A5020043146- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Advanced biosensing and bioanalysis techniques
- Plant Pathogenic Bacteria Studies
- Antibiotic Resistance in Bacteria
- Cancer Research and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Genetics, Bioinformatics, and Biomedical Research
- Bacterial biofilms and quorum sensing
- Molecular Communication and Nanonetworks
- Machine Learning in Materials Science
- Identification and Quantification in Food
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- bioluminescence and chemiluminescence research
- Biotin and Related Studies
- Advanced Biosensing Techniques and Applications
Institut Scientifique de Santé Publique
2013-2018
KU Leuven
2013-2016
Ghent University
2015-2016
Surveillance of Salmonella enterica subsp. serovar Typhimurium (S. Typhimurium) is generally considered to benefit from molecular techniques like multiple-locus variable-number tandem repeats analysis (MLVA), which allow earlier detection and confinement outbreaks. Here, a surveillance study, including phage typing, antimicrobial susceptibility testing the in Europe most commonly used 5-loci MLVA on 1,420 S. isolates collected between 2010 2012 Belgium, was evaluate added value for public...
Introduction: In April and May 2014, two suspected egg-related outbreaks of Salmonella enterica subsp.enterica serovar Enteritidis (S.Enteritidis) were investigated by the Belgian National Reference Laboratory Foodborne Outbreaks.Both food human isolates being available, this for both outbreaks, made these ideal case study a retrospective whole genome sequencing (WGS) analysis with goal to investigate feasibility technology outbreak investigation or Centre without thorough bioinformatics...
Whole genome sequencing represents a promising new technology for subtyping of bacterial pathogens. Besides the technological advances which have pushed approach forward, last years been marked by considerable evolution whole data analysis methods. Prior to application as routine epidemiological typing tool, however, reliable and efficient strategies need be identified among wide variety emerged methodologies. In this work, we compared three existing SNP-based workflows using benchmark...
This paper announces the genome sequence and annotation of genetically modified (GM) Bacillus subtilis strain 2014-3557 overproducing riboflavin (vitamin B2). GM-strain is unauthorized in European Union. Nevertheless, it has been isolated from a lot vitamin B2 (riboflavin) 80% feed grade imported to Europe China.
ObjectivesThe objective of this study was to design and validate a genotyping method for multiplex identification ESBLs carbapenemases in Gram-negative bacilli. This assay had be (i) superior traditional (multiplex) PCR/sequencing-based tests turn-around time, gene coverage the ability detect multiple variants same allele, (ii) significantly more cost-effective than commercial microarrays WGS. The targeted β-lactamases include (CTX-M families subtypes, ESBL non-ESBL SHV- TEM-likes,...
With multiplex oligonucleotide ligation-PCR (MOL-PCR) different molecular markers can be simultaneously analysed in a single assay and high levels of multiplexing achieved high-throughput format. As such, MOL-PCR is convenient solution for microbial detection identification assays where many should analysed, including routine further characterisation an identified pathogenic isolate. For aimed at use, optimisation terms differentiation between positive negative results cost effort...
Subtyping below the serovar level is essential for surveillance and outbreak detection investigation of Salmonella enterica subsp. Typhimurium (S. Typhimurium) its monophasic variant 1,4,[5],12:i:- 1,4,[5],12:i:-), frequent causes foodborne infections. In an attempt to overcome intrinsic shortcomings currently used subtyping techniques, a multiplex oligonucleotide ligation-PCR (MOL-PCR) assay was developed which combines different types molecular markers in high-throughput microsphere...
Abstract Characterization of microbial pathogens is necessary for surveillance, outbreak detection, and tracing sources. This unit describes a multiplex oligonucleotide ligation‐PCR (MOL‐PCR) optimized characterization pathogens. With MOL‐PCR, different types markers, like unique sequences, single‐nucleotide polymorphisms (SNPs) indels, can be simultaneously analyzed in one assay. assay consists ligation detection the singleplex PCR signal amplification, hybridization to MagPlex‐TAG beads...