A5024443384- Herpesvirus Infections and Treatments
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- interferon and immune responses
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
- Veterinary medicine and infectious diseases
- Antimicrobial Peptides and Activities
- Clostridium difficile and Clostridium perfringens research
- Immunotherapy and Immune Responses
- Animal Disease Management and Epidemiology
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Vaccine Coverage and Hesitancy
- Pharmacological Effects of Natural Compounds
- Aquaculture disease management and microbiota
- Immune responses and vaccinations
- Vector-borne infectious diseases
- RNA Interference and Gene Delivery
- Viral-associated cancers and disorders
- Viral Infections and Immunology Research
- Poxvirus research and outbreaks
- Virus-based gene therapy research
University of Saskatchewan
2024
University of Guelph
2017-2024
Hokkaido University
2012-2016
Gamma delta (γδ) T cells play a significant role in the prevention of viral infection and tumor surveillance mammals. Although involvement γδ Marek’s disease virus (MDV) has been suggested, their detailed contribution to immunity against MDV or progression (MD) remains unknown. In current study, cell receptor (TCR)γδ-activated peripheral blood mononuclear (PBMCs) were infused into recipient chickens effects examined context formation by MDV. We demonstrated that adoptive transfer...
In the past, we demonstrated that oligodeoxynucleotides containing CpG motifs (CpG-ODN) mimicking bacterial DNA, stimulate innate immune system of neonatal broiler chickens and protect them against Escherichia coli Salmonella Typhimurium (S. Typhimurium) septicemia. The first line defense mechanism is formed by heterophils plays a critical protective role septicemia in avian species. Therefore, objectives this study were 1) to explore kinetics CpG-ODN mediated antibacterial mechanisms...
Abstract Oligodeoxynucleotides containing CpG motifs (CpG-ODN) can promote antimicrobial immunity in chickens by enriching immune compartments and activating cells. Innate memory, or trained immunity, has been demonstrated humans mice, featuring the absence of specificity to initial stimulus subsequently cross-protection against pathogens. We hypothesize that CpG-ODN induce chickens. delivered single multiple administrations birds mitochondrial oxidative phosphorylation (OXPHOS) glycolysis...
Marek's disease (MD) vaccines reduce the incidence of MD but cannot control virus shedding. To develop new vaccines, it is essential to elucidate mechanisms immunity (MDV) infection. In this regard, gamma delta (γδ) T cells may play a significant role in prevention viral spread and tumor surveillance. Here we demonstrated that MDV vaccination induced interferon (IFN)-γ+CD8α+ γδ transforming growth factor (TGF)-β+ lungs. from MDV-infected chickens exhibited cytotoxic activity. Importantly,...
Abstract Background An immunoinhibitory receptor, programmed death-1 (PD-1), and its ligand, death-ligand 1 (PD-L1), are involved in immune evasion mechanisms for several pathogens causing chronic infections neoplastic diseases. However, little has been reported the functions of these molecules chickens. Thus, this study, their expressions roles were analyzed chickens infected with Marek’s disease virus (MDV), which induces immunosuppression Results A chicken T cell line, Lee1,...
Infection with pathogenic viruses is often sensed by innate receptors such as Toll-Like Receptors (TLRs) which stimulate type I and III interferons (IFNs) responses, to generate an antiviral state within many cell types. To counteract these systems, viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), encode non-structural proteins (NSPs) that mediate immune evasion. Using overexpression system in A549 cells, we demonstrated a significant increase (p ≤ 0.0001)...
Transmission of H9N2 avian influenza virus (AIV) can occur in poultry by direct or indirect contact with infected individuals, aerosols, large droplets and fomites. The current study investigated the potential AIV transmission chickens via a fecal route. was monitored exposing naïve to material from AIV-infected (model A) experimentally spiked feces B). control received AIV. Results revealed that could persist for up 60-84 h post-exposure (PE). titers were higher at basic neutral pH. A...
The host response to pathogenic microbes can lead expression of interleukin (IL)-17, which has antimicrobial and anti-viral activity. However, relatively little is known about the basic biological role chicken IL-17A against avian viruses, particularly Marek’s disease virus (MDV). We demonstrate that, following MDV infection, upregulation mRNA an increase in frequency IL-17A+ T cells spleen occur compared control chickens. To elaborate on chIL-17A MD, full-length coding sequence was cloned...
(CP)-induced necrotic enteritis (NE) is an economically important disease in the broiler chicken industry. The incidence of NE common 3-to-6-wk-old chickens, once maternal antibodies start declining. Developing effective vaccination strategy against NE, preferably delivering a single dose vaccine at hatch to protect chickens without booster vaccine, enormous challenge. objective this study was induce mucosal immunity intestines by intrapulmonary (IPL) delivery live CP hatch, exploiting...
Vaccines against Marek's disease can protect chickens clinical disease; however, infected continue to propagate the virus (MDV) in feather follicles and shed into environment. Therefore, present study investigated if MDV could induce an immunoregulatory microenvironment feathers of whether vaccines overcome immune evasive mechanisms MDV. The results showed abundance CD4+CD25+ CD4+ transforming growth factor-beta (TGF-β)+ T regulatory cells MDV-infected at 21 days post-infection. In contrast,...
Gallid herpesvirus 2 (GaHV-2) causes malignant lymphomas in chickens (Marek's disease, MD).Although MD is controlled through vaccination efforts, field isolates of GaHV-2 have increased virulence worldwide and even cause vaccinated chickens.GaHV-2 strains are classified into four categories (mild, virulent, very virulent +) based on the exhibited experimental infection unvaccinated or MD-vaccinated susceptible chickens.Although cases sporadically reported Japan, recent Japan not been...
Marek’s disease (MD), caused by the virus, is a lymphoproliferative in chickens that can be controlled vaccination. However, current vaccines limit tumor growth and death but not virus replication transmission. The present study aimed to evaluate host responses following intramuscular injection of an mRNA vaccine encoding gB pp38 proteins MDV within first 36 h. was injected low high doses using prime prime-boost strategies. expression type I II interferons (IFNs), panel interferon-stimulated...