A5055031654- Genetic and phenotypic traits in livestock
- Microbial infections and disease research
- Reproductive Physiology in Livestock
- Ruminant Nutrition and Digestive Physiology
- Milk Quality and Mastitis in Dairy Cows
- T-cell and B-cell Immunology
- Regulation of Appetite and Obesity
- Animal Disease Management and Epidemiology
- Mycobacterium research and diagnosis
- Effects of Environmental Stressors on Livestock
- Cystic Fibrosis Research Advances
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- Immune Response and Inflammation
- Reproductive System and Pregnancy
- Molecular Biology Techniques and Applications
- Neonatal Respiratory Health Research
- Herpesvirus Infections and Treatments
- RNA modifications and cancer
- Vector-Borne Animal Diseases
- Animal health and immunology
- Aquatic Invertebrate Ecology and Behavior
- Neurological and metabolic disorders
- Autoimmune Neurological Disorders and Treatments
- Neurological Complications and Syndromes
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria
2012-2024
University of Lausanne
2022-2023
Cereal Research Centre
2023
Centro di Ricerca per l’Orticoltura
2017
Animal Production Research Centre
2012
Sapienza University of Rome
2002-2010
Istituto Sperimentale per la Zootecnia
1993-2007
Regione Lazio
2002-2004
The dynamic changes and structural patterns of DNA methylation genes without CpG islands are poorly characterized. relevance to the non-CpG equilibrium in transcriptional repression is unknown. In this work, we analyzed pattern 5'-flanking myogenin gene, a positive regulator muscle differentiation with no island low density, both C2C12 satellite cells embryonic muscle. Embryonic brain was studied as non-expressing tissue. High levels were observed experimental conditions. Both rapidly...
Heat stress negatively affects health, welfare, and livestock productivity by impairing immune function, increasing disease incidence. In recent years, there has been interest in understanding the system of water buffalo due to growing economic impact this species for high quality nutritional value milk. While are common responses across bovine species, also some species-specific variations physiological heat stress, mainly attributed differences metabolism dissipation efficiency. At...
The present study aimed to evaluate two commercial infectious bovine rhinotracheitis (IBR) marker vaccines against Bubaline alphaherpesvirus 1 (BuAHV-1) in water buffalo (Bubalus bubalis) . Thirteen buffaloes seronegative Bovine (BoAHV-1) and BuAHV-1 were selected divided into three groups (VAX-1, VAX-2, CNT). VAX-1 received an IBR (gE-/tk-) live vaccine; VAX-2 (gE-) inactivated CNT represented the controls. Two injections of 2 mL each administered 21 days apart. On 55 post-vaccination...
Brucellosis is a zoonotic disease, with an important economic impact on the livestock industry and public health worldwide. Both Brucella abortus melitensis can infect Mediterranean Buffalo ( Bubalus bubalis ), leading to infertility abortion. In ruminants, standard diagnostic approach involves two serological tests, Rose Bengal Test Complement Fixation Test, applied in parallel, though their specificity requires improvement. Cytokines play crucial role coordinating immune responses through...
Abstract Ruminal bacterial counts and in vivo digestibility were determined on four Mediterranean buffalo bulls Friesian bulls, all fistulated at the rumen, given maintenance level (50 g/kg M0·75 per day of dry matter) different diets with same crude protein content (N ✕ 6·25 = 140 forage: concentrate ratios as follows: diet D12·5 0·875: 0·125; D25·0 0·75: 0·25; D37·5 0·625: 0·375; D50·0 0·5: 0·5. All animals received during consecutive periods a Latin-square design. Buffaloes had higher...
To date, only a few SNP in the leptin receptor gene have been detected and analyzed while has extensively characterized several polymorphisms were associated to functional productive traits cattle. In this work, for first time, bovine was fully by sequencing whole coding region part of 5’ flanking region. A group 95 Holstein cows genotyped order search at both LEPR LEP genes, associate them with milk morphological traits. Nine novel one detected. Four showed significant effect on or more...
Recent studies have explored the seropositivity of Bovine alphaherpesvirus 1 (BoHV-1) in water buffaloes, suggesting urgency for developing strategies to eradicate virus involving both cattle and buffaloes. However, Europe, glycoprotein E (gE) deleted marker vaccines against BoHV-1 are commercially available only industry. This study, first time, evaluated safety efficacy a commercial inactivated gE-deleted vaccine buffalo. Five animals devoid BoHV-1-neutralizing antibodies were vaccinated...
Abstract Water buffalo ( Bubalus bubalis ) has a prominent position in the livestock industry worldwide but still suffers from limited knowledge on mechanisms regulating immune against infections, including brucellosis (BRC), one of most significant neglected zoonotic diseases livestock. Seventy‐three were recruited for study. Thirty‐five naturally infected with Brucella spp . The aims study to (i) verify cross‐reactivity 16 monoclonal antibodies (mAbs) developed human, bovine, and ovine...
Tuberculosis has a negative economic impact on buffalo farming, and it poses potential threat to human health. Interferon-gamma (IFN-γ) plays central role in protection against mycobacterial diseases, illustrating the importance of T-cell mediated immune responses tuberculosis infection. Recently, expression Caspase-3, critical executor apoptosis, M. tuberculosis-specific IFN-γ+CD4+ T cells was used as new marker distinguish active from latent infection humans. The aims this work were...
The lengths of the dinucleotide (TG)m and mononucleotide Tn repeats, both located at intron 8/exon 9 splice acceptor site cystic fibrosis transmembrane conductance regulator (CFTR) gene whose mutations cause (CF), have been shown to influence skipping exon in CFTR mRNA. This 9-skipped mRNA encodes a nonfunctional protein is associated with various clinical manifestations CF. As result growing interest these several assessment methods developed, most which are, however, cumbersome,...
The objective of this study was to analyze interferon-stimulated genes (ISGs) and interferon tau (IFNt) gene expression in peripheral blood leukocytes during the peri-implantation period until 40 days pregnancy buffalo cows. Relationships were also examined between ISGs IFNt pregnancy-associated glycoproteins (PAGs) plasma concentration. Buffalo cows synchronized artificially inseminated (d 0). Blood samples collected on 0, 18, 28 after artificial insemination (AI) for mononuclear cells...
Bubaline alphaherpesvirus-1 (BuAHV-1) and Bovine (BoAHV-1) are respiratory viruses that can cause an infection known as “Infectious Rhinotracheitis” (IBR) in both water buffalo bovine species. As the main disease control strategy, vaccination protect animals from clinical through development of specific humoral cell-mediated immune responses. In present study, time-related circulatory kinetics hematological profile bubaline monocyte subsets have been investigated vaccinated calves after...
Flow cytometry is a powerful technology used in many fields of cell biology. It also as routine method to count somatic cells milk and characterize bovine leukocytes. In this study, we flow simultaneously assess the viability, percentage single subsets leukocytes quantify expression CD11b, an immunological marker activation status. Immunological markers were then related with on farm recorded parameters electrical conductivity (MEC) average rate (MFR). Composite samples collected from 43...
Abstract The aim of the research reported in this paper was to evaluate plasma concentrations energy, oxidative and inflammatory biomarkers Simmental (sire) × Holstein (dam) crossbred cows, comparison with two parental breeds during peripartal early lactation periods estimate effects heterosis for these traits. Thirty-three animals, managed under same conditions, 8 (SI), 9 (HO) 16 (CR) cows were enrolled study. Glucose, non-esterified fatty acids (NEFA), β-hydroxybutyrate (BHB), total...
Abstract Several studies have reported the high bioindication capacity of Isopoda (Crustacea, Oniscidea), which is related to their important ability accumulate contaminants, usefulness in soil ecotoxicology and activities. Any change isopod population, diversity life cycle can indicate relevant pollution levels. The analysis target tissues, such as hepatopancreas, another emerging approach (from a cytologic/histological level) detect contaminant accumulation from different sources. In this...
The study aims to evaluate the potential use of mycobacterial ESAT6 and CFP10 antigens, Early Secretory Proteins (ESP) in Skin Test used for bovine tuberculosis (TB) diagnosis Water Buffalo.
 A pilot was performed on 21 buffaloes from a TB outbreak 11 TB-free herd. Three concentrations ESAT6-CFP10 (10, 20, 30 mg) two ESP (50 100 µg) were inoculated Test, along with PPDB, PPDA, PBS as negative control. thickness measured calipers before test every 24 hours 4 days. Then, specificity field...
The results of two different protocols neonatal cystic fibrosis (CF) screening in the Lazio region Italy are reported. first study, conducted from 1992 to 2000 on about 200,000 newborns, consisted an immunoreactive trypsin (IRT) protocol without mutation analysis transmembrane conductance regulator (CFTR) gene, referred as IRT/IRT protocol. Approximately 5% newborns with a positive IRT test were also at second test; approximately 57% high level subsequently found be affected by CF. In...
In this study, the effect of polymorphisms in leptin gene on hematological variables periparturient dairy cows was investigated. The profile 67 Holstein assessed for 6 wk around calving. DNA genotyped at polymorphic loci within gene, and 7 haplotypes were reconstructed. Significant haplotype substitution effects found, 1, total white blood cell count 2 calving (+0.70 10(3)/μL, P = 0.05; +1.38 0.0001); neutrophil first week after (+0.94 0.001); lymphocyte during 3 before (+0.32 +0.27 0.03;...