A5035312412- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Renal and related cancers
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetics, Bioinformatics, and Biomedical Research
- Protein Degradation and Inhibitors
- Animal Genetics and Reproduction
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- 3D Printing in Biomedical Research
University of Utah
2014-2025
Huntsman Cancer Institute
2014-2025
Howard Hughes Medical Institute
2014-2025
Gwangju Institute of Science and Technology
2007-2010
Postnatal spermatogonial stem cells (SSCs) progress through proliferative and developmental stages to populate the testicular niche prior productive spermatogenesis. To better understand, we conducted extensive genomic profiling at multiple postnatal on subpopulations enriched for particular markers (THY1, KIT, OCT4, ID4, or GFRa1). Overall, our profiles suggest three broad populations of spermatogonia in juveniles: (1) epithelial-like (THY1 + ; high GFRa1), (2) more abundant...
Abstract Spermatogonial stem cells balance self-renewal with differentiation and spermatogenesis to ensure continuous sperm production. Here, we identify roles for the transcription factor zinc finger BTB domain-containing protein 16 (ZBTB16; also known as promyelocytic leukemia (PLZF)) in juvenile mouse undifferentiated spermatogonia (uSPG) promoting cell-cycle progression maintain uSPG transit-amplifying states. Notably, ZBTB16, Spalt-like 4 (SALL4) SRY-box 3 (SOX3) colocalize at over...
The primary regulator of spermatogenesis, a highly ordered and tightly regulated developmental process, is an intrinsic genetic program involving male germ cell-specific genes. We analyzed the mouse spermatocyte UniGene library containing 2155 gene-oriented transcript clusters. predict that 11% these genes are testis-specific systematically identified 24 authentic specifically abundantly expressed in testis via silico vitro approaches. Northern blot analysis disclosed various...
The transcription factor Oct4/Pou5f1 is a component of the regulatory circuitry governing pluripotency and widely used to induce from somatic cells. Here we domain swapping mutagenesis study Oct4's reprogramming ability, identifying redox-sensitive DNA binding domain, cysteine residue (Cys48), as key determinant differentiation. Oct4 Cys48 sensitizes protein oxidative inhibition activity promotes oxidation-mediated ubiquitylation. Pou5f1 C48S point mutation has little effect on...
Abstract The transcription factor Oct4/Pou5f1 is a component of the regulatory circuitry governing pluripotency and widely used to induce from somatic cells. Here we use domain swapping mutagenesis study Oct4’s reprogramming ability, identifying redox-sensitive DNA binding cysteine residue (Cys48) as key determinant differentiation. Oct4 Cys48 sensitizes protein oxidative inhibition activity promotes oxidation-mediated ubiquitylation. Pou5f1 C48S point mutation has little effect on...
You have accessJournal of UrologyInfertility: Basic Research & Pathophysiology II1 Apr 2017PD08-03 PROFILING OF HUMAN ADULT SPERMATOGONIAL STEM CELLS REVEALS TRANSCRIPTION AND SIGNALING PROGRAMS FOR SELF RENEWAL DIFFERENTIATION Jingtao Guo, Edward J. Grow, Chongil Yi, Douglas T. Carrell, James M. Hotaling, and Bradley R. Cairns GuoJingtao Guo More articles by this author , GrowEdward Grow YiChongil Yi CarrellDouglas Carrell HotalingJames Hotaling CairnsBradley View All Author...