A5085589370- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
- Fish Ecology and Management Studies
- Wheat and Barley Genetics and Pathology
- Environmental Toxicology and Ecotoxicology
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Aquaculture disease management and microbiota
- Crop Yield and Soil Fertility
- Economic and Environmental Valuation
- Receptor Mechanisms and Signaling
- Turfgrass Adaptation and Management
- Environmental Sustainability in Business
- Water resources management and optimization
- Housing Market and Economics
- Neuropeptides and Animal Physiology
- Ion Transport and Channel Regulation
- Botany, Ecology, and Taxonomy Studies
- Fish Biology and Ecology Studies
- Environmental Education and Sustainability
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Neurobiology and Insect Physiology Research
- Water Governance and Infrastructure
Colorado State University
2008-2022
Okanagan University College
1995-2015
University of British Columbia
2001-2015
Okanagan College
1997-2011
University of Ottawa
1991-2011
University of Alberta
2003
McMaster University
1991-1998
University of Kentucky
1991
Adult and juvenile rainbow trout, Salmo gairdneri, acclimated to high low water Ca levels were statically exposed equimolor concentrations (~ 6.5 μmolùL −1 ) of Cd or Cu at circumneutral pH 4.8 either alone in metal/H + combinations for 24 h. Unidirectional fluxes 2+ Na measured by means radiotracers terminal blood samples drawn analysis . Plasma was found not be a reliable indicator disturbances branchial fluxes. Cadmium specifically inhibited influx, while having no effect on efflux only...
Carbohydrate and protein metabolism were examined in rainbow trout, Oncorhynchus mykiss, fitted with mini-osmotic pumps which maintained plasma cortisol levels at approximately 100 or 200 ng∙mL −1 for 10 d. Plasma glucose, lactate, unaffected by d of administration, despite a significant elevation cortisol. amino acids cortisol-treated fish (1023.8 ± 90.7 μg∙mL ) significantly elevated compared shams (716.7 68.5 after 9 Liver glycogen content was reduced treatment. The activities the liver...
A magnetic cell separation technique (MACS) was developed for isolating and characterizing peanut lectin agglutinin positive (PNA + ) cells from rainbow trout gills. Percoll density separated mitochondria-rich (MR) were serially labeled with PNA-FITC an anti-FITC antibody covalently coupled to a 50-nm iron particle then applied column. PNA MR enriched >95% purity. Transmission electron microscopy analysis of both the negative − fraction showed that binds chloride while is comprised...
Heading date in wheat (Triticum aestivum L.) and other small grain cereals is affected by the vernalization photoperiod pathways. The reduced-height loci also have an effect on growth development. date, which occurs just prior to anthesis, was evaluated a population of 299 hard winter entries representative U.S. Great Plains region, grown nine environments during 2011-2012 2012-2013. germplasm for candidate genes at (Vrn-A1, Vrn-B1, Vrn-D1), (Ppd-A1, Ppd-B1 Ppd-D1), (Rht-B1 Rht-D1) using...
Phenotypic plasticity describes the range of phenotypes produced by a single genotype in different environments. We quantified extent phenotypic (evaluated as responsiveness to varying environmental conditions) thermal time heading and grain yield 299 hard winter wheat ( Triticum aestivum L.) genotypes from US Great Plains. The genotypes, which included advanced breeding lines recent historic cultivars, were evaluated 11 environments 2011–2012 2012–2013. average number days 1 January across...
Previous work suggested that Ca2+ and Zn2+ share a common uptake pathway in rainbow trout gills. We here report on relationships between the kinetic variables for unidirectional influx during 1 month exposure of freshwater to (150 µg l-1=2.3 µmol l-1 as total zinc, Zn). Initial caused large competitive inhibition influx, indicated by threefold increase apparent Km (measured presence Zn2+). There was also smaller non-competitive (50 % decrease Jmax) transport system, which...
The objective of this study was to characterize the metal binding external gill surface in an attempt interrelate chemistry, activity, and possibly toxicity. Individual arches from adult rainbow trout (Oncorhynchus mykiss) were exposed various concentrations either 140 La, 45 Ca, 109 Cd, or 64 Cu. apparent dissociation constant (K D ) capacity (B max for metals calculated using Scatchard plot analysis. Half-saturation time (t 1/2 cooperativity (H n also determined. K ranged 0.31 ± 0.02 mM La...
Calcitonin gene-related peptide (CGRP), a vasoactive neuropeptide present in peripheral neurons, is released at local sites of inflammation. In these studies specific high affinity adenylyl cyclase linked CGRP receptors were characterized on rat lymphocytes. The distribution, affinity, and specificity was analyzed by radioligand binding. 125I-[His10]CGRP binding to lymphocytes rapid, reaching equilibrium 20 30 min 22 degrees C, dependent cell concentration. dissociation constants, Kd, for...
Core Ideas Great Plains winter wheat genotypes differed for root traits in a greenhouse study. Total length was negatively correlated with average diameter under moisture stress. Root deep the profile positively associated leaf relative water content. Semi‐dwarfing alleles did not significantly affect any system trait. Development of deep, extensive is drought adaptation mechanism that beneficial maintaining productivity scarcity dryland cropping systems. To determine variation biomass,...
Percoll density-gradient separation, combined with peanut lectin agglutinin (PNA) binding and magnetic bead was used to separate dispersed fish gill cells into sub-populations. Functional characterization of each the sub-populations performed determine which displayed acid-activated phenamil- bafilomycin-sensitive Na(+) uptake. Analysis mechanism(s) (22)Na(+) influx in control (addition 10 mmoll(-1) proprionic acid) using a variety transport inhibitors (ouabain, phenamil, HOE-694 bumetanide)...
Plasma catecholamine levels and arterial blood respiratory variables were monitored in rainbow trout (Oncorhynchus mykiss) American eel (Anguilla rostrata) acutely exposed (30 min) to graded of external hypoxia [water PO2 (PWO2) 20-90 Torr]. The experiments designed evaluate the factors controlling mobilization hypoxic fish elucidate basis marked interspecific differences. In trout, plasma unchanged when PWO2 remained above 50 Torr but increased markedly was lowered below this value;...
Hepatocyte beta-adrenoceptors were characterized in trout with chronically raised plasma cortisol levels (132.1 +/- 14.8 ng/ml, n = 8, 11-12 days) and compared shams (1.8 0.9 8) using radioreceptor assay techniques. Hepatocytes prepared from sham possessed 1,696 179 beta-adrenoceptors/cell. These receptors as beta 2-adrenoceptors based on the potency order of specific inhibition CGP binding. The number putative surface significantly increased to 6,005 1,165 receptors/cell, hepatocytes...
ABSTRACT We have investigated the influence of cortisol on β-adrenoreceptor population rainbow trout [Oncorhynchus mykiss (Walbaum)] erythrocytes and determined what impact it has adrenergic responsiveness in vitro to exogenous catecholamines. To do so, erythrocyte β-adrenoreceptors were characterized fish with chronically elevated plasma levels (118±5.9ngml−1, >10 days) compared shams, using radioreceptor assay techniques. The number ‘internalized’, low-affinity receptors was...
Juvenile rainbow trout (Oncorhynchus mykiss) were exposed for 21 d to one of three concentrations H + and Al. Exposure 27 μg Al∙L −1 at pH 5.2 resulted in a significant internal physiological disturbance associated with the accumulation Al gill, both which declined continued exposure. Increased resistance an acutely lethal concentration (118 μg∙L , 5.2) was demonstrated by reduction rate mortality magnitude relative controls 6.5 or 0 Both strong Ca 2+ antagonists causing modifications...
ABSTRACT Improved end‐use quality is an essential goal of many wheat ( Triticum aestivum L.) breeding programs. Besides genetic factors, environmental conditions are known to influence quality. Our objective was identify quantitative trait loci (QTL) for traits in a winter population under different soil moisture levels. A 185 doubled haploid (DH) lines derived from cross between hard white wheats CO940610 and ‘Platte’. The grown four environments (two limited irrigation two fully irrigated)...
Due to variable moisture conditions in the U.S. Great Plains, it is important understand genetic control of crop traits under a range soil levels. Our objective was identify quantitative trait loci (QTL) for yield, phenology, and morphological wheat (Triticum aestivum L.) different conditions. Field evaluation winter doubled haploid population (n = 185) derived from cross between CO940610 ‘Platte’ carried out Fort Collins Greeley, Colorado, USA 2007–2008 2008–2009, respectively. At each...
Des truites juveniles (Oncorhynchus mykiss) ont ete exposees a une simulation de rechauffement global et d'acidification l'eau pendant periode 90 jours en (plage temperature temoin 13-24°C). Le relevement 2°C du cycle fluctuant la estivale des eaux cotieres lac Ontario l'ajout H 2 SO 4 douce synthese servi pratiquer quatre traitements : temoin, acidification seulement, plus acidification. regime d'alimentation biquotidien fait monter le metabolisme 75% Mo (max). De fortes augmentations (de...
Intraspecific genetic variation may contribute significantly to invasiveness and control problems, but has been characterized date in relatively few invasive weed species. We examined 56 intersimple sequence repeat (ISSR) loci 220 individuals from 11 invading populations of yellow toadflax sampled across five western states. All showed high levels diversity. Estimated values for Shannon's diversity measure ranged 0.217 0.388, expected heterozygosity 0.178 0.260. Nei's total gene index ( H T...
This experiment examined the metabolic costs and physiological consequences of growth energetics juvenile rainbow trout Oncorhynchus mykiss in a warmer, more polluted winter environment. Growth under warm-winter conditions was approximately three times less than equivalent experimental control groups previously observed warm-summer conditions. However, during exposure, wet weights total lengths were roughly 30% higher “warmed” fish base temperature group due to combination greater appetite...