A5101790290- Malaria Research and Control
- Mosquito-borne diseases and control
- Orthopedic Surgery and Rehabilitation
- Cardiac Arrest and Resuscitation
- HIV Research and Treatment
- Shoulder and Clavicle Injuries
- Venomous Animal Envenomation and Studies
- Kidney Stones and Urolithiasis Treatments
- Bone fractures and treatments
- Esophageal and GI Pathology
- Trauma and Emergency Care Studies
- Neuroblastoma Research and Treatments
- Nerve Injury and Rehabilitation
- Psychosomatic Disorders and Their Treatments
- Entomological Studies and Ecology
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Abdominal Trauma and Injuries
- Urological Disorders and Treatments
- Aquaculture disease management and microbiota
- Signaling Pathways in Disease
- Sleep and Work-Related Fatigue
- Multiple Myeloma Research and Treatments
- Pediatric Urology and Nephrology Studies
- Neuroendocrine Tumor Research Advances
- Streptococcal Infections and Treatments
Indraprastha Apollo Hospitals
2024
PBL Assay Science (United States)
2023
Army Hospital Research and Referral
2021-2022
International Centre for Genetic Engineering and Biotechnology
2012-2021
All India Institute of Medical Sciences
2020
All India Institute of Medical Sciences Bhopal
2020
All India Institute of Medical Sciences Raipur
2020
Universal College of Medical Sciences
2014-2019
Central Council for Research in Yoga & Naturopathy
2019
Central University of Gujarat
2019
Significance Plasmodium falciparum reticulocyte binding-like homologous protein 5 (PfRH5) is a leading blood-stage malaria vaccine candidate that elicits potent strain-transcending invasion inhibitory antibodies. However, it lacks both transmembrane domains and GPI-anchor thus anchored to the merozoite surface through an unknown mechanism. We have demonstrated PfRH5 its known partner, PfRH5-interacting (PfRipr), associates with conserved GPI-anchored protein, Cysteine-rich protective antigen...
ABSTRACT Plasmodium falciparum reticulocyte binding-like homologous protein 5 (PfRH5) is an essential merozoite ligand that binds with its erythrocyte receptor, basigin. PfRH5 attractive malaria vaccine candidate, as it expressed by a wide number of P. strains, cannot be genetically disrupted, and exhibits limited sequence polymorphisms. Viral vector-induced antibodies potently inhibited invasion. However, has been challenge to generate full-length recombinant in bacterial-cell-based...
Blood-stage malaria vaccines that target single Plasmodium falciparum antigens involved in erythrocyte invasion have not induced optimal protection field trials. vaccine development has faced two major hurdles, antigenic polymorphisms and molecular redundancy, which led to an inability demonstrate potent, strain-transcending, invasion-inhibitory antibodies. Vaccines multiple invasion-related parasite proteins may inhibit more efficiently. Our approach is develop a receptor-blocking...
Epiphrenic diverticulum is a type of pulsion and are often asymptomatic small in size. Symptomatic large epiphrenic dicverticulum has very low prevalence. We present rare case giant oesophageal around 9 cm Open thoracotomy or thorascopic procedure generally require for the treatment. But with help robotic surgery, even can be excised transhiataly good early postoperative recovery. The system an advantage better visualization degree movement which ease dissection diverticulum. Large upper...
ABSTRACT A Plasmodium falciparum chimeric protein, PfMSP-Fu 24 , was constructed by genetically coupling immunodominant, conserved regions of two merozoite surface proteins, the 19-kDa region C-terminal protein 1 (PfMSP-1 19 ) and an 11-kDa 3 (PfMSP-3 11 ), to augment immunogenicity potential these blood-stage malaria vaccine candidates. Here we describe improved, efficient, scalable process produce high-quality . The produced in Escherichia coli SHuffle T7 Express lysY cells that express...
ABSTRACT Fomites are known to play a role in the transmission of pathogens. Quantitative analysis parameters that affect sample recovery efficiency (SRE) at limit detection viruses on fomites will aid improving quantitative microbial risk assessment (QMRA) and infection control. The variability SRE as function fomite type, surface area, sampling time, application media, relative humidity (rH), wetting agent was evaluated. To quantify SRE, bacteriophage P22 applied onto average densities 0.4...
A main criterion to identify malaria vaccine candidates is the proof that acquired immunity against them associated with protection from disease. The age of studied individuals, heterogeneous exposure, and assumption maintenance a baseline immune response can confound these associations. Immunoglobulin G/immunoglobulin M (IgG/ IgM) levels were measured by Luminex® in Mozambican children monitored for clinical birth until 3 years age, together functional antibodies. Studied pre-erythrocytic...
Plasmodium falciparum cysteine-rich protective antigen (CyRPA) is a conserved component of an essential erythrocyte invasion complex (RH5/Ripr/CyRPA) and target potent cross-strain parasite-neutralizing antibodies. While naturally acquired human RH5 antibodies have been functionally characterized, there are no similar reports on CyRPA. Thus, we analyzed the activity CyRPA In this regard, were measured purified from malaria-infected plasma obtained patients in central India for their parasite...
Background Femoral-shaft fractures are among the most common of lower extremity in children. There several different options for treating femoral-shaft Elastic stable intramedullary nailing (ESIN) has become standard treatment shaft femur children reasons including mini-invasive surgery, no need casting, early mobilization and discharge as well growing concerns toward cost-effectiveness.Objective To demonstrate effectiveness fixation fracture skeletally immature using titanium elastic...
Abstract Background Targeting multiple key antigens that mediate distinct Plasmodium falciparum erythrocyte invasion pathways is an attractive approach for the development of blood-stage malaria vaccines. However, challenge to identify antigen cocktails elicit potent strain-transcending parasite-neutralizing antibodies efficacious at low immunoglobulin G concentrations feasible achieve through vaccination. Previous reports have screened inhibitory primarily against well adapted laboratory...
Development of fusion chimeras as potential vaccine candidates is considered an attractive strategy to generate effective immune responses more than one antigen using a single construct. Here, we described the design, production, purification and antigenicity chimera (PfAMSP-Fu35), comprised immunologically relevant regions three target malaria antigens, PfAARP, PfMSP-3 PfMSP-1. The recombinant PfAMSP-Fu35 expressed soluble protein purified homogeneity with ease at yield ~ 7 mg L-1....
INTRODUCTION: Hand injuries are common and responsible for a significant hospital emergency visit most of which seen by non hand specialists. The pattern the varies from place to place. It is pertinent that those who may come across patients understand this pattern. MATERIALS & METHODS: A retrospective review records presenting with unit in OPD over two year period was carried out highlighting injury.RESULTS: cause injury these fodder cutter machines 53 (30.6%) followed industrial 36...
Neuroendocrine tumors (NETs) of the extrahepatic bile ducts are extremely rare. They heterogeneous entities varied histopathological features. The pathological types vary greatly with regards to biological behavior and prognosis. We presented such a case silent tumor biliary tract neuroendocrine origin in middle-aged female nonspecific abdominal pain without any clinically obvious signs which on radiological imaging revealed hepatic confluence metastatic deposits liver for she underwent left...
Pancreatic cancers usually metastasize through the lymphoid system to organs such as lung, liver, bone and spleen. Ovarian metastasis in pancreatic is extremely rare, hence, it difficult distinguish between primary metastatic ovarian tumors, especially tumors with a source from GIT & Hepatobiliary system. We present case of periampullary carcinoma middle-aged female who presented complaints abdominal pain, constipation, yellowish discoloration eyes dark colored urine along loss appetite...
When a more proximal section of the bowel (intussusceptum) invaginates into distal (intussuscipiens), it results in intussusception. Changes intestinal peristalsis at intraluminal lesion, which serves as lead point for intussusceptum, is assumed to be mechanism. Adult cases intussusception are uncommon; they make up about 1% all obstructions. We describe case colo-colic caused by an adenocarcinoma sigmoid colon. The most frequent urgent condition children intussusception, although extremely...
BACKGROUND:There is much debate regarding the optimal treatment of displaced distal radius fracture. The purpose this prospective study was to compare functional and radiological outcomes radial fracture treated with cast or percutaneous pin.MATERIALS & METHODS: Sixty patients all age groups non comminuted extra-articular partially articular were included in study. After randomization, thirty pins. For pin group below elbow volar slab applied for three weeks. weeks, removed wrist...
BACKGROUND: Treatment of Frozen shoulder (Adhesive Capsulitis) is mainly nonoperative. Intra-articular steroid injections and physiotherapy are one the most effective useful treatment. Even though intra-articular very in inflammatory phase disease; patients reluctant to move their affected for fear pain. Thus, they do not follow exercise program properly resulting poor outcomes.OBJECTIVE: If pain could be reduced, outcome treatment can improve. Lignocaine, when combined with injection, plays...
Introduction: Fodder cutter machine injuries of the hand are common accidents faced in agriculture sectors requiring a specialist and contributing to severe disability morbidity which directly affect functional capability an individual. However studies on these have not been conducted this country (Nepal) so we study identify epidemiological risk factors (age, sex, site injury, day injury severity injury) attending Universal College Medical Sciences (UCMS-TH), Bhairahawa. Material Methods:...
INTRODUCTION: Toxic keratoconjunctivitis is a frequently encountered problem in the subspecialty of cornea. can occur as complication exposure to various substances. due many chemicals and medications have been well described literature. The present study was carried out explore clinical profile toxic caused by insect an unknown cause tertiary care center Lumbini zone western region Nepal. METHODS: It prospective observational conducted cornea clinic Eye Institute, Bhairahawa, Nepal from 1...
Actinomycotic mycetoma is a disease of the tropical region and usually presents as chronic, suppurative deforming granulomatous infection. We present an unusual case actinomycotic abdominal wall that was found to infiltrate into bowel. A 51 year-old man presented with pain swelling in left flank 2-year duration. Even after comprehensive preoperative evaluation advanced radiological imaging, biochemistry pathology, diagnosis could not be arrived at. Histopathological examination excised...
INTRODUCTION:-Backache is a common problem with about 70% of people in developed countries experience low back pain at some time their lives. A number studies done previously shows that there high prevalence depression patients chronic backache. This study was carried out to find the backache Nepalese subjects.
 MATERIAL AND METHODS:-This cross-sectional descriptive conducted Universal College Medical Sciences, Bhairahawa, Nepal. Patients presenting Orthopaedics OPD history lasting for...