A5007916501- Quantum Chromodynamics and Particle Interactions
- Rare-earth and actinide compounds
- Glycosylation and Glycoproteins Research
- Particle physics theoretical and experimental studies
- Monoclonal and Polyclonal Antibodies Research
- X-ray Diffraction in Crystallography
- High-Energy Particle Collisions Research
- Nuclear Physics and Applications
- Inorganic and Organometallic Chemistry
- Crystallization and Solubility Studies
- Organic Chemistry Cycloaddition Reactions
- Magnetic Properties of Alloys
- Inorganic Chemistry and Materials
- Iron-based superconductors research
- Synthesis and Reactions of Organic Compounds
- Synthesis and Biological Evaluation
- Advanced Chemical Physics Studies
- Chemical Synthesis and Analysis
- Galectins and Cancer Biology
- Crystallography and molecular interactions
- Nonlinear Optical Materials Research
- Crystal structures of chemical compounds
- Asymmetric Synthesis and Catalysis
- Protein purification and stability
- Metal complexes synthesis and properties
Institute of Nuclear Physics, Polish Academy of Sciences
2010-2022
Roche Pharma AG (Germany)
2002-2021
Rzeszów University
2013-2020
Institute of Physics
2013-2020
Institute of Nuclear Physics
2016
Roche (Switzerland)
2011-2015
UCLouvain
2014
CS Diagnostics
2002-2013
Institute of Molecular Physics of the Polish Academy of Sciences
2005
Max Planck Institute of Biochemistry
2005
We describe a generic approach to assemble correctly two heavy and light chains, derived from existing antibodies, form human bivalent bispecific IgG antibodies without use of artificial linkers. Based on the knobs-into-holes technology that enables heterodimerization correct association chains their cognate is achieved by exchange heavy-chain light-chain domains within antigen binding fragment (Fab) one half antibody. This "crossover" retains antigen-binding affinity but makes arms so...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelective non-nucleoside HIV-1 reverse transcriptase inhibitors. New 2,3-dihydrothiazolo[2,3-a]isoindol-5(9bH)-ones and related compounds with anti-HIV-1 activityAlfred Mertens, Harald Zilch, Bernhard Koenig, Wolfgang Schaefer, Thomas Poll, Kampe, Hans Seidel, Ulrike Leser, Herbert LeinertCite this: J. Med. Chem. 1993, 36, 17, 2526–2535Publication Date (Print):August 1, 1993Publication History Published online1 May 2002Published inissue 1 August...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInteractions between nonconjugated .pi.-systemsRolf Gleiter and Wolfgang SchaeferCite this: Acc. Chem. Res. 1990, 23, 11, 369–375Publication Date (Print):November 1, 1990Publication History Published online1 May 2002Published inissue 1 November 1990https://pubs.acs.org/doi/10.1021/ar00179a004https://doi.org/10.1021/ar00179a004research-articleACS PublicationsRequest reuse permissionsArticle Views394Altmetric-Citations160LEARN ABOUT THESE...
We revisit the Wigner function approach to impact parameter dependent dilepton pair production developed in [M. Klusek-Gawenda, WS, A. Szczurek Phys. Lett. B 814 (2021) 136114]. study distribution of angle between difference and sum lepton transverse momenta, show how it relates orbital angular momentum leptons. The dependence on paramter is discussed, we also present different components t-channel. A brief comparison similar distributions diffractive quark will be presented.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEvidence for a strong .sigma.,.pi. interaction in 3,4,7,8-tetrasilacycloocta-1,5-diyne and 3,4,7,8,11,12-hexasilacyclododeca-1,5,9-triyneRolf Gleiter, Wolfgang Schaefer, Hideki SakuraiCite this: J. Am. Chem. Soc. 1985, 107, 11, 3046–3050Publication Date (Print):May 1, 1985Publication History Published online1 May 2002Published inissue 1 1985https://pubs.acs.org/doi/10.1021/ja00297a009https://doi.org/10.1021/ja00297a009research-articleACS...
The quadroma antibody represents the first attempt to produce a bispecific heterodimeric IgG by somatic fusion of 2 hybridoma cells each expressing monoclonal antibodies with distinctive specificities. However, because random heavy and light chain pairing, desired functional only small fraction protein produced. Subsequently, knobs-into-holes (KiH) approach was developed enforce correct heterodimerization. Assuming equimolar expression 4 unmodified chains comprising chains, statistical...
Bispecific antibodies are considered as a promising class of future biotherapeutic molecules. They comprise binding specificities for two different antigens, which may provide additive or synergistic modes action. There is wide variety design alternatives such bispecific antibodies, including the "CrossMab" format. CrossMabs contain domain crossover in one antigen-binding (Fab) parts, together with "knobs-and-holes" approach, to enforce correct assembly four polypeptide chains into an...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTetracyclo[8.2.2.22,5.26,9]octadeca-1,5,9-trieneJohn E. McMurry, Gregory J. Haley, James R. Matz, Jon C. Clardy, Van Duyne, Rolf Gleiter, Wolfgang Schaefer, and David H. WhiteCite this: Am. Chem. Soc. 1984, 106, 17, 5018–5019Publication Date (Print):August 1, 1984Publication History Published online1 May 2002Published inissue 1 August 1984https://pubs.acs.org/doi/10.1021/ja00329a071https://doi.org/10.1021/ja00329a071research-articleACS...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and chemistry of tetracyclo[8.2.2.22,5.26,9]-1,5,9-octadecatrieneJohn E. McMurry, Gregory J. Haley, James R. Matz, Jon C. Clardy, Gregory. Van Duyne, Rolf. Gleiter, Wolfgang. Schaefer, David H. WhiteCite this: Am. Chem. Soc. 1986, 108, 11, 2932–2938Publication Date (Print):May 1, 1986Publication History Published online1 May 2002Published inissue 1...
Technologies for the production of bispecific antibodies need to overcome two major challenges. The first one is correct heavy chain assembly, which was solved by knobs-into-holes technology or charge interactions in CH3 domains. second challenge light assembly. This can be engineering Fab-arm interfaces applying immunoglobulin domain crossover approach. There are three different crossovers possible, namely Fab-arm, constant and variable crossovers. CrossMabCH1–CL exchange does not lead...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHydrocarboxymethylation-an Attractive Route from Olefins to Fatty Acid Esters?Kurt Kosswig and Wolfgang SchaeferCite this: Ind. Eng. Chem. Prod. Res. Dev. 1980, 19, 3, 330–334Publication Date (Print):September 1, 1980Publication History Published online1 May 2002Published inissue 1 September 1980https://pubs.acs.org/doi/10.1021/i360075a011https://doi.org/10.1021/i360075a011research-articleACS PublicationsRequest reuse permissionsArticle...
Tb dihydride TbH2+x has been investigated for −0.05<x<0.12 by means of magnetic susceptibility and neutron diffraction measurements. It is shown that both the structure Néel temperature TN depend strongly on x. For x≊−0.05, a commensurate antiferromagnetic appears below 15.8 K. higher concentrations different incommensurate structures are obtained which stable from down to 4 The transition into an ascribed random occupation some octahedral sites in fcc lattice x≳−0.05.
An increasing number of bispecific therapeutic antibodies are progressing through clinical development. The Knob-into-Hole (KiH) technology uses complementary mutations in the CH3 region antibody Fc fragment to achieve heavy chain heterodimerization. Here we describe X-ray crystal structures glycosylated and disulfide-engineered heterodimeric KiH its homodimeric Knob-Knob Hole-Hole side products. heterodimer structure confirms design principle supports hypothesis that glycosylation...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMetabolism of pentachlorophenol in cell suspension cultures soybean (Glycine max L.) and wheat (Triticum aestivum L.). General results isolation lignin metabolitesDierk Scheel, Wolfgang Schaefer, Heinrich Sandermann Jr.Cite this: J. Agric. Food Chem. 1984, 32, 6, 1237–1241Publication Date (Print):November 1, 1984Publication History Published online1 May 2002Published inissue 1 November...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSigma/pi interactions in [2.2]paracyclophanes. PE investigations on octamethyltetrasila[2.2]paracyclophane and octamethyltetrasila[2.2](2,5)thiophenophane -furanophaneRolf. Gleiter, Wolfgang. Schaefer, Gerhard. Krennrich, Hideki. SakuraiCite this: J. Am. Chem. Soc. 1988, 110, 13, 4117–4120Publication Date (Print):June 1, 1988Publication History Published online1 May 2002Published inissue 1 June...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectronic control of stereoselectivity. 27. The effect apical spirocyclopropane substitution on the stereochemical course Diels-Alder cycloadditions to norbornyl-fused diene systemsLeo A. Paquette, Kenneth E. Green, Rolf Gleiter, Wolfgang Schaefer, and Judith C. GallucciCite this: J. Am. Chem. Soc. 1984, 106, 26, 8232–8240Publication Date (Print):December 1, 1984Publication History Published online1 May 2002Published inissue 1 December...