A5103086868- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- 3D Printing in Biomedical Research
- Molecular Biology Techniques and Applications
- Nanofabrication and Lithography Techniques
- Algal biology and biofuel production
- Micro and Nano Robotics
- Electrowetting and Microfluidic Technologies
- Organometallic Complex Synthesis and Catalysis
- Advanced Biosensing Techniques and Applications
- Electrochemical sensors and biosensors
- Click Chemistry and Applications
- Nanoparticles: synthesis and applications
- Cellular Mechanics and Interactions
- Asymmetric Hydrogenation and Catalysis
- Synthetic Organic Chemistry Methods
- Advanced Materials and Mechanics
- Graphene and Nanomaterials Applications
- Chemical Synthesis and Analysis
- Electrochemical Analysis and Applications
- Carbon dioxide utilization in catalysis
- Molecular Communication and Nanonetworks
Kyung Hee University
2016-2021
Korea Advanced Institute of Science and Technology
2002-2016
Intelligent Synthetic Biology Center
2010-2012
University of California, Berkeley
2007-2009
Columbia University
2002-2004
Don′t FRET when I GO: Sensitive and selective rotavirus detection is achieved by using the photoluminescence of a graphene oxide (GO) array. The target cell was captured rotavirus-specific antibody immobilized on GO array, binding event monitored observing fluorescence quenching that results from resonance energy transfer (FRET) between gold nanoparticles linked to antibodies (see picture).
Homogeneous blue luminescent MoS2 quantum dots are fabricated by using a lithium intercalation method from nanoparticles, and the unique photoluminescence property is utilized in Alexa Fluor 430-dsDNA-MoS2 FRET system, demonstrating dual function of as donor an acceptor.
"Click chemistry" 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The successfully used as a primer sequencing products with single-base resolution capillary electrophoresis sequencer laser-induced fluorescence detection.
A portable forensic genetic analysis system consisting of a microfluidic device for amplification and separation short tandem repeat (STR) fragments as well an instrument chip operation four-color fluorescence detection has been developed. The microdevice performs polymerase chain reaction (PCR) in 160-nL chamber capillary electrophoresis (CE) 7-cm-long channel. instrumental design integrates PCR thermal cycling, electrophoretic separation, pneumatic valve fluidic control, laser excited...
A metal-nanoparticle factory: Various metal nanoparticles with tailored optical, electronic, chemical, and magnetic properties were synthesized in vivo a size-tunable manner recombinant E. coli (see picture) through interaction the metal-binding protein metallothionein peptide phytochelatin, which was by phytochelatin synthase. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made...
A laboratory-on-a-chip system for pathogen detection is presented that integrates cell preconcentration, purification, polymerase chain reaction (PCR), and capillary electrophoretic (CE) analysis. The microdevice composed of micropumps valves, a capture structure, 100 nL PCR reactor, 5 cm long CE column amplicon separation. Sample volumes ranging from 10 to μL are introduced driven through fluidized bed magnetically constrained immunomagnetic beads where the target cells captured. After...
Rotaviren auf der Spur: Die empfindliche und selektive Detektion von gelingt mithilfe Photolumineszenz eines Graphenoxid(GO)-Arrays. Zielzelle wurde vom Rotavirus-spezifischen, dem GO-Array immobilisierten Antikörper eingefangen, Bindevorgang anhand Fluoreszenzlöschung verfolgt, die aus resonanten Fluoreszenzenergietransfer (FRET) zwischen GO Goldnanopartikeln an den Antikörpern resultiert (siehe Bild).
Here we report an integrated microdevice consisting of efficient passive mixer, a magnetic separation chamber, and capillary electrophoretic microchannel in which DNA barcode assay, target pathogen separation, analysis were performed sequentially within 30 min for multiplex detection at the single-cell level. The intestine-shaped serpentine 3D micromixer provides high mixing rate to generate particle–pathogenic bacteria–DNA labelled AuNP complexes quantitatively. After purification those...
Einheitliche Leistung: Ein mikrofluidischer Tröpfchengenerator verkapselt eine stets gleiche Zahl genmanipulierter E.-coli-Zellen mit denselben Konzentrationen an Fe- und Mn-Ionen in Tröpfchen identischer Größe. In den Zellen werden daraufhin biogene paramagnetische FeMn-Nanopartikel synthetisiert.