A5052088083- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Silkworms and Sericulture Research
- Insect behavior and control techniques
- Lepidoptera: Biology and Taxonomy
- CRISPR and Genetic Engineering
- Plant and animal studies
- Insect Utilization and Effects
- RNA and protein synthesis mechanisms
- Nematode management and characterization studies
- Phytoplasmas and Hemiptera pathogens
- Plant Reproductive Biology
- Animal Behavior and Reproduction
- RNA Interference and Gene Delivery
- Insect and Arachnid Ecology and Behavior
- Genomics, phytochemicals, and oxidative stress
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Trypanosoma species research and implications
- Transgenic Plants and Applications
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Animal Genetics and Reproduction
University of California, Irvine
2016-2023
Early lineage diversification is central to understand what mutational events drive species divergence. Particularly, gene misregulation in interspecific hybrids can inform about genes and pathways underlie hybrid dysfunction. In Drosophila hybrids, how regulatory evolution impacts different reproductive tissues remains understudied. Here, we generate a new genome assembly annotation willistoni analyse the patterns of transcriptome divergence between two allopatrically evolved D. subspecies,...
Abstract A detailed knowledge of gene function in the monarch butterfly is still lacking. Here we generate a genome assembly from Mexican nonmigratory population and used RNA-seq data 14 biological samples for annotation to construct an atlas portraying breadth expression during most life cycle. Two thirds genes show changes, with long noncoding RNAs being particularly finely regulated adulthood, male-biased four times more common than female-biased. The two portions heterochromosome Z , one...
Gene clusters of recently duplicated genes are hotbeds for evolutionary change. However, our understanding how mutational mechanisms and forces shape the structural functional evolution these is hindered by high sequence identity among copies, which typically results in their inaccurate representation genome assemblies. The presumed testis-specific, chimeric gene Sdic originated, tandemly expanded Drosophila melanogaster, contributing to increased male-male competition. Using various types...
Abstract Gene families underlie genetic innovation and phenotypic diversification. However, our understanding of the early genomic functional evolution tandemly arranged gene remains incomplete as paralog sequence similarity hinders their accurate characterization. The Drosophila melanogaster-specific family Sdic is repeated impacts sperm competition. We scrutinized in 20 geographically diverse populations using reference-quality genome assemblies, read-depth methodologies, qPCR, finding...
Speciation can occur through the presence of reproductive isolation barriers that impede mating, restrict cross-fertilization, or render inviable/sterile hybrid progeny. The D. willistoni subgroup is ideally suited for studies speciation, with examples both allopatry and sympatry, a range barriers, availability one species complete genome sequence to facilitate genetic divergence. w. has largest geographic distribution among members Drosophila subgroup, spanning from Argentina southern...
Sperm competition is a postcopulatory sexual selection mechanism in species which females mate with multiple males. Despite its evolutionary relevance shaping male traits, the genetic mechanisms underlying sperm are poorly understood. A recently originated multigene family specific to Drosophila melanogaster, Sdic, important for outcome of doubly mated females, although mechanistic nature this phenotype remained unresolved. Here, we compared second either Sdic knockout or nonknockout males,...
How recently originated gene copies become stable genomic components remains uncertain as high sequence similarity of young duplicates precludes their functional characterization. The tandem multigene family Sdic is specific to Drosophila melanogaster and has been annotated across multiple reference-quality genome assemblies. Here we show the existence a positive correlation between copy number total expression, plus vast intrastrain differences in mRNA abundance among paralogs, using...
The magnitude and functional patterns of intraspecific transcriptional variation in the anophelines, including those sex-biased genes underlying sex-specific traits relevant for malaria transmission, remain understudied. As a result, how changes expression levels drive adaptation these species is poorly understood. We sequenced female, male, larval transcriptomes three populations Anopheles arabiensis from Burkina Faso. One-third were differentially expressed between populations, often...
ABSTRACT The monarch butterfly epitomizes insect biodiversity decline. Understanding the genetic basis of adaptation to a changing environment requires genomic and transcriptomic resources that better reflect its diversity while being informative about gene functionality during life cycle. We report reference-quality genome assembly from an individual resident at nonmigratory colony in Mexico, new annotation expression atlas for 14,865 genes, including 492 unreported long noncoding RNA...