A5102830663- Chromosomal and Genetic Variations
- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Plant Disease Resistance and Genetics
- Ferroptosis and cancer prognosis
- Advanced Proteomics Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Biotin and Related Studies
Guangzhou Medical University
2024
Institute of Biophysics
2018-2021
Chinese Academy of Sciences
2018-2021
University of Chinese Academy of Sciences
2019
Abstract Protein–RNA interaction networks are essential to understand gene regulation control. Identifying binding sites of RNA-binding proteins (RBPs) by the UV-crosslinking and immunoprecipitation (CLIP) represents one most powerful methods map protein–RNA interactions in vivo. However, traditional CLIP protocol is technically challenging, which requires radioactive labeling suffers from material loss during PAGE-membrane transfer procedures. Here we introduce a super-efficient method...
Abstract The repression of transposons by the Piwi-interacting RNA (piRNA) pathway is essential to protect animal germ cells. In Drosophila ovaries, Panoramix (Panx) enforces transcriptional silencing binding target-engaged Piwi-piRNA complex, although precise mechanisms which this occurs remain elusive. Here, we show that Panx functions together with a germline specific paralogue nuclear export factor, dNxf2, and its cofactor dNxt1 (p15), as ternary complex suppress transposon expression....
Single-cell RNA sequencing (scRNA-seq) has transformed our understanding of cellular diversity with unprecedented resolution. However, many current methods are limited in capturing full-length transcripts and discerning strand orientation. We present RAG-seq, an innovative strand-specific total technique that combines not-so-random (NSR) primers Tn5 transposase-mediated tagmentation. RAG-seq overcomes previous limitations by delivering comprehensive transcript coverage maintaining...