Chami Amarasinghe

ORCID: 0000-0002-0909-2605
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About
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Research Areas
  • Plant Pathogens and Fungal Diseases
  • Mycotoxins in Agriculture and Food
  • Wheat and Barley Genetics and Pathology
  • Plant Disease Resistance and Genetics
  • Yeasts and Rust Fungi Studies
  • Plant-Microbe Interactions and Immunity
  • Bioenergy crop production and management
  • Berry genetics and cultivation research
  • Plant Pathogenic Bacteria Studies
  • Plant Pathogens and Resistance

Agriculture Food and Rural Development
2024-2025

University of Manitoba
2011-2019

Abstract Fusarium head blight (FHB) continues to threaten the economic sustainability of many small grain producers in Manitoba by causing losses yield and quality. In this 2-year study, four fungicides were tested on two spring wheat cultivars with different levels resistance FHB inoculated 3ADON 15ADON chemotypes graminearum. All (prothioconazole, tebuconazole, metconazole prothioconazole+ tebuconazole) reduced index, per cent Fusarium-damaged kernels (% FDK) deoxynivalenol (DON) increased...

10.1080/07060661.2013.773942 article EN Canadian Journal of Plant Pathology 2013-02-07

Fusarium head blight (FHB), caused principally by the species belonging to graminearum complex (FGSC), is an important disease in wheat, barley, and other small grain crops worldwide. Grain infected with FGSC may be contaminated trichothecene mycotoxins such as deoxynivalenol (DON) nivalenol (NIV). In this study, we characterized phylogenetic relationships, chemotype diversity, phenotypic characters, aggressiveness of 150 strains collected from eight different countries. Phylogenetic...

10.3390/toxins11050263 article EN cc-by Toxins 2019-05-11

Wheat leaf rust, caused by Puccinia triticina Erikss., is one of the most common and damaging diseases wheat in Canada throughout world. To understand P. population virulence analysis has been conducted annually for over 80 years. The profile to key resistance genes changes significantly time, differs between regions. Recently, genetic via DNA sequencing representative isolate from populations 2018-2022 initially revealed three diverse groups Canada. All isolates within group had same two...

10.1139/cjps-2024-0151 article EN Canadian Journal of Plant Science 2025-02-13

Blackleg, caused by Leptosphaeria maculans, is a major threat to canola production in Canada. With the exception of China, L. maculans present areas around world where cruciferous crops are grown. The pathogen can cause trade barriers international seed export due its potential risk as contaminant. most recent example China restricting seeds imported from Canada and Australia 2009. Therefore, it important assess level Blackleg infection Canadian lots dockage (seeds admixture). In this study,...

10.3390/plants5010012 article EN cc-by Plants 2016-03-01

Fusarium head blight ( FHB ) in small grain cereals is primarily caused by the members of graminearum species complex. These produce mycotoxins infected grains, deoxynivalenol DON ); acetylated derivatives , 3‐acetyl‐ (3‐ ADON and 15‐acetyl‐ (15‐ nivalenol NIV ). This study reports isolation cerealis winter wheat heads for first time Canada. A phylogenetic analysis based on TRI 101 gene F. species‐specific primers revealed two : sensu stricto (127 isolates) (five isolates). Chemotype...

10.1111/ppa.12329 article EN Plant Pathology 2014-11-17

Fusarium mycotoxins, deoxynivalenol (DON) and nivalenol (NIV) act as virulence factors are essential for symptom development after initial infection in wheat. To date, 16 genes have been identified the biosynthesis pathway. However, a comparative gene expression analysis different chemotypes of F. graminearum response to fusarium head blight (FHB) remains be explored. Therefore, this study, nine that involved trichothecene were analysed among 3-acetyldeoxynivalenol (3-ADON),...

10.3389/fmicb.2016.01229 article EN cc-by Frontiers in Microbiology 2016-08-07

Contamination of wheat grains with Fusarium mycotoxins and their modified forms is an important issue in industry. The objective this study was to analyse the deoxynivalenol (DON) deoxynivalenol-3-glucosides (D3G) content Canadian spring cultivars grown two locations, inoculated a mixture 3-acetyldeoxynivalenol (3-ADON)-producing graminearum strains 15-acetlyldeoxynivalenol (15-ADON)-producing F. strains. According analysis variance, significant differences were observed among for head...

10.1080/19440049.2016.1198050 article EN Food Additives & Contaminants Part A 2016-06-06

Positive-negative PCR assays based on the genes involved in trichothecene biosynthesis pathway are useful assessing risk of contamination grain and important epidemiological studies. A single detection method structural gene sequence TRI13 has been developed to predict 3-ADON, 15-ADON NIV chemotypes China. The chemotypic differences deletions within gene. objective this study was assess reliability using primer differentiate F. graminearum Canada. In study, we found that, cannot be used...

10.3390/ijms12096164 article EN International Journal of Molecular Sciences 2011-09-20

Wheat nurseries and fields throughout Canada were surveyed annually, from 2020 to 2023, for the presence of leaf rust, caused by Puccinia triticina Erikss. Infected leaves collected, then single pustule isolates analyzed virulence on a set 16 differential lines. In there 29 unique phenotypes found among 230 isolates, in 2021 37 153 2022, 47 246 69 384 2023. The most common over this 4-year period Manitoba Saskatchewan MNPS, TNBJ, MBDS MLPS. Ontario these TCTS, MBTN TBRD, while Quebec MBTN,...

10.1080/07060661.2024.2425954 article EN Canadian Journal of Plant Pathology 2024-12-03
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