known to be a crucial regulator of neuronal survival and synaptic plasticity in the mammalian brain. Furthermore, BDNF positively influences differentiation embryonic neural precursors as well stem cells from adult neurogenic niches. To study impact cell-released on (ESCs), which represent an attractive source for cell transplantation studies, we have generated BDNF-GFP overexpressing mouse ESC clones by knock-in technology. After vitro, observed...
Because of high exposure to systemic noxae, vascular endothelial cells (EC) have ensure distinct damage defense and regenerative mechanisms guarantee health. For meaningful toxicological drug assessments employing embryonic stem cell (ESC)-based in vitro models, functional competence differentiated progeny detailed knowledge regarding are essential. Here, mouse ESCs (mESC) were into functionally competent (EC smooth muscle [SMC]). mESC, EC, SMC comparatively analyzed DNA repair response...
Presenilin-1 (PSEN1) is the catalytic subunit of intramembrane protease γ-secretase and undergoes endoproteolysis during its maturation. Heterozygous mutations in PSEN1 gene cause early-onset familial Alzheimer's disease (eFAD) increase proportion longer aggregation-prone amyloid-β peptides (Aβ42 and/or Aβ43). Previous studies had suggested that mutants might act a dominant-negative fashion by functional impediment wild-type PSEN1, but exact mechanism which promote pathogenic Aβ production...
Brain-derived neurotrophic factor (BDNF) is known to be a crucial regulator of neuronal survival and synaptic plasticity in the mammalian brain. Furthermore, BDNF positively influences differentiation embryonic neural precursors, as well that stem cells from adult neurogenic niches. To study impact cell-released on (ESCs), which represent an attractive source for cell transplantation studies, we have generated mouse ESC clones overexpressing BDNF-GFP by use knock-in technology. After vitro,...
In this study, lymphoblastoid cells derived from a 83-year old individual with 15year history of progressive presenile dementia, were used to generate iPS cells, employing episomal plasmids expressing OCT4, SOX2, LIN28, L-MYC and p53 shRNA. The was homozygous for the APOE4 allele. resulting had normal karyotype, retained APOE4/4 genotype, expressed pluripotency markers, free genomically integrated plasmids, could be differentiated into cell type representatives three germ layers in vitro.
In this study, lymphoblastoid cells derived from a 79-year old individual with history of progressive presenile dementia, were used to generate iPS cells, employing episomal plasmids expressing OCT4, SOX2, KLF4, LIN28, L-MYC and p53 shRNA. The was homozygous for the APOE4 allele. resulting had normal karyotype, retained APOE4/4 genotype, expressed pluripotency markers, free genomically integrated plasmids, could be differentiated into cell type representatives three germ layers in vitro.