A5101914772- Carcinogens and Genotoxicity Assessment
- Chemical Reactions and Isotopes
- Genetic factors in colorectal cancer
- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Pharmacogenetics and Drug Metabolism
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Biopolymer Synthesis and Applications
- Multiple and Secondary Primary Cancers
- Statistical Methods in Clinical Trials
- Cancer Genomics and Diagnostics
- BRCA gene mutations in cancer
- Food Quality and Safety Studies
- Amino Acid Enzymes and Metabolism
- Molecular Biology Techniques and Applications
- Radiation Effects and Dosimetry
- DNA Repair Mechanisms
- Biomarkers in Disease Mechanisms
- Advanced Glycation End Products research
- Free Radicals and Antioxidants
- Genomic variations and chromosomal abnormalities
- Connexins and lens biology
- Biosimilars and Bioanalytical Methods
Hospital Pulido Valente
1999
Universidade Nova de Lisboa
1989-1999
University of Lisbon
1989-1999
Nova Medical (United States)
1996
Previous studies have suggested that free radicals and related species play a role in lens damage. The molecules involved may include proteins, lipids DNA8. Focal cortical changes liquefaction been reported patients with Down's syndrome over the age of 15 years9.There is evidence supporting hypothesis trisomy 21 an increase radical reactions lipoperoxidation susceptibility. This could be due to H2O2 generation catalysed by CuZn SOD although activity other gene products coded for on...
Cytochrome b5 (b5) is increasingly recognized to be of importance for specific cytochrome P450 (CYP) activities. We developed human b5/CYP-competent mutagenicity tester bacteria study the role in bioactivation activity CYP. These new were derived from previously engineered CYP-competent Escherichia coli K12 strain MTC, containing a bi-plasmid system co-expression CYP form (CYP1A2, 2A6 or 2E1) with b5, and NADPH reductase (RED), resulting BTC-b5-1A2, BTC-b5-2A6 BTC-b5-2E1, respectively. The...
The flavonol kaempferol is widely found in the diet and directly mutagenic some short-term tests, such as induction of chromosomal aberrations cukaryotic cells. presence exogenous metabolizing systems enhances its mutagenicity. We have evaluated role cytochromes P450 by V79 results obtained suggest that there a time-dependent biotransformation to quercetin, P450, assessed high pressure liquid chromatography. Quercetin seems contribute mutagenicity microsomal systems. On other hand, direct...
Journal Article Structural requirements for mutagenicity of flavonoids upon nitrosation. A structure—activity study Get access José Rueff, Rueff 3 1Department Genetics, Faculty Medical Sciences UNL, R. da Junqueira 96, P-1300 Lisbon 3To whom correspondence should be addressed Search other works by this author on: Oxford Academic PubMed Google Scholar Jorge Gaspar, Gaspar António Laires Lisbon2Faculty and Technology UNLP-2825 Monte Caparica Portugal Mutagenesis, Volume 10, Issue 4, July 1995,...
Epidemiological studies have led to the suggestion that a genetic basis may exist in individual variation predisposition cancer. Interindividual differences human toxicological response carcinogenic exposure been attributed heritable polymorphisms metabolism, namely glutathione S-transferases (GSTs) coding for enzymes are known be detoxifiers of carcinogens. Within GST mu class, there is specific isozyme frequently lacking. To check whether or not this association exists Portuguese...
The glutathione S-transferases appear to form part of a protective mechanism against the development cancer where environmental chemical carcinogens are involved. In humans one member mu class gene family (GSTM1) has been shown be polymorphic and is only expressed in ~50% individuals. Previous studies have possible link between null phenotype susceptibility but equivocal regarding stomach cancer. To evaluate any association Portuguese gastric individuals with GSTM1 variability, we performed...
AIMS: p53 gene mutations are the most common genetic changes known to occur in human cancer. In previous studies, presence of alterations has been linked null phenotype glutathione S-transferase mu (GSTM1). GSTM1 appears be part a protective mechanism against development cancers which environmental chemical carcinogens involved. To screen for such an association stomach cancer, allelic loss and genomic instability genotypes were investigated gastric tumour DNA samples from 113 patients....
The mutagenic activity of glycine upon nitrosation was studied in the Ames tester strains TA98, TA100, TA102, and TA104. results obtained show that at acidic pH values presence Cl− can react with nitrite giving rise to genotoxic compounds used. When these experiments were carried out gastric juice genotoxicity observed associated concentration different samples. nature mechanism genetic lesion induced by ultimate genotoxicant arising from are not fully understood. Primary amines (e.g., amino...
Genetic epidemiological studies are useful for the knowledge of association markers and genes involved in diseases. In present work, we studied frequency four adenomatous polyposis coli intragenic RFLP often used risk evaluation a population 10 familial patients from unrelated Portuguese families not sharing same mutation, 55 healthy volunteers. We compared obtained to normal affected populations results already reported other populations. observed allelic frequencies that agree with...
Owing to the large size of APC gene, responsible for familial adenomatous polyposis, direct screening individual mutations is not a practical approach. In present study we establish methodology fluorescence based semi-automated DNA analysis perform presymptomatic diagnosis members at risk from 11 Portuguese FAP families with three (CA)n markers flanking MBC, CB26, and YN5.64, four intragenic RFLPs. Haplotypes were constructed on basis genotypes their segregation through generations followed....
The flavonol kaempferol is widely found in the diet and directly mutagenic some short-term tests, such as induction of chromosomal aberrations cukaryotic cells. presence exogenous metabolizing systems enhances its mutagenicity. We have evaluated role cytochromes P450 by V79 results obtained suggest that there a time-dependent biotransformation to quercetin, P450, assessed high pressure liquid chromatography. Quercetin seems contribute mutagenicity microsomal systems. On other hand, direct...
Martins, G; Alves, M; Dias, J; Santos, R; Neves, B C; Mafra, A P; Ramos, S; Mexia, Quina, Rueff, Monteiro, C Author Information
The mutagenic activity of glycine upon nitrosation was studied in the Ames tester strains TA98, TA100, TA102, and TA104. results obtained show that at acidic pH values presence Cl− can react with nitrite giving rise to genotoxic compounds used. When these experiments were carried out gastric juice genotoxicity observed associated concentration different samples. nature mechanism genetic lesion induced by ultimate genotoxicant arising from are not fully understood. Primary amines (e.g., amino...