A highly selective electrogenerated chemiluminescence (ECL) biosensor for the detection of target single-strand DNA (ss-DNA) was developed using hairpin as recognition element and ruthenium complex signal-producing compound. The ECL-based fabricated by self-assembling ECL probe thiolated tagged with on surface a gold electrode. In absence ss-DNA, immobilized electrode in folded configuration which its termini were held close proximity to electrode, thus strong signal could be generated....

A novel electrogenerated chemiluminescence peptide-based biosensor (ECL-PB) for the determination of prostate-specific antigen (PSA) was developed on basis target-induced cleavage a specific peptide within Nafion film incorporated with gold nanoparticles (AuNPs) and ECL emitting species. (CHSSKLQK) used as molecular recognition element; tris(2,2'-ripyridine) dichlororuthenium(II) (Ru(bpy)3(2+)) species, ferrocene carboxylic acid (Fc) employed quencher. The ECL-PB fabricated by casting...

Exploring new electrochemiluminescence (ECL) luminophores with high ECL efficiency and good stability in aqueous solution is great demand for biological sensing. In this work, highly efficient aggregation-induced enhanced of cyanophenyl-functionalized tetraphenylethene (tetra[4-(4-cyanophenyl)phenyl]ethene, TCPPE) its application biothiols analysis were reported. TCPPE contains four 4-cyanophenyl groups covalently attached to the (TPE) core, generating a nonplanar three-dimensional twisted...

Electrogenerated chemiluminescence (also called electrochemiluminescence and abbreviated ECL) involves the generation of species at electrode surfaces that then undergo electron-transfer reactions to form excited states emit light. ECL biosensor, combining advantages offered by selectivity biological recognition elements sensitivity technique, is a powerful device for ultrasensitive biomolecule detection quantification. Nanomaterials are considerable interest in biosensor field owing their...

A double covalent coupling method for the fabrication of a highly sensitive and reusable electrogenerated chemiluminescence (ECL) chemical sensor detection tertiary amines ECL aptamer-based (ECL-AB) biosensor cocaine is reported. The sensors were constructed by amino-containing Ru(bpy)(3)(2+) derivatives (Ru1, = tris(2,2'-bipyridyl)ruthenium(II)) or aptamer-Ru1 to surface paraffin-impregnated graphite electrode that had been covalently modified with monolayer 4-aminobenzene sulfonic acid via...

A simple electrochemiluminescence (ECL) immunoassay based on a proximity hybridization-regulated strategy was developed for highly sensitive and specific detection of cell surface protein protein-overexpressing cancer cells. biosensor fabricated by self-assembling thiolated capture ss-DNA3 (partially hybridize with ss-DNA1 ss-DNA2) blocking 6-mercapto-1-hexanol gold electrode surface. Target simultaneously bound two ss-DNA-tagged antibody probes (DNA1-Ab1 DNA2-Ab2), while DNA1 DNA2 were...

Abstract A signal-off impedimetric immune-biosensor based on gold nanoparticle (AuNP)-mediated electron transfer (ET) across a self-assembled monolayer (SAM) was the developed for highly sensitive detection of Escherichia coli O157:H7 bacteria. The biosensor fabricated by covalently grafting an anti- antibody onto SAM-modified electrodes. Following bacterial capture, sensor further modified nanoparticles (AuNPs). Due to strong interaction between AuNPs and O157:H7, attached surface bacteria...

In this work, a simple electrochemiluminescence (ECL) imaging method based on the cell shield of ECL emission was developed for morphological and quantitative analysis living cells under external stimulation. images MCF-7 cultured or captured at glassy carbon electrode (GCE) surface in solution tris(2,2′-bipyridyl)ruthenium(II)–tri-n-propylamine were recorded. Important characteristics cells, including shape, area, average boundary, junction distance between two adjacent directly obtained...

Organic nanoparticles (NPs) from donor-acceptor based coumarin derivatives, 6-[4-(N,N-diphenylamino)phenyl]-3-ethoxycarbonyl (DPA-CM), with an average size of 5.82 nm, were synthesized by a facile reprecipitation method using water as poor solvent and tetrahydrofuran good solvent. Red-shifted absorption, blue-shifted photoluminescence emission, aggregation-induced enhanced electrochemiluminescence (ECL) emission observed for the DPA-CM NPs in aqueous solution compared original organic...

8-Hydroxy-2′-deoxyguanosine (8-OHdG) is a typical biomarker of oxidative DNA damage and has attracted much attention in recent years since the level 8-OHdG body fluids typically associated with various diseases. In this work, simple highly sensitive electrochemical sensor for determination was fabricated incorporating single wall carbon nanotubes- (SWCNTs-) Nafion composite film coated on glassy electrode. chosen as an optimal adhesive agent from series agents acted binder, enrichment,...

An ATP-responsive and fluorescent Zeolitic imidazolate framework-8 (ZIF-8) is synthesized for real-time monitoring mitochondrial ATP fluctuation in living cells during photodynamic therapy.

The development of chronically implanted electrodes attracts much attention since these are favorable for monitoring changes neurotransmitters in brain science. research this field focused mainly on chemical modification to improve the potential stability and less biocompatibility. In work, first time, we proposed concept cell-membrane based a basic hypothesis using animal's self-cell membrane reduce animal exclusiveness (hyperacute rejection chronic rejection). As proof concept, studied...