A5103010878- Biocrusts and Microbial Ecology
- Microbial Community Ecology and Physiology
- Advanced Topics in Algebra
- Plant Reproductive Biology
- Rings, Modules, and Algebras
- Plant Molecular Biology Research
- Mycorrhizal Fungi and Plant Interactions
- Aquatic Ecosystems and Phytoplankton Dynamics
- Finite Group Theory Research
- Soil Carbon and Nitrogen Dynamics
- Photosynthetic Processes and Mechanisms
- Phytochemical compounds biological activities
- Invertebrate Immune Response Mechanisms
- Insect Resistance and Genetics
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Polyamine Metabolism and Applications
- Natural product bioactivities and synthesis
- Advanced Proteomics Techniques and Applications
- Groundwater and Isotope Geochemistry
- Statistical and numerical algorithms
- Mine drainage and remediation techniques
- Microencapsulation and Drying Processes
- Precipitation Measurement and Analysis
- Antimicrobial Peptides and Activities
University of Chinese Academy of Sciences
2018-2024
Institute of Tibetan Plateau Research
2017-2024
Chinese Academy of Sciences
2003-2024
Shanxi Normal University
2021-2024
Southern University of Science and Technology
2024
China Agricultural University
2021-2024
Anhui Agricultural University
2014-2020
Institute of Biophysics
2019
Center for Agricultural Resources Research
2018
Institute of Genetics and Developmental Biology
2018
Snake venom is a complex mixture of proteins and peptides, number studies have described the biological properties several venomous proteins. Nevertheless, complete proteomic profile from any many species snake not available. Proteomics now makes it possible to globally identify mixture. To assess profiles Naja naja atra Agkistrodon halys, snakes common southern China, we used combination strategy, which included following four different approaches: (i) shotgun digestion plus HPLC with...
An improved method for sample preparation MALDI-MS and MS/MS using AnchorChip targets is presented. The method, termed the SMW (sample, matrix wash), results in better sensitivity peptide mass fingerprinting as well sequencing by than previously published methods. allows up-concentration desalting directly on spectrometric target should be amenable automation. A draw back caused extensive oxidation of methionine tryptophan can alleviated addition n-octyl glucopyranoside DTT to solution. was...
Of the numerous factors affecting rice yield, how solar radiation is transformed into biomass through leaves most important. We have analyzed proteomic changes in collected from six different developing stages (vegetative to ripening). studied protein expression profiles of by running two-dimensional gel electrophoresis. Differential among phases were image analysis, which allowed identification 49 significantly spots. The spots further verified matrix-assisted laser...
Abstract Callus differentiation is a key developmental process for rice regeneration from cells. To better understand this complex process, we used 2‐D gel electrophoresis approach to explore the temporal patterns of protein expression at early stages during callus differentiation. This global analysis detected 60 known proteins out 79 spots identified by MS/MS, which many had been shown play role in plant development. Two new were revealed be associated with and have confirmed Western blot...
Abstract Thermoanaerobacter tengcongensis , one of many thermophilic organisms, survives harsh living conditions in temperatures ranging from 50 to 80°C. In this comprehensive analysis, we present a robust approach, 2‐DE and MALDI‐TOF MS, compare identify the bacterial proteins responding temperature stress. total, 164 spots were found with significant changes spot volume at three culture temperatures, 55, 75, 80°C, respectively; furthermore, 87 unique characterized by MS. Our results reveal...
Abstract Eukaryotic algae, as the primary group of photosynthetic autotrophs, exert a significant influence on development and functions biological crusts in dryland ecosystems. Despite their importance, there are substantial knowledge gaps composition eukaryotic algal communities effects distributions bacteria fungi soils. This study examined community along successional sequence biocrusts Gurbantunggut desert, while also investigating patterns co‐occurrence with through high‐throughput...
A fundamental issue for sepsis therapy is to control the development of inflammation at an early stage. With cecal ligation and puncture (CLP) surgery, mouse model has clearly shown septic signs triggered by chronic insult. To monitor plasma proteomic responses sepsis, blood was collected intervals after sham CLP surgery followed sample treatment remove high abundance serum albumin. The treated proteins were well resolved two-dimensional electrophoresis (2-DE). image analysis revealed that...
Abstract DNA methylation is one of the most widespread epigenetic marks and has been linked to insect development, especially influencing embryonic development. However, regulation in silkworm development diapause remain investigate. In this study, reverse‐transcription quantitative polymerase chain reaction was performed identify expression level Bombyx mori methyltransferases (BmDNMTs) 1 2 ( BmDnmt1 BmDnmt2 ) different tissues, developmental stages, strains silkworm. The results showed...
Abstract The definition of dominance or epistasis is generally on the basis a descriptive characterization for these crops in field, such as yield per hectare and weight grain. Since trait examinations lack molecular information, how to precisely predict phenotypic changes filial generation still problem heterosis studies. For rice, genetic information caused by hybridization can be archived through analyzing proteomes rice seeds. Differential analysis was introduced seeds three cultivars,...
Three novel oligopeptides, asterinin A–C have been isolated from the roots of A. tataricus and their structures elucidated by chemical, enzymatic spectral methods.
Abstract Known as “China's Water Tower,” the Qinghai–Tibet Plateau (QTP) becomes warm and humid in short term but faces drought long because of global climate change. Atmospheric water resources seem to be only choice QTP region alleviate conditions. Analysis vapour distribution evaluation exploitation modes atmospheric are carried out this study. Different including routine, selective, emergency‐oriented distinguished using budget its entropy. Zoning indicates that routine exploitation, is,...
Symbiotic diazotrophs form associations with legumes and substantially fix nitrogen into soils. However, grasslands on the Qinghai-Tibet Plateau are dominated by non-legume plants, such as Kobresia tibetica . Herein, we investigated diazotrophic abundance, composition, community structure in soils roots of three K. humilis legume Oxytropis ochrocephala , using molecular methods targeting nifH gene. Diazotrophs were abundantly observed both bulk rhizosphere soils, well all but their abundance...
Abstract The repression of transposons by the Piwi-interacting RNA (piRNA) pathway is essential to protect animal germ cells. In Drosophila ovaries, Panoramix (Panx) enforces transcriptional silencing binding target-engaged Piwi-piRNA complex, although precise mechanisms which this occurs remain elusive. Here, we show that Panx functions together with a germline specific paralogue nuclear export factor, dNxf2, and its cofactor dNxt1 (p15), as ternary complex suppress transposon expression....