A5006790800- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Dendrimers and Hyperbranched Polymers
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- Curcumin's Biomedical Applications
- DNA and Nucleic Acid Chemistry
- Phytochemicals and Antioxidant Activities
- Advanced Drug Delivery Systems
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Lipid Membrane Structure and Behavior
- Viral Infectious Diseases and Gene Expression in Insects
- Natural product bioactivities and synthesis
- Advanced Glycation End Products research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiac Ischemia and Reperfusion
- Proteoglycans and glycosaminoglycans research
- Electrospun Nanofibers in Biomedical Applications
- Wound Healing and Treatments
- Plant biochemistry and biosynthesis
Konkuk University
2015-2024
Government of the Republic of Korea
2015-2019
Konkuk University Medical Center
2012-2019
Gwangju University
2015-2019
Korea Advanced Institute of Science and Technology
2002-2013
Korea Institute of Science and Technology
2013
Korea Research Institute of Bioscience and Biotechnology
2011-2013
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2012
University of Washington
2010-2011
University of Michigan–Ann Arbor
2010
Abstract The surface of bovine serum‐derived exosomes (EXOs) are modified with α‐ d ‐mannose for facile interaction mannose receptors on dendritic cells (DCs) and efficient delivery immune stimulators to the DCs. EXOs is polyethylene glycol (PEG) without particle aggregation (≈50 nm) via incorporation 1,2‐distearoyl‐sn‐glycero‐3‐phosphoethanolamine (DSPE) into lipid layer EXO, compared chemical conjugation by N ‐hydroxysuccinimide activated PEG (NHS‐PEG). modification onto exosomal...
Cell specific gene silencing effects of antisense oligodeoxynucleotide (AS-ODN), synthetic small interfering RNA (siRNA-S), and siRNA expressing plasmid (siRNA-P) were comparatively evaluated. Poly(ethylenimine) (PEI) PEI−graft-poly(ethylene glycol)−folate (PEI−PEG−FOL) conjugate used to form nanosized polyelectrolyte complexes with the above three nucleic acids coding for inhibition green fluorescent protein (GFP) expression. The acid formulated either PEI or PEI−PEG−FOL had comparable...
Xanthorrhizol, a natural sesquiterpenoid isolated from the rhizome of Curcuma xanthorrhiza Roxb (Zingiberaceae), has antibacterial activities and protective effects against cisplatin-induced hepatotoxicity. In this study, we investigated xanthorrhizol as an antioxidant or antiinflammatory agent using neuronal microglial cells. Xanthorrhizol had potent neuroprotective on glutamate-induced neurotoxicity reactive oxygen species (ROS) generation in murine hippocampal HT22 cell line. Also,...
A small interfering RNA (siRNA) nanovector with dual targeting specificity and therapeutic effect is developed for targeted cancer imaging therapy. The composed of an iron oxide magnetic nanoparticle core coated three different functional molecules: polyethyleneimine (PEI), siRNA, chlorotoxin (CTX). primary amine group PEI blocked citraconic anhydride that removable at acidic conditions, not only to increase its biocompatibility physiological conditions but also elicit a pH-sensitive...
Pluronic mimicking poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer having multiple hydroxyl groups in the PPO middle segment (core-functionalized Pluronic: CF-PLU) was synthesized for conjugation of doxorubicin (DOX). DOX conjugated on CF-PLU via an acid-labile hydrazone linkage (CF-PLU-DOX). In aqueous solution, CF-PLU-DOX copolymers self-assembled to form a core/shell-type micelle structure consisting hydrophobic DOX-conjugated core and...
Quantum dot (QD) and adenovirus (ADV) nanoparticles were surface-modified with graft copolymers that exhibited a charge reversal behavior under acidic condition. Poly(L-lysine) (PLL) was grafted multiple biotin-PEG chains (biotin-PEG-PLL copolymer), the remaining primary amine groups in PLL backbone postmodified using citraconic anhydride, pH-sensitive blocker, to generate carboxylate groups. The surfaces of streptavidin-conjugated QDs modified citraconylated biotin-PEG-PLL copolymer,...
Polyethylene glycol (PEG)-immobilized quantum dot (QD) nanoparticles, which could be specifically dePEGylated in response to the presence of matrix metalloprotease-2 (MMP-2) enzyme, were prepared. The degree PEGylation (MW 3400) on surface 12 nm streptavidin-coated QDs was stoichiometrically controlled by varying feed amount a biotin-substrate-PEG conjugate, where substrate contained an MMP-2 cleavable peptide sequence. A biotin-cell penetrating (CPP) conjugate also immobilized onto...
Indocyanine green (ICG) encapsulated hyaluronic acid (HA) nanogels were first studied for highly selective detection of specific cancers and lymph nodes via hyaluronidase sensitive switch-on near infrared fluorescence as a long-lasting stimuli-responsive imaging probe.
Mutation in KIF1B, a kinesin superfamily motor protein, causes peripheral neuropathy known as Charcot–Marie–Tooth disease type 2A (CMT2A). Little is known, however, about how defective KIF1B gene leads to CMT2A. Here we report that KIF1Bα, one of the two splice variants directly interacts through its C-terminal postsynaptic density-95 (PSD-95)/discs large/zona occludens (PDZ) domain-binding motif with PDZ proteins including PSD-95/synapse-associated protein-90 (SAP90), SAP97, and synaptic...
Abstract A novel self‐crosslinked and reducible peptide was synthesized for stable formation of nanoscale complexes with an siRNA‐PEG conjugate to enhance transfection efficiency in serum containing condition without compromising cytotoxicity. fusogenic peptide, KALA, two cysteine residues at both terminal ends crosslinked via disulfide linkages under mild DMSO oxidation condition. The KALA (cl‐KALA) used form nano‐complexes green fluorescent protein (GFP) siRNA. Size morphology various...
For therapeutic applications of small interfering RNA (siRNA), serum stability, enhanced cellular uptake, and facile endosome escape are key issues for designing carriers. In this study, green fluorescent protein (GFP) siRNA was conjugated to a six-arm polyethylene glycol (PEG) derivative via reducible disulfide linkage (6PEG-siRNA). The 6PEG-siRNA conjugate also functionalized with cell penetrating peptide, Hph1 enhance its uptake property (6PEG-siRNA-Hph1). 6PEG-siRNA-Hph1...
Because of RNA's ability to encode structure and functional information, researchers have fabricated diverse geometric structures from this polymer at the micro- nanoscale. With their tunable structures, rigidity, biocompatibility, novel two-dimensional three-dimensional RNA can serve as a fundamental platform for biomedical applications, including engineered tissues, biosensors, drug delivery vehicles. The discovery potential small-interfering (siRNA) has underscored applications RNA-based...