A5042292670- Probiotics and Fermented Foods
- Oral microbiology and periodontitis research
- Streptococcal Infections and Treatments
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Biochemical and Structural Characterization
- Bacterial Identification and Susceptibility Testing
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Microbial Metabolites in Food Biotechnology
- Infective Endocarditis Diagnosis and Management
- HIV/AIDS oral health manifestations
- Dental Health and Care Utilization
- Oral Health Pathology and Treatment
- Bacterial Genetics and Biotechnology
- Salivary Gland Disorders and Functions
- Enzyme Production and Characterization
- Dental materials and restorations
- Pneumonia and Respiratory Infections
- Dental Erosion and Treatment
- Antimicrobial agents and applications
- Forensic and Genetic Research
- Clostridium difficile and Clostridium perfringens research
- ATP Synthase and ATPases Research
- Protein Hydrolysis and Bioactive Peptides
University of Otago
2012-2024
Smith College
2016
University of Massachusetts Amherst
2016
Charité - Universitätsmedizin Berlin
2007
German Institute of Human Nutrition
2007
Dunedin Public Hospital
2006
Recent research has provided mechanistic insight into the important contributions of gut microbiota to vertebrate biology, but questions remain about evolutionary processes that have shaped this symbiosis. In present study, we showed in experiments with gnotobiotic mice evolution Lactobacillus reuteri rodents resulted emergence host specialization. To identify genomic events marking adaptations murine host, compared genome rodent isolate L. 100-23 human F275, and identified hundreds genes...
Orthodontic braces can impede oral hygiene and promote halitosis. The aim of the study was to investigate effect probiotic Streptococcus salivarius M18 on indices halitosis in patients wearing orthodontic braces. a prospective, randomized, triple-blind, placebo-controlled trial. Patients undergoing fixed treatment were randomly allocated group (n = 32) placebo 32). consumed 2 lozenges d−1 for one month. Assessments taken at baseline, end intervention, 3 month follow-up. outcome measures...
ABSTRACT Lactobacilli are common inhabitants of the gastrointestinal tracts mammals and have received considerable attention due to their putative health-promoting properties. Little is known about traits that enhance ability these bacteria inhabit tract. In this paper we describe development application a strategy based on in vivo expression technology (IVET) enables detection Lactobacillus reuteri genes specifically induced murine gut. A plasmid-based system was constructed containing ′...
ABSTRACT Streptococcus mutans UA159, the genome sequence reference strain, exhibits nonlantibiotic mutacin activity. In this study, bioinformatic and mutational analyses were employed to demonstrate that antimicrobial repertoire of strain UA159 includes IV (specified by nlm locus) a newly identified bacteriocin, V (encoded SMU.1914c).
Streptococcus salivarius is a Gram-positive bacterial commensal and pioneer colonizer of the human oral cavity. Many strains produce ribosomally synthesized proteinaceous antibiotics (bacteriocins), some have been developed for use as probiotics. Here, we present draft genome sequence bacteriocin-producing probiotic S. strain M18.
To determine the relationship between periodontal pathogen load and anti-human heat shock protein 60 (hHSP60) antibodies in patients with established cardiovascular disease (CVD).Participants were (n = 74) a previous hospital admission for myocardial infarction. Concurrent of Porphyromonas gingivalis, Fusobacterium nucleatum, Tannerella forsythia Aggregatibacter actinomycetemcomitans was determined using quantitative real-time PCR. Serum to these pathogens, GroEL hHSP60 an ELISA.There trend...
Dental caries is an infectious disease that continuing to increase in prevalence, reducing the quality of life for millions worldwide as well causing considerable expense, with estimated US$108 billion spent on dental care USA each year. Oral probiotics are now being investigated determine whether they could play a role prevention and treatment this disease. Streptococcus salivarius strain JH potential probiotic candidate produces multiple proteinaceous antimicrobials (bacteriocins),...
ABSTRACT Streptococcus mutans UA159, the genome sequence reference strain, exhibits nonlantibiotic bacteriocin (mutacin) activity. In this study, we have combined bioinformatic and mutational analyses to identify ABC transporter designated NlmTE, which is required for mutacin biogenesis in strain UA159 as well another producer, S. N.
The production of streptocins STH(1) and STH(2) by Streptococcus gordonii DL1 (Challis) is directly controlled the competence regulon, which requires intact comR comAB loci. streptocin (sth) locus comprises two functional genes, sthA sthB. Whereas activity alone, depends on both genes.
Dysgalacticin is a novel bacteriocin produced by Streptococcus dysgalactiae subsp. equisimilis strain W2580 that has narrow spectrum of antimicrobial activity directed primarily against the principal human streptococcal pathogen pyogenes. Unlike many previously described bacteriocins Gram-positive bacteria, dysgalacticin heat-labile 21.5 kDa anionic protein kills its target without inducing lysis. The N-terminal amino acid sequence...
Streptococcus uberis, a causal agent of bovine mastitis, produces ubericin A, 5.3-kDa class IIa (pediocin-like) bacteriocin, which was purified and characterized. The uba locus comprises two overlapping genes: ubaA (ubericin A precursor peptide) ubaI (putative immunity protein). Ubericin is the first streptococcal bacteriocin to be
The aims of this study were to investigate the clinical effectiveness and patient acceptability a modified glass ionomer cement placed using atraumatic restorative treatment (ART) technique treat root caries, carry out microbiological analysis restored sites. Two clinically visible surface carious lesions per participant ART. One was with commercial (GIC) (ChemFil® Superior, DENTSPLY, Konstonz, Germany) which acted as control. other lesion same GIC 5% chlorhexidine digluconate (GIC-CHX;...
Abstract The oral microbiome is ecologically diverse, complex, dynamic, and little understood. We describe the microbiota of four habitats in a birth cohort at age 32 examine differences by sex, hygiene, current smoking status, dental caries, periodontal health. Oral biofilm samples collected from anterior labial supragingival, posterior lingual subgingival, tongue sites 841 Dunedin Multidisciplinary Health Development Study members were analysed using checkerboard DNA‐DNA hybridization;...
Members of the genus Streptococcus inhabit a variety sites in humans and other animals some species are prolific producers proteinaceous antibiotics (bacteriocins). As little is known about (i) streptococci inhabiting domestic pets, (ii) whether novel bacteriocin-producing can be isolated from aim this study to address these gaps research literature. In study, equinus MDC1, healthy dog, was found exhibit potent antibacterial activity against Micrococcus luteus simultaneous antagonism assay,...
Background and Objective There is a paucity of data in relation to the possible emergence triclosan ( TCS )‐resistant bacteria following long‐term exposure toothpaste. Therefore, this study investigated whether continuous toothpaste selects for ‐resistant within oral biofilm. Material Methods Dental plaque samples were collected from 40 individuals during year 5 randomised controlled trial. Participants had been randomly assigned use (3000 μg/ mL TCS) n = 18) or placebo 22). Diluted plated...
Dental caries is an endogenous microbial community-based disease resulting from ecological shift dynamic stability to metabolic imbalance in a consortium of acidogenic and aciduric bacteria comprising the dental plaque biofilm. Participants were members Dunedin Multidisciplinary Health Development Study, longitudinal investigation health behaviour cohort born Dunedin, New Zealand. Oral biofilm samples (collected at age 32 years) anterior labial supragingival, posterior lingual subgingival,...
This study assessed, for forensic purposes, the feasibility of genotypically matching oral streptococci recovered from recent human bite marks with those teeth biter.Streptococci were isolated incisors eight volunteers. Arbitrarily primed PCR (AP-PCR) distinguished 106 streptococcal genotypes among participants, each harbouring at least distinct strains. In a crime simulation, sample an experimental mark was analysed by experimenter unaware its origin. The bacteria unambiguously matched to...