House fly larvae provide a prolific and sustainable source of proteins used in poultry fish feed. Wheat bran is superior diet for house has been widely investigated to exploit its potential the food feed area. Using Illumina MiSeq 16S rDNA sequencing, this study gut microbiota feeding on wheat bacterial community bran. The communities were dominated by phyla Proteobacteria Firmicutes. Enterobacteriaceae Providencia predominant bacteria at family genus levels, respectively. Some...

Exploring the pathogenicity of a new fungus strain to non-target host pests can provide essential information on large scale for potential application in pest control. In this study, we tested Beauveria bassiana PfBb important agricultural Spodoptera frugiperda (Lepidoptera: Noctuidae) by determining relative activities protective enzymes and detoxifying different larval instars. Our results show that B. could infect all six instars S. frugiperda, its virulence larvae gradually increased...

In the control of agricultural and forestry pests, excessive reliance on chemical pesticides has led to increasingly severe issues, such as toxic residues heightened pest resistance. The effective use biological become a major focus in management. Parasitoid wasps, critical natural enemy are widely distributed, diverse species, play an essential role control. Copidosomopsis nacoleiae, recently discovered polyembryonic endoparasitoid wasp, parasitizes Diaphania angustalis; yet, its...

Beauveria bassiana PfBb is a new strain with high host specificity to the target pest Phauda flammans. We conducted series of experiments optimize biphasic fermentation system B. by screening medium compositions and environmental conditions in both liquid solid fermentations. In fermentation, glucose yeast extract C:N ratio 17:1 were optimal carbon nitrogen sources, respectively, for mycelium growth blastospore production, an inoculation concentration 1 × 108/mL inoculum content 50 mL...

Cities in plain areas have small slopes at the bottoms of rivers, with weak hydrodynamics, heavy pollution and poor self-purification capacities for restoration biological habitats. Hydrodynamic water quality improvements are effective means ecological urban rivers. The potential fish habitat resilience a typical river network (more than 130 sections) Dianbei part China was studied. tolerant fish, Carassius auratus (C. auratus), sensitive fishes Trachidermus fasciatus (T. fasciatus) Anguilla...

Chrysomya megacephala (Fabricius) is a prevalent and synanthropic blowfly which has two sides, for being pathogenic vector, an efficient pollinator, promising resource of proteins, lipids, chitosan, biofuel et al., important forensic indicator. Moreover olfactory proteins are crucial component to function in related processes. However, the genomic platform C. remains relatively unavailable. Developmental transcriptomes eggs, larvae from 1st instar before pupa stage adults emergence egg...

Abstract Sensory system plays important roles in a wide array of insect’s behavior and physiological events, including the host landing locating, feeding, flying, sex responding, mating oviposition which happen independently sequence. The armyworm Mythimna separata (Lepidoptera: Noctuidae) migratory insect is destructive for alimentarn crop economic throughout world. Here we present high throughput sequencing head transcriptome identify members major sensory genes are crucial armyworm’s...

The intestinal bacteria of longhorn beetles would be ideal targets for pest control and lignocellulosic resources by destroying or exploiting their cellulose-degrading function. This article aims to investigate the diversity community structure oligophagous beetle Glenea cantor . Additionally, it seeks identify presence lignocellulose-degrading in gut, explore role consuming host kapok trees Bombax malabaricum In this study, bacterial from G. was examined Illumina sequencing 16S ribosomal...

A hydroponic experiment was carried out to study the modulation of exogenous reduced glutathione and selenium in antioxidant defense system microelement uptake against cadmium-toxicity maize seedlings.The results showed that 50 µM cadmium increased accumulation H2O2 malondialdehyde but plant height, root length, chlorophyll content (SPAD value) biomass.Antioxidant enzyme activities root/leaf peroxidase leaf superoxide dismutase significantly under stress, while catalase decreased...

Although parasitoids can precisely locate hidden gall-inducing insects, the host location mechanism is unknown. In this study, our aim was to clarify olfactory responses of parasitoid Quadrastichus mendeli eucalyptus volatiles induced by gall wasp Leptocybe invasa.Q. preferred from gall-damaged plants compared with those produced mechanically damaged and undamaged plants. Coupled gas chromatographic-electroantennographic detection results demonstrated that 3-carene, decanal, d-limonene,...

Incidences of thyroid disease, which has long been hypothesized to be partially caused by exposure hormone disrupting chemicals (TDCs), have rapidly increased in recent years. However, known TDCs can only explain a small portion (∼1%) vitro human transthyretin (hTTR) binding activities environmental samples, indicating the existence unknown hTTR ligands. In this study, we aimed identify major ligands employing protein Affinity Purification with Nontargeted Analysis (APNA). were detected all...

Gut bacteria are closely associated with host. Chrysomya megacephala, as a vector and resource insect, can transmit various pathogenic consume manure to produce biofertilizer larva biomass. However, the gut composition abundance of C. megacephala remain unclear.Illumina MiSeq platform was used compare bacterial community in eggs, 1-day-old larvae, 5-day-old pupae, adult females males by sequencing variation V4 region 16S ribosomal DNA gene. In total, 928 operational taxonomic units (OTUs)...

Stable reference genes are essential for accurate normalization in gene expression studies with reverse transcription quantitative polymerase chain reaction (qPCR). A synanthropic fly, Chrysomya megacephala, is a well known medical vector and forensic indicator. Unfortunately, previous did not look at the stability of used C. megacephala. In this study, level Actin, ribosomal protein L8 (Rpl8), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1α (EF1), α-tubulin (α-TUB),...

The external morphology and distribution of antennal sensilla Glenea cantor Fabricius were studied with scanning electron microscopy. antennae G. observed to be filiform, consisting scape, pedicel, flagellum (nine flagellomeres). Four distinct types sensory receptors observed, including chaetica, trichodea, basiconica, Böhm bristles. Three morphological subtypes chaetica found on the antennae, trichodea also categorized into three subtypes. Sensilla basiconica was grouped two that...

Glenea cantor Fabricius (Cerambycidae: Lamiinae) is a pest that devastates urban landscapes and causes ecological loss in southern China Southeast Asian countries where its main host kapok trees are planted. The olfactory system plays vital role mating, foraging, spawning G. as an ideal target for control. However, the mechanism of poorly understood at molecular level. In this study, we first established antennal transcriptome identified 76 olfactory-related genes, including 29 odorant...