A5026580360- Escherichia coli research studies
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Carbohydrate Chemistry and Synthesis
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Viral gastroenteritis research and epidemiology
Stockholm University
2019-2022
Lund University
2019
Carbohydrate structure can be elucidated or confirmed by using NMR spectroscopy as the prime technique. Prediction of 1H and 13C chemical shifts computational approaches makes this assignment process more efficient program CASPER perform task rapidly. It does so relying on shift data mono-, di-, trisaccharides. In order to improve accuracy quality these predictions we have assigned 30 monosaccharides, 17 disaccharides, 10 trisaccharides one tetrasaccharide; in total 58 compounds. Due...
The structure of the O-antigen from Escherichia coli reference strain O188 (E. O188:H10) has been investigated. lipopolysaccharide shows a typical nonrandom modal chain-length distribution and sugar absolute configuration analysis revealed d-Man, d-Glc, d-GlcN d-GlcA as major components. O-specific polysaccharide was determined using one- two-dimensional 1H 13C NMR spectroscopy experiments, where inter-residue correlations were identified by 1H,13C-heteronuclear multiple-bond correlation...
Shigella flexneri is a major causative agent of bacillary dysentery in developing countries, where serotype 2a2 the prevalent strain. To date, approximately 30 serotypes have been identified for S. flexneri, and contribution to emergence new chemical modifications lipopolysaccharide (LPS) component O antigen (Oag). Glucosylation, O-acetylation, phosphoethanolamine (PEtN) increase Oag diversity, providing benefits LPS acts as primary receptor bacteriophage Sf6, which infects only limited...
Galectins are carbohydrate recognition proteins that bind carbohydrates containing galactose and involved in cell signaling cellular interactions, involving them several diseases. We present the synthesis of (aryltriazolyl)methyl galactopyranoside galectin inhibitors using a highly diastereoselective hydroboration C1-exo-methylene pyranosides giving with fourfold or better selectivity for galectin-1 over galectin-3, -4C (C-terminal CRD), -4N (N-terminal -7, -8C, -8N, -9C, -9N dissociation...
Abstract Enteropathogenic Escherichia coli O125, the cause of infectious diarrheal disease, is comprised two serogroups, viz., O125ab and O125ac, which display aggregative adherence pattern with epithelial cells. Herein, structure O-antigen polysaccharide from E. O125ac:H6 has been elucidated. Sugar analysis revealed presence fucose, mannose, galactose N-acetyl-galactosamine as major components. Unassigned 1H 13C NMR data one- two-dimensional experiments O125ac in conjunction sugar...
Abstract The structure of the O-antigen from international reference strain Escherichia coli O93:−:H16 has been determined. A nonrandom modal chain-length distribution was observed for lipopolysaccharide, a pattern which is typical when long O-specific polysaccharides are expressed. By combination (i) bioinformatics information on gene cluster related to synthesis including putative function glycosyl transferases, (ii) magnitude NMR coupling constants anomeric protons, and (iii) unassigned...
The structure of the O-antigen from international reference strain Escherichia coli O93:−:H16 has been determined. A nonrandom modal chain-length distribution was observed for lipopolysaccharide, a pattern which is typical when long O-specific polysaccharides are expressed. By combination (i) bioinformatics information on gene cluster related to synthesis including putative function glycosyl transferases, (ii) magnitude NMR coupling constants anomeric protons and (iii) unassigned 2D...