A5056417722- Aortic aneurysm repair treatments
- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- Adipose Tissue and Metabolism
- Aortic Disease and Treatment Approaches
- Receptor Mechanisms and Signaling
- Cardiovascular Disease and Adiposity
- Infectious Aortic and Vascular Conditions
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiac, Anesthesia and Surgical Outcomes
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Apelin-related biomedical research
- Connective tissue disorders research
- Atherosclerosis and Cardiovascular Diseases
- Lipoproteins and Cardiovascular Health
- Heart Rate Variability and Autonomic Control
- Regulation of Appetite and Obesity
- Neuropeptides and Animal Physiology
- Blood Pressure and Hypertension Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Birth, Development, and Health
- Stress Responses and Cortisol
- Toxic Organic Pollutants Impact
- Cancer, Hypoxia, and Metabolism
- Pharmacological Effects and Assays
University of Kentucky
2015-2024
Nutrition Sciences (Belgium)
2021
Cedars-Sinai Medical Center
2019
Stanford University
2018
Cleveland Clinic
2018
The University of Texas Health Science Center at Houston
2018
Georgetown University Medical Center
2017
University of California, Los Angeles
2017
Georgetown University
2017
Wenzhou Medical University
2013-2016
Increased plasma concentrations of angiotension II (Ang II) have been implicated in atherogenesis. To examine this relationship directly, we infused Ang or vehicle for 1 month via osmotic minipumps into mature apoE–/– mice. These doses did not alter arterial blood pressure, body weight, serum cholesterol concentrations, distribution lipoprotein cholesterol. However, infusions promoted an increased severity aortic atherosclerotic lesions. II–induced lesions were predominantly lipid-laden...
Objective— We sought to define the temporal characteristics of angiotensin II (AngII)-induced abdominal aortic aneurysms (AAAs) and provide mechanistic insight into development this vascular pathology in apolipoprotein E-deficient (apoE-/-) mice. Methods Results— Male apoE-/- mice were infused with AngII for 1 56 days. Suprarenal arteries sequentially sectioned, cellular features defined by histologic immunocytochemical techniques. The initial identified event was medial accumulation...
Obesity is associated with a state of chronic, low-grade inflammation characterized by abnormal cytokine production and macrophage infiltration into adipose tissue, which may contribute to the development insulin resistance. During immune responses, tissue macrophages dependent on expression osteopontin, an extracellular matrix protein proinflammatory that promotes monocyte chemotaxis cell motility. In present study, we used murine model diet-induced obesity examine role osteopontin in...
Virtually every blood vessel in the body is surrounded to some degree by adipose tissue. A potential role for perivascular tissue as a neurohumoral regulator of vascular responsiveness was studied. Thoracic aortae obtained from male Sprague-Dawley rats were cut into rings use standard vitro smooth muscle bath set-up. The vessels either cleaned surrounding or left intact. Contractile responses KCl and phenylephrine well relaxation acetylcholine, isoproterenol sodium nitroprusside not...
Obesity, an inflammatory condition linked to cardiovascular disease, is associated with expansion of adipose tissue. Highly prevalent coplanar polychlorinated biphenyls (PCBs) such as 3,3',4,4'-tetrachlorobiphenyl (PCB-77) accumulate in tissue because their lipophilicity and increase obesity. However, the effects PCBs on adipocytes, obesity, obesity-associated disease are unknown.In this study we examined vitro vivo PCB-77 adipocyte differentiation, proinflammatory adipokines, morphology,...
Men and women differ in circulating lipids coronary artery disease (CAD). While sex hormones such as estrogens decrease CAD risk, hormone replacement therapy increases risk. Biological is determined by chromosomes, but effects of chromosomes on atherosclerosis are unknown. Here, we use mouse models to separate atherosclerosis, intestinal fat metabolism. We assess multiple experimental paradigms that distinguish male or female gonads. Pro-atherogenic greater XX than XY mice, indicating a...
Background: Ascending thoracic aortic aneurysm (ATAA) is caused by the progressive weakening and dilatation of wall can lead to dissection, rupture, other life-threatening complications. To improve our understanding ATAA pathogenesis, we aimed comprehensively characterize cellular composition ascending identify molecular alterations in each cell population human tissues. Methods: We performed single-cell RNA sequencing analysis tissues from 11 study participants, including 8 patients with (4...
In obesity-related hypertension, activation of the renin-angiotensin system (RAS) has been reported despite marked fluid volume expansion. Adipose tissue expresses components RAS and is markedly expanded in obesity. This study evaluated changes adipose systemic diet-induced obese hypertensive rats. was quantified compared with primary sources for circulating RAS. Male Sprague-Dawley rats were fed either a low-fat (LF; 11% kcal as fat) or moderately high-fat (32% diet 11 wk. After 8 wk,...
Objective— Angiotensin II (AngII) infusion into hyperlipidemic mice leads to the rapid formation of atherosclerotic lesions and abdominal aortic aneurysms (AAAs). To define role matrix metalloproteinases (MMPs) in development these vascular pathologies, we administered broad-spectrum MMP inhibitor doxycycline saline- AngII-infused LDL receptor−/− mice. Methods Results— Mice were placed on a high-fat diet for 1 week before with either saline or AngII (1000 ng · kg −1 min ) via osmotic pumps...
We have recently demonstrated that chronic infusion of Angiotensin II into apoE−/− mice promotes the development abdominal aortic aneurysms. To determine involvement specific receptors in this response, we co‐infused (1000 ng kg −1 min for 28 days) with losartan (30 mg day ) or PD123319 (3 to antagonize AT1 and AT2 receptors, respectively. Infusion promoted aneurysms 70% mature female mice. The formation was totally inhibited by co‐infusion ; P =0.003). In contrast, resulted a marked...
Objective— Obesity promotes macrophage infiltration into adipose tissue and is associated with increases in several cardiovascular diseases. Infusion of angiotensin II (AngII) to mice induces formation abdominal aortic aneurysms (AAAs) profound medial adventitial infiltration. We sought determine whether obesity proinflammatory cytokines periaortic surrounding aortas AngII-induced AAAs. Methods Results— Hypertrophied white adipocytes surrounded aortas, whereas brown thoracic obese mice. mRNA...
Background— Hypercholesterolemia-induced atherosclerosis is attenuated by either pharmacological antagonism of AT 1 receptors or 1A receptor deficiency. However, the mechanism underlying pronounced responses to angiotensin II (Ang II) has not been determined. We hypothesized that hypercholesterolemia stimulates production peptides provide a rationale for profound effect deficiency on atherogenesis. Methods and Results— Atherosclerotic lesions were analyzed in LDL receptor–deficient mice....
Infusion of ANG II in hyperlipidemic mice augments atherosclerosis and causes formation abdominal aortic aneurysms (AAAs). The purpose this study was to define the contribution II-induced hypertension these vascular pathologies. Male apolipoprotein E (apoE)- LDL receptor (LDLr)-deficient were infused with (1,000 ng.kg(-1).min(-1)) or norepinephrine (NE; 5.6 mg.kg(-1).day(-1)) for 28 days. NE increased mean arterial pressure (MAP; II, 133 +/- 2.8; NE, 129 13 mmHg) a similar extent compared...
The role of the renin angiotensin system (RAS) in atherosclerosis is complex because involvement multiple peptides and receptors. Renin rate-limiting enzyme production all peptides. To determine effects inhibition on atherosclerosis, we administered novel inhibitor aliskiren over a broad dose range to fat-fed LDL receptor-deficient (Ldlr(-/-)) mice. resulted striking reductions atherosclerotic lesion size both aortic arch root. Subsequent studies demonstrated that cultured macrophages...
The purpose of this study was to determine the effect chronic angiotensin II (AngII) infusion on severity atherogenic process in low density lipoprotein (LDL) receptor -/- mice with established lesions. LDL receiving a diet enriched cholesterol, saturated fat, and cholate, were infused saline or AngII (500 ng/kg/min) for 28 days. Systolic blood pressure increased following 7 days infusion, followed by decline baseline levels at days, despite continued infusion. Serum cholesterol not...
Angiotensin II (Ang II) contributes to vascular pathology in part by stimulating NADPH oxidase activity, leading increased formation of superoxide (O2-). We reported that O2- levels, and expression the p47phox subunit are human abdominal aortic aneurysms (AAAs). Here, we tested hypothesis deletion will attenuate oxidative stress AAA Ang II-infused apoE-/- mice.Male apoE-/-p47phox-/- mice received saline or (1000 ng x kg(-1) min(-1)) infusion for 28 days, after which weight maximal diameter...
Adipose tissue expresses components of the renin-angiotensin system (RAS). Angiotensin converting enzyme (ACE2), a new component RAS, catabolizes vasoconstrictor peptide ANG II to form vasodilator angiotensin 1-7 [ANG-(1-7)]. We examined whether adipocytes express ACE2 and its regulation by manipulation RAS high-fat (HF) feeding. mRNA expression increased (threefold) during differentiation 3T3-L1 was not regulated RAS. Male C57BL/6 mice were fed low- (LF) or diets for 1 wk 4 mo. At HF...
The presence of angiotensinogen messenger RNA (mRNA) was detected in rat vascular and adipose tissue. Angiotensinogen mRNA aorta localized the adventitia surrounding tissue, not smooth muscle. Freshly dispersed cultured endothelial aortic muscle cells did contain detectable amounts mRNA. In addition to periaortic present other fat depots both brown white types. To examine regulation gene expression, Sprague-Dawley rats were treated with angiotensin converting enzyme inhibitor or underwent...