A5045861044- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- RNA modifications and cancer
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Pancreatic function and diabetes
- Pancreatic and Hepatic Oncology Research
- Renal and related cancers
- Bacterial Genetics and Biotechnology
- FOXO transcription factor regulation
- CAR-T cell therapy research
- Cancer-related gene regulation
- Fibroblast Growth Factor Research
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
Kurchatov Institute
2012-2024
Institute of Molecular Genetics
2012-2022
Institute of Bioorganic Chemistry
2010-2019
Russian Academy of Sciences
2007-2018
Institute of Bioorganic Chemistry
2003-2014
Takara (United States)
1998
Chumakov Institute of Poliomyelitis and Viral Encephalitides
1989
Institute of Experimental Medicine
1977
Institute of Macromolecular Compounds
1967-1971
Genes that are characteristic of only certain strains a bacterial species can be great biologic interest. Here we describe PCR-based subtractive hybridization method for efficiently detecting such DNAs and apply it to the gastric pathogen Helicobacter pylori . Eighteen specific monkey-colonizing strain (J166) were obtained by against an unrelated whose genome has been fully sequenced (26695). Seven J166-specific clones had no DNA sequence match 26695 genome, 11 other mixed, with adjacent...
The nucleotide sequence of the rpoB gene Salmonella typhimurium has been determined in this work. It was compared with known sequences from other sources and conservative regions were detected. This allowed some interesting conclusions to be made about distribution functional domains bacterial RNA polymerase three‐dimensional structure its β subunit.
The primary structure of the E. coli rpoC gene (5321 base pairs) coding beta'-subunit RNA polymerase as well its adjacent segment have been determined. analysis peptides obtained by cleavage protein with cyanogen bromide and trypsin has confirmed amino acid sequence deduced from nucleotide analysis. contains 1407 residues. Its translation is initiated codon GUG terminated TAA. It detected that following terminating strikingly homologous to known sequences rho-independent terminators.
Conventional views of how proteins and other cellular components may traffic between eukaryotic cells have been challenged in recent years 1-7. Beyond the classical secretory/exocytic pathway, roles secreted or shedding microvesicles (MV) intercellular signaling attracted great attention. MVs are released by all investigated to date present practically body fluids. The diverse extracellular vesicles collectively called membrane 8. Vesicles 100 nm–1 mm diameter often referred as...
We have evaluated the key properties of polyethylenimine (PEI)-polyethylene glycol (PEG)-TAT peptide polyplex nanoparticles including their behavior in cells and compared them with transfection efficacy (TE) using 11 different cell lines. found statistically significant positive correlation between TE share 50–75 nm fraction whole mixture estimated atomic force microscopy. Variations PEG/PEI N/P ratios (PEI nitrogen to DNA phosphate ratio) enabled us find optimal combinations, which resulted...
Reporter gene analysis of HERV‐K solitary long terminal repeats (LTRs) showed that they retain detectable activity in human teratocarcinoma cells, and can direct the transcription both orientations relative to reporter gene. Deletion demonstrated possible existence alternative promoters within LTR as well a silencer‐like element U5 region. Our results indicate also all‐ trans ‐retinoic acid is capable modulating expression directed by NT2/D1 cells.
We report the first genome-wide comparison of in vivo promoter activities a group human-specific endogenous retroviruses healthy and cancerous germ line tissues. To this end, we employed recently developed technique termed genomic repeat expression monitoring. found that at least 50% long terminal repeats (LTRs) possessed activity, many them were up- or downregulated seminoma. Individual LTRs expressed markedly different levels, ranging from approximately 0.001 to 3% housekeeping beta-actin...
Gene-directed enzyme prodrug therapy (GDEPT) represents a technology to improve drug selectivity for cancer cells. It consists of delivery into tumor cells suicide gene responsible in situ conversion cytotoxic metabolites. Major limitations GDEPT that hinder its clinical application include inefficient and poor activation by enzymes. We tried overcome these constraints through combination with immunomodulating therapy. Viral vectors dominate present-day trials due efficient transfection...
Humans share about 99% of their genomic DNA with chimpanzees and bonobos; thus, the differences between these species are unlikely to be in gene content but could caused by inherited changes regulatory systems. Endogenous retroviruses (ERVs) comprise approximately 5% human genome. The LTRs ERVs contain many sequences, such as promoters, enhancers, polyadenylation signals factor-binding sites. Thus, they can influence expression nearby genes. All known human-specific belong HERV-K (human ERV)...
We developed a technique called GREM (Genomic Repeat Expression Monitor) that can be applied to genome-wide isolation and quantitative analysis of any kind transcriptionally active repetitive elements. Briefly, the includes three major stages: (i) generation transcriptome wide library cDNA 5′ terminal fragments, (ii) selective amplification repeat-flanking genomic loci (iii) hybridization amplicon with subsequent cloning cDNA-genome hybrids. The sequences obtained serve as 'tags' for...
Here we directly compared gene expression profiles in human esophageal squamous cell carcinomas and fetal esophagus development. We used the suppression subtractive hybridization technique to subtract cDNAs prepared from tumor normal samples. cDNA sequencing reverse transcription polymerase chain reaction (RT-PCR) analysis of RNAs revealed 10 differentially transcribed genes: CSTA, CRNN, CEACAM1, MAL, EMP1, ECRG2, SPRR downregulated, PLAUR, SFRP4, secreted protein that is acidic rich...