A5043226983- SARS-CoV-2 detection and testing
- Gut microbiota and health
- Anaerobic Digestion and Biogas Production
- Microbial Metabolic Engineering and Bioproduction
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Microbial Fuel Cells and Bioremediation
- Gene expression and cancer classification
- Viral gastroenteritis research and epidemiology
- Wastewater Treatment and Nitrogen Removal
- Metabolomics and Mass Spectrometry Studies
- Biosensors and Analytical Detection
University of California, Berkeley
2024
Columbia University
2023
Sequencing human viruses in wastewater is challenging due to their low abundance compared the total microbial background. This study impact of four virus concentration/extraction methods (Innovaprep, Nanotrap, Promega, and Solids extraction) on probe-capture enrichment for followed by sequencing. Different yielded distinct profiles. Innovaprep ultrafiltration (following solids removal) had highest sequencing sensitivity richness, resulting successful assembly several near-complete genomes....
Until recently, wastewater-based monitoring for pathogens of public health concern primarily used PCR-based quantification methods and targeted sequencing specific (e.g., SARS-CoV-2). In the past three years, researchers have expanded to monitor a broad range pathogens, applying probe capture enrichment wastewater. The goals those studies included (1) expanding fundamental knowledge disease dynamics known (2) evaluating potential early detection emerging diseases resulting from zoonotic...
Abstract Sequencing human viruses in wastewater is challenging due to their low abundance compared the total microbial background. This study impact of four virus concentration/extraction methods (Innovaprep, Nanotrap, Promega, Solids extraction) on probe-capture enrichment for followed by sequencing. Different yielded distinct profiles. Innovaprep ultrafiltration (following solids removal) had highest sequencing sensitivity and richness, resulting successful assembly most near-complete...
Wastewater monitoring is rapidly expanding to provide surveillance for a growing number of epidemic and endemic pathogens. To early warning support rapid response novel virus through wastewater surveillance, it would be ideal understand in advance which concentration extraction methods are likely effective dPCR-based methods, depending on characteristics. In this study, we spiked samples with eight human respiratory viruses processed them four commercial that concentrate and/or extract...
Abstract In this study, a reduced total volatile fatty acid (VFA) yield (VFA/tCOD, P = .003) and accumulated fractions of butyric (BA) (BA/total VFA, < 0.001) was observed after feeding the arrested anaerobic digestor with thermal hydrolysis pretreated food waste. Further analysis through meta-omics revealed reduction in diversity acidification communities (Shannon index, .03), indicating sterilization effect THP on indigenous microbes feedstock. Additionally, although both...
Abstract In this study, two arrested anaerobic digestion bioreactors fed with food waste operated under different hydraulic retention times (HRTs) exhibited long-term stable volatile fatty acid (VFA) production performance including similar total yields ( p = .085) propionic (PA) being the most abundant VFA. Meta-omics analysis revealed distinct microbial structures .02) at HRTs while there were no differences in potential and extant functionality as indicated by whole-genome...