A5040229528- Hemoglobin structure and function
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Porphyrin Metabolism and Disorders
- Genomics and Phylogenetic Studies
- Metabolism and Genetic Disorders
- Anaerobic Digestion and Biogas Production
- Electrochemical sensors and biosensors
- Enzyme Catalysis and Immobilization
- Microbial Community Ecology and Physiology
- Biochemical and Molecular Research
- Bacterial Genetics and Biotechnology
- 3D Printing in Biomedical Research
- Microbial Fuel Cells and Bioremediation
- RNA and protein synthesis mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Light effects on plants
- Enzyme Structure and Function
- Single-cell and spatial transcriptomics
- Photoreceptor and optogenetics research
- Protein Structure and Dynamics
- Polyamine Metabolism and Applications
Royal Netherlands Institute for Sea Research
2022-2024
University of Kaiserslautern
2018-2020
Ruhr University Bochum
2019-2020
Technische Universität Braunschweig
2011
Bacterial membranes are composed of fatty acids (FAs) ester-linked to glycerol-3-phosphate, while archaea have made isoprenoid chains ether-linked glycerol-1-phosphate. Many archaeal species organize their membrane as a monolayer membrane-spanning lipids (MSLs). Exceptions this “lipid divide” the production by some bacterial (ether-bound) MSLs, formed tail-to-tail condensation FAs resulting in formation (iso) diabolic (DAs), which likely precursors paleoclimatological relevant branched...
Bacillus subtilis forms acetoin under anaerobic fermentative growth conditions and as a product of the aerobic carbon overflow metabolism. Acetoin formation from pyruvate requires α-acetolactate synthase acetolactate decarboxylase, both encoded by alsSD operon. The alsR gene, encoding LysR-type transcriptional regulator AlsR, was found to be essential for in vivo expression response acetate accumulation, addition acetate, low pH, stationary phase. expressions operon regulatory gene were...
Recombinant production of heme proteins in Escherichia coli is often limited by the availability host. Therefore, several methods, including reconstitution after but prior to purification or HPEX system, conferring ability take up external have been developed and used past. Here we describe use apathogenic E. strain Nissle 1917 (EcN) as a suitable host for recombinant proteins. EcN has an advantage over commonly lab strains that it able from environment through receptor ChuA. Expression...
Abstract Background Heme proteins and heme-derived molecules are essential in numerous cellular processes. Research into their vitro functionality requires the production of large amounts protein. Unfortunately, high yield expression is hampered by lack E. coli strains naturally capable taking up heme from medium. We recently reported use probiotic strain Nissle 1917 (EcN) to sufficiently produce containing proteins, as it encodes outer membrane receptor, ChuA, which allows for natural...
Summary Organisms have evolved signal transduction systems to quickly adapt their lifestyle internal and environmental changes. While protein kinases two‐component are widely distributed in Bacteria , they also found Archaea but less diversified abundant. In this work, we analysed the function of kinase RdmS its role a putative system methanogenic archaeon Methanosarcina acetivorans . is encoded upstream regulator MsrF, which activates expression corrinoid/methyltransferase fusion MtsD....
Abstract Background: Heme proteins and heme-derived molecules are essential in numerous cellular processes. Research into their vitro functionality requires the production of large amounts protein. Unfortunately, high yield expression is hampered by lack E. coli strains naturally capable taking up heme from medium. We recently reported use probiotic strain Nissle 1917 (EcN) to sufficiently produce containing proteins, as it encodes outer membrane receptor, ChuA, which allows for natural...
The sensor kinases MsmS and RdmS from the methanogenic archaeon Methanosarcina acetivorans are multidomain proteins containing a covalently linked heme cofactor. This cofactor is connected via single cysteine residue in GAF domain. Although both were shown to display redox-dependent control of downstream kinase module, this property appears be independent We therefore envision an additional role for In order learn more about binding pocket its constitution, UV-vis spectroscopy combination...
Abstract Background: Heme proteins and heme-derived molecules are essential in numerous cellular processes. Research into their vitro functionality requires the production of large amounts protein. Unfortunately, high yield expression is hampered by lack E. coli strains naturally capable taking up heme from medium. We recently reported use probiotic strain Nissle 1917 (EcN) to sufficiently produce containing proteins, as it encodes outer membrane receptor, ChuA, which allows for natural...
Bacterial membranes are composed of fatty acids (FAs) ester-linked to glycerol-3-phosphate, while archaea possess made isoprenoid chains ether-linked glycerol-1-phosphate. Many archaeal species organize their membrane as a monolayer membrane-spanning lipids (MSLs). Exceptions this ‘lipid divide’ the production by some bacterial (ether-bound) MSLs, formed tail-tail condensation resulting in formation ( iso ) diabolic (DAs), which likely precursors paleoclimatological relevant branched...