Abstract Background Plastic waste accumulation is one of the main ecological concerns in past decades. A new generation plastics that are easier to degrade environment compared conventional plastics, such as starch-based bioplastics and oxo-biodegradable perceived a solution this issue. However, fate these materials unclear, less known about how their presence affect microorganisms may play role biodegradation. In study, we monitored dynamics bacterial community soil upon introduction...

Tempe is an Indonesian fermented food prepared by fermenting dehulled cooked soybeans with Rhizopus oligosporus. Many types of bacteria are also involved during tempe fermentation, and one these Klebsiella spp. Some isolates K. pneumoniae produces vitamin B12 in but it has been classified as opportunistic pathogen. For this reason important to be studied. The aim study was investigate the genetic diversity from employing ERIC-PCR method. Sixty-one have isolated sixteen producers Bogor,...

Amplicon sequencing targeting 16S ribosomal RNA (rRNA) has been widely used to profile the microbial community from fermented food samples. However, polymerase chain reaction (PCR) steps on amplicon analysis and intragenomic heterogeneity within rRNA are believed contribute bias in estimating composition. As potential paraprobiotics sources, a comprehensive profiling study of tempeh ecology could product development. This employed shotgun metagenomic approach, where metagenome fragments...

Soaking is one of the important steps in tempeh production. Various microorganisms grow during soaking process, which can affect nutrition and quality tempeh. This study aimed to determine effect adding lactic acid bacteria (LAB) process determined preference levels produced In this research, Lactobacillus plantarum HPADA3 fermentum HPBD2 were added soybeans. Each LAB had been evaluated for its hemolytic activity potential virulence factor genes. The duration was much shorter than commonly...

Foodborne disease and food spoilage are the prime public health issue security round globe. Significant outbreaks mostly linked to existence of pathogenic bacteria that extremely challenging due persistence biofilm-forming. Proteins bacterial metabolites have been shown good antibacterial activity effectively removal biofilm. Recently, bacteriophage their encoded lytic proteins such as lysin attracted attention potential alternative agent control undesirable pathogens in human body...

Fermented cassava or "Tape" is one of traditional Indonesian fermented food. The quality determined by microorganisms involved during fermentation process. It was reported that Bacillus subtilis the "Tape". most common way to identify species using 16S rRNA gene. This gene contains conserved regions as unique sequence which relative among species. has been widely used a reliable molecular marker for phylogeny identification. Therefore, aim this research study diversity amylase-producing spp....

Klebsiella pneumoniae strain IIEMP-3, isolated from Indonesian tempeh, is a vitamin B12-producing that exhibited different genetic profile pathogenic isolates. Here we report the draft genome sequence of which may provide insights on nature fermentation, nutrition, and immunological function tempeh.

Aims: Our previous study demonstrated that Klebsiella IIEMP-3 associated with tempeh was genetically different from those of medical isolates.In addition to the whole genome sequence IIEMP-3, draft another isolate, i.e.IWJB-6 employed for comparison.In this study, details virulence genes and unique gene in both isolates were compared employing silico vitro analysis.Methodology results: Whole IWJB-6 annotated investigate factor.Klebsiella obtained producers Bogor, West Java -Indonesia.Genome...

The high application of Poly(styrene-maleic acid) (PSMA) in an aqueous environment, such as biomedical purposes, makes the interaction between PSMA and water molecules interesting to be investigated. This study evaluated conformation, hydrogen bond network, stabilities all possible intermolecular interactions with (PSMA−(H2O)n, n = 1–5). All calculations were executed using density functional theory (DFT) method at B3LYP 6–311G** basis set. energy PSMA–(H2O)5 complex was –56.66 kcal/mol,...

Screening of proteolytic and fibrinolytic bacteria from Indonesian soy bean based fermented food Oncom revealed several potential isolates. Based on 16s rDNA gene analysis, one particular isolate with the highest activity was identified as Stenotrophomonas sp. The protease amplified to generate a 1749 bp Polymerase Chain Reaction product BLAST 90% homology encoding enzyme maltophilia. putative amino acid sequence indicated serine typical aspartate, histidine in catalytic triad. translated...

Several in vitro studies have focused on the ability of artemisinin and its derivatives to suppress lung cancer cells, but there are still many biological processes mechanisms, such as interactions between active compounds (ligands) receptors that not well understood require further research. Drug targeting for will be more precise inhibition cell proliferation increased a result understanding mechanism interaction with receptors. Currently, no report PARP1 receptor. The results this study...

DLBS2411, a bioactive fraction of Cinnamomum burmannii, has been proven to exhibit its anti-ulcer properties through preclinical and clinical studies. However, the compounds, protein targets, underlying molecular pathways are still poorly known. Therefore, network pharmacology was used in this study understand mechanisms DLBS2411 treating gastritis. There were three stages investigation. First, compounds targeted proteins associated with gastritis gathered examined. The subsequent stage...

Penyakit bawaan pangan masih merupakan masalah penting di negara berkembang termasuk Indonesia. ini berdampak terhadap keamanan serta kesehatan masyarakat. Diare penyebab mortalitas tertinggi diantara berbagai penyakit lain untuk anak-anak bawah usia lima tahun. Kontrol dapat dilakukan melalui peningkatan sanitasi gizi dalam kehidupan sehari-hari. Namun sayangnya, pengetahuan yang terbatas dari para ibu rumah tangga tentang bagaimana menyiapkan aman dan bergizi berkontribusi besar...

This research focuses on the characterization of four mannanase enzymes regarding their structure and stability across various pH conditions. The study employed in silico techniques, including homology modelling, protein superimposition, protonation state assignment, molecular dynamics simulations, to analyze enzyme's behaviour. results revealed distinct characteristics for each enzyme, suggesting potential different industrial applications. Furthermore, identified mutations involving...

Inflammatory markers such as cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), myeloperoxidase (MPO), and prostaglandin (PEG) are widely known major targets in discovering natural anti-inflammatory drugs for the treatment of inflammationrelated diseases. Non-steroidal (NSAIDs) ibuprofen aspirin mostly used at present, however, some NSAIDS have been reported to cause gastrointestinal side effect due ligand-protein interaction. Molecular docking is a promising tool study modes In...

The rhizosphere is a narrow soil area directly affected by plant root exudates. Microbes inhabiting the have been widely studied for their beneficial effects on nutrition, growth, and disease prevention. Many factors affect microbial composition, including pathogen infection. Here, we analyzed bacterial community structure in of fungi-infected Amorphophallus titanum. Soil samples were collected from non-rhizosphere areas A. 16S metagenomic analysis was conducted to investigate amplifying...

Hepatoprotective Activity of Methanolic Extract Rhyncosia Beddomei Baker Leaves Against Carbon Tetrachloride Induced HepatotoxicityAshoka Babu V.L, Arunachalam G, Jayaveera K.N, Madhavan.V, Shanaz Banu

Abstract Background: Plastic waste accumulation is one of the main ecological concerns in past decades. A new generation plastics that are easier to degrade environment compared conventional plastics, such as starch-based bioplastic and oxo-degradable plastic, perceived a solution this issue. However, degraders materials their fate unclear. In study, we monitored dynamics bacterial community soil upon introduction commercial plastic. The were buried separately compost incubated for 30, 60,...

Soybeans tempeh (tempeh) is processed by fermentation using Rhizopus spp. Tempeh an important source of protein in Indonesia. The traditional inoculum locally known as Usar which made from the leaves Hibiscus tiliaceus. However, information still limited. Therefore, this study aims to identify and investigate genetic diversity species based on Internal Transcribed Spacer (ITS) sequence Random Amplified Polymorphic DNA (RAPD) markers. Twenty-three strains were isolated ten tempeh. Based ITS...

Tempe bungkus daun jati merupakan produk pangan fermentasi tradisional Indonesia yang kaya protein, serat, dan vitamin sangat popular di Kabupaten Blora, Jawa Tengah. Penelitian ini bertujuan mengetahui pengaruh penambahan kelor pada tahapan perendaman peragian dalam proses produksi tempe terhadap profil bakteri asam laktat (BAL) tingkat kesukaan. Daun segar serbuk disuplementasikan dengan variasi konsentrasi kacang kedelai. Hasil penelitian menunjukkan bahwa tidak menghambat aktivitas...

Oil palm (Elaeis guineensis) is one of the most important oil-bearing crop in world. This can be vegetatively propagated only using tissue culture technique. technique has low efficiency, with callogenesis and embryogenesis stages as limiting factors. Genetic factor a major role determining success rate these two stages. The use molecular markers which represent or potential to improve efficiency oil process. In this study, SNP mining was conducted on transcriptome data, cDNA database,...

Abstract Objective Amplicon sequencing targeted 16S ribosomal RNA (rRNA) has been widely used for the analysis profile of microbial community from fermented food samples. Previous results rRNA metagenome showed that Firmicutes was dominant phylum in tempeh. However, polymerase chain reaction (PCR) steps on amplicon and intragenomic heterogeneity within are believed to contribute bias estimation composition. An alternative approach known as shotgun metagenomic might be able avoid this...