A5031327909- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Electrowetting and Microfluidic Technologies
- 3D Printing in Biomedical Research
- Nanopore and Nanochannel Transport Studies
- Neuroscience and Neural Engineering
- Electrohydrodynamics and Fluid Dynamics
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Cell Image Analysis Techniques
- RNA and protein synthesis mechanisms
- Electrostatics and Colloid Interactions
- RNA regulation and disease
- Analytical Chemistry and Sensors
- Digital Holography and Microscopy
- Microbial Inactivation Methods
- Electrical and Bioimpedance Tomography
- Biosensors and Analytical Detection
- Single-cell and spatial transcriptomics
- Graphene research and applications
- Monoclonal and Polyclonal Antibodies Research
- Cellular Mechanics and Interactions
- Graphene and Nanomaterials Applications
- Peer-to-Peer Network Technologies
- Advanced Biosensing Techniques and Applications
Beijing University of Chemical Technology
2018-2024
Northwest A&F University
2023-2024
Hong Kong University of Science and Technology
2012-2019
University of Hong Kong
2014-2019
Shanghai Jiao Tong University
2011
Beijing Institute of Technology
2008
Abstract A-to-I mRNA editing in animals is mediated by ADARs, but the mechanism underlying sexual stage-specific fungi remains unknown. Here, we show that eukaryotic tRNA-specific heterodimeric deaminase FgTad2-FgTad3 responsible for Fusarium graminearum . This capacity relies on interaction between FgTad3 and a protein called Ame1. Although Ame1 orthologs are widely distributed fungi, originates Sordariomycetes. We have identified key residues FgTad3-Ame1 interaction. The expression...
A facile single-cell patterning (ScP) method was developed and integrated with time-of-flight secondary ion mass spectrometry (TOF-SIMS) for the study of drug-induced cellular phenotypic alterations. Micropatterned poly(dimethylsiloxane) (PDMS) stencil film centrifugation-assisted cell trapping were combined preparation on-surface microarrays, which exhibited both high site occupancy (>90%) resolution (>97%). TOF-SIMS is a surface-sensitive increasingly utilized in biological studies. Here...
Abstract SIMIT-seq is a bead-free, scalable microfluidic platform designed for high-efficiency single-cell mRNA sequencing. Conventional microfluidic-based RNA sequencing platforms rely heavily on barcoded beads and intricate co-encapsulation schemes, often constrained by double Poisson limitations the complexities of bead synthesis. In contrast, eliminates need entirely employing deterministic, orthogonal barcoding strategy within two-dimensional micro-well array. This achieves an...
Chemotherapeutic drugs such as paclitaxel and vinblastine interact with microtubules thus induce complex cell states of mitosis arrest at the G2/M phase followed by apoptosis dependent on drug exposure time concentration. Microfluidic impedance cytometry (MIC), a label-free high-throughput technology for single-cell analysis, has been applied viability assay cancer cells post fixed dosage, yet verification this technique varied tumor after anticancer treatment remains challenge. Here we...
Continuous-flow cell sorting at considerably high flow rates by railing cells along electrode tracks based on dielectrophoresis and hydrodynamic drag.
Mitosis is a crucial biological process where parental cell undergoes precisely controlled functional phases and divides into two daughter cells. Some drugs can inhibit mitosis, for instance, the anti-cancer interacting with tumor proliferation leading to mitosis arrest at specific phase or death eventually. Combining machine learning microfluidic impedance flow cytometry (IFC) offers concise way label-free high-throughput classification of drug-treated cells single-cell level. IFC-based...
We demonstrate 3D microelectrodes featuring castellated blocks to facilitate dielectrophoretic trapping of cells near stagnation points for enhanced cell viability.
Microelectrodes and flow sidewalls entirely made of AgPDMS by one-step replica molding process for dielectrophoretic cell separation viability assay.
This paper describes the design, fabrication, and characterization of an interdigitated 3-D silicon (Si) ring microelectrodes for dielectrophoretic (DEP) manipulation particles. The are derived from a high-aspect-ratio comb structure etched in doped single crystal Si on insulating dielectric (silicon-on-insulator). Fingers evolved into once perforated with linear array well-defined round lateral constrictions. is achieved by segmented finger layout dry release strategy borrowed inertial...
Mechanically deforming biological cells through microfluidic constrictions is a recently introduced technique for the intracellular delivery of macromolecules possibly transient membrane pores induced in process. The attractive research and clinical applications mainly because it simple, fast, effective while being free adverse effects often associated with well-known techniques that rely on field- or vector-based delivery. In this nascent approach, an utmost crucial role played by...
We present a novel plasmapheresis device designed for fully integrated point-of-care blood analysis microsystem. In the device, fluidic microchannels exhibit characteristic cross-sectional profile arising from distinct three-dimensional (3D) microelectrodes featuring sidewall undercuts readily through single-mask process. The structure leverages mainly electrothermal convective rolls that efficiently manifest themselves in physiological fluids and yet have received inadequate attention...
Abstract Although genome-wide A-to-I editing mediated by adenosine-deaminase-acting-on-tRNA (ADAT) occurs during sexual reproduction in the presence of stage-specific cofactors, RNA is not known to occur vegetative growth filamentous fungi. Here we identified 33 events hyphae Fusarium graminearum and functionally characterized one conserved hyphal-editing site. Similar ADAT-mediated reproduction, majority sites are coding sequences nonsynonymous, have strong preference for U at -1 position...
Highly localized (point) constrictions featuring a round geometry with ultra-sharp edges in silicon have been demonstrated for the reagent-free continuous-flow rapid mechanical lysis of mammalian cells on single-cell basis. Silicon point constrictions, robust structures formed by single-step dry etching process, are arranged cascade along microfluidic channels and can effectively rupture delivered pressure-driven flow. The influence constriction size count performance is presented...
Abstract A-to-I mRNA editing occurs during fungal sexual reproduction with an unknown mechanism. Here, we demonstrated that the eukaryotic tRNA-specific heterodimeric deaminase FgTad2-FgTad3, not typically associated editing, is responsible for in Fusarium graminearum . This capacity relies on interaction between FgTad3 and a stage-specific protein called Ame1. The emerged Sordariomycetes. Key residues involved have been identified. Expression activity of FgTad2-FgTad3 are regulated through...
Bioelectronics, an emerging discipline formed by the biology and electronic information disciplines, has maintained a state of rapid development since its birth. Amongst various functional bioelectronics materials, bacteriorhodopsin (bR), with directional proton pump function favorable structural stability properties, drawn wide attention. The main contents paper are as follows: Inspired capacitive properties natural protoplast cell membranes, new bio-capacitor based on bR artificial...
This work for the first time uses silver PDMS (AgPDMS) composites to construct 3D microelectrodes featuring non-uniform sidewall profile. Such design enables an innovative dielectrophoretic (DEP) cell sorting device with electrodes and fluidic structures being molded simultaneously completely made of AgPDMS. The interdigitated electrode digits exhibit inhomogeneous two-layer structure, which projects highly electric field that separates cells not only laterally but also vertically along...
This work presents an innovative design for a flow-through dielectrophoretic cell sorting based on silicon bulk microelectrodes featuring sidewall undercuts. The are configured into interdigitated array with digits extending across the flow chamber at oblique angle against stream. Target cells under forces and hydrodynamic drag can slide along to dedicated outlet. has been showcased continuous-flow of viable non-viable mammalian cells, achieving throughput 16,600 cells/min, order magnitude...
We report the velocity measurement of microscopic particles flowing at ultrahigh speed with optofluidic time-stretch microscopy high throughput. This is a study using optical microcopy as tool for particle velocimetry, where we developed custom algorithm to process images acquired from platform calculation individual particles. experimentally determined actual flow velocities polystyrene microspheres different sizes and traveling through microchannel throughput ∼10 000 particles/s. also...
This paper presents an integrated nanofluidic diode biosensor based on a single asymmetric nanoslit with nominal width of 30 nm. The dimension is fine-tuned by the highly conformal atomic layer deposition (ALD) Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> . surface further modified ultra-thin SiO that placed onto for improved sensing performance. device has been demonstrated...
This is the first account of a single-cell impedance flow cytometry microsystem based on 3D silicon microelectrodes. Such microelectrodes emerge being readily aligned during lithographic patterning and thus relieve burden alignment that comes with planar their opposing arrangement for maximum sensitivity. The generate homogeneous electric field maintain sensitivity while individual cells passing by. has been showcased here measurement polystyrene microspheres at 10 MHz. Successful...
This work for the first time demonstrates a single-cell impedance micro-cytometer with 3D differential electrodes and fluidic sidewalls completely made of silver-PDMS composites (AgPDMS). Such monolithically integrated electro-fluidic structure is an outcome one-step molding from readily used master, obviating extra sacrificial layer patterning as required in existing device incorporating AgPDMS electrodes. To maintain maximum sensitivity, exploits sidewall electrode configured...