A5068797455- Vitamin D Research Studies
- Bone Metabolism and Diseases
- Metabolism, Diabetes, and Cancer
- Estrogen and related hormone effects
- Vitamin C and Antioxidants Research
- Ion Channels and Receptors
- Pancreatic function and diabetes
- Birth, Development, and Health
- Cancer, Stress, Anesthesia, and Immune Response
- Digestive system and related health
- Growth Hormone and Insulin-like Growth Factors
- Drug Transport and Resistance Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Microbial Metabolism and Applications
- Metabolism and Genetic Disorders
- Magnesium in Health and Disease
- Adenosine and Purinergic Signaling
- Vitamin K Research Studies
- Ion channel regulation and function
- Thyroid Disorders and Treatments
- Free Radicals and Antioxidants
- Genomics, phytochemicals, and oxidative stress
- Protein Kinase Regulation and GTPase Signaling
- Sexual Differentiation and Disorders
- Bone health and treatments
Universidad Nacional de Córdoba
2007-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2023
Instituto de Investigaciones en Ciencias de la Salud
2014-2023
National Research Council of Science and Technology
2014
GTx (United States)
2014
University at Albany, State University of New York
2011-2013
Instituto de Biotecnología y Biología Molecular
2013
Research Centre in Biological Chemistry of Córdoba
2012
University of Notre Dame
2007
Universidad Nacional del Sur
1996-2006
Inhibition of dendritic cell maturation and activation, together with abnormal functioning cell-mediated immunity, has been reported in chronic spinal cord injury (SCI). The development immune-based therapies could: 1) prevent or slow down limit further tissue damage SCI, 2) promote regeneration. To identify novel candidate molecular pathways mediating SCI-induced immune changes, we performed whole-genome microarray pathway analyses. Subjects motor complete SCI (> 2 years post-injury)...
Direct effects of parathyroid hormone (PTH) on calcium uptake by isolated rat duodenal cell preparations enriched in enterocytes were investigated. PTH significantly stimulated enterocyte 45 Ca 2+ influx a time-dependent (1–10 min) manner and at all doses tested (2 × 10 −13 to −7 M). The channel antagonists verapamil (10 μM) nitrendipine (1 completely blocked the stimulation −8 markedly increased cAMP levels cells (88, 167, 67%, after 1, 2, 3 min, respectively). In agreement with these...
Children born small for gestational age (SGA) are prone to developing obesity, insulin resistance and type 2 diabetes. Adiponectin leptin adipocytokines associated with sensitivity parameters. We aimed relate serum adiponectin levels parameters in prepuberal SGA children without catch-up growth (SGA+CUG; SGA-CUG, respectively) analyze the usefulness of these as early markers resistance. analysed adiponectin, proinsulin, leptin, factors, insulin, HOMA IR βcell 23 SGA+CUG, 26 SGA-CUG compared...
The involvement of the phospholipase C (PLC) pathway in non-genomic regulation duodenal cell Ca2+ concentration by 17β-oestradiol was investigated. PLC inhibitors neomycin (0.5 mM) and U-73122 (2 µM) suppressed stimulatory effect 0.1 nM on 45Ca2+ influx into enterocytes isolated from rat duodenum. hormone (1 pM to 10 nM) increased formation 1,2-diacylglycerol a biphasic pattern, characterized an early peak at 45 s (+82%) later 5 min (+46%). Both first but were unable block min....
The prognosis and incidence of colon cancer are linked to vitamin D3 serum levels. To evaluate the effects D,L-buthionine-S,R-sulfoximine (BSO), 1,25(OH)2D3 their combination on intestinal Caco-2 cell growth, elucidate possible cellular mechanisms involved in antiproliferative action, determine whether BSO acts as a sensitizer treatment, enabling minimization toxic caused by high doses steroid. Human cells were treated with 1,25(OH)2D3, BSO, both, or vehicle. Cell proliferation was evaluated...
Bone resorption and organelle homeostasis in osteoclasts require specialized intracellular trafficking. Sorting nexin 10 (Snx10) is a member of the sorting family proteins that plays crucial roles cargo endosomal pathway by its binding to phosphoinositide(3)phosphate (PI3P) localized early endosomes. We others have shown previously gene encoding Snx10 required for osteoclast morphogenesis function, as from humans mice lacking functional are dysfunctional. To better understand role mechanisms...
Abstract 1α,25‐Dihydroxyvitamin D 3 [1α,25(OH) 2 ], the hormonally active form of vitamin , has been shown to be a potent negative growth regulator breast cancer cells both in vitro and vivo. 1α,25(OH) acts through two different mechanisms. In addition regulating gene transcription via its specific intracellular receptor (vitamin receptor, VDR), induces rapid, non‐transcriptional responses involving activation transmembrane signal transduction pathways, like factors peptide hormones. The...
We have investigated the effects of aging on parathyroid hormone (PTH) modulation intracellular calcium homeostasis and their relationship to signal transduction pathways in isolated rat duodenal cells (enterocytes). PTH (10−8–10−9 M) increased enterocyte 45Ca2+ influx Ca2+ concentration ([Ca2+]i) a greater extent (twofold 50%, respectively) aged (24 months) than young (3 animals. The [Ca2+]i response old was slower, lacking early phase changes cytosolic Ca2+. induced by prevented protein...
Abstract Little is known about the regulation of cytosolic calcium Ca 2+ levels ([Ca ] i ) in breast cancer cells. We investigated existence capacitative entry (CCE) tumorigenic cell line MCF‐7 and its responsiveness to ATP. cells express purinergic receptors as well estrogen (ER). Depletion stores with thapsigargin (TG, 500 nM) or ATP (10 μM) absence extracellular , resulted a rapid transient elevation [Ca . After recovery basal levels, readmission (1.5 mM) medium increased influx (twofold...
Calcitriol or 1,25(OH)2D3 is a negative growth regulator of breast cancer cells. The aim this study was to determine whether L-buthionine-S,R-sulfoximine, glutathione-depleting drug, modifies the antiproliferative effects on MCF-7 For comparison, we included studies in cells selected for vitamin D resistance and human mammary epithelial transformed with SV40 ras. Our data indicate that L-buthionine-S,R-sulfoximine enhances inhibition all cell lines. This effect mediated by ROS leading...
The aim of this study was to determine new insights into the molecular mechanisms involved in antiproliferative action menadione + calcitriol (MEN+D) on MCF-7 cells. After 24 h, MEN+D inhibited cell growth but not observed with each single treatment. combined drugs reduced mitochondrial respiration at that time, as judged by an increase proton leak and a decrease ATP generation coupling efficiency. At longer times, 48 or 96 either D MEN proliferation, effect higher when both were used...
We have previously shown that chitosan stimulates anthraquinone synthesis in Rubia tinctorum L. cells through activation of the PLC\PKC, PI3K, MAPK and Ca 2+ messenger systems. In view this evidence, we now investigated whether guanine nucleotide‐binding G‐proteins are part signal transduction mechanism which mediates elicitor action. The G‐protein agonists mastoparan, AlF 4 – GTPyS increased levels to same extent as chitosan. No additive effects were observed when cultured R. treated with...
Clinical, biochemical and genetic analysis related to bone mineral density (BMD) were carried out in children born small for gestational age (SGA) that failed achieve postnatal catch-up growth (CUG), SGA completed CUG adequate (AGA) children. Serum IGF-I, IGF-II, IGF binding protein-3 acid-labile subunit lower the SGA–CUG as compared with other groups. Frequencies of polymorphic variants vitamin D receptor, estrogen receptor collagen genes similar among The genotype 194–192 IGF-I gene was...