A5084354052- Sinusitis and nasal conditions
- Angiogenesis and VEGF in Cancer
- Head and Neck Surgical Oncology
- Cancer, Hypoxia, and Metabolism
- Allergic Rhinitis and Sensitization
- DNA Repair Mechanisms
- Olfactory and Sensory Function Studies
- DNA and Nucleic Acid Chemistry
- Asthma and respiratory diseases
- Nasal Surgery and Airway Studies
- Ion Channels and Receptors
- Oral and Maxillofacial Pathology
- Cancer, Lipids, and Metabolism
- Polyomavirus and related diseases
- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Advanced biosensing and bioanalysis techniques
- Hearing, Cochlea, Tinnitus, Genetics
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Facial Trauma and Fracture Management
- Head and Neck Cancer Studies
- Vestibular and auditory disorders
- Drug Transport and Resistance Mechanisms
Kagawa University
2015-2025
Hiroshima University
2023
Hokkaido University
2010-2022
Tokai University Hachioji Hospital
2022
Tokai University
2022
Showa University Fujigaoka Hospital
2016-2021
Nagoya Medical Center
2020-2021
Onomichi General Hospital
2021
Saitama Children's Medical Center
2013-2016
Mito Saiseikai General Hospital
2012-2014
Abstract Tumour blood vessels are gateways for distant metastasis. Recent studies have revealed that tumour endothelial cells (TECs) demonstrate distinct phenotypes from their normal counterparts. We demonstrated features of TECs different depending on malignancy, suggesting communicate with surrounding cells. However, the contribution to metastasis has not been elucidated. Here, we show actively promote through a bidirectional interaction between and TECs. Co-implantation isolated highly...
Tumour stromal cells differ from its normal counterpart. We have shown that tumour endothelial (TECs) isolated tissues are also abnormal. Furthermore, we found mRNAs of vascular growth factor-A (VEGF-A) and cyclooxygenase-2 (COX-2) were upregulated in TECs. Vascular COX-2 angiogenic factors their contain an AU-rich element (ARE). element-containing reportedly stabilised by Hu antigen R (HuR), which is exported to the cytoplasm. Normal cell (NEC) two types TECs isolated. evaluated correlation...
Background Increasing evidence indicates that tumor endothelial cells (TEC) differ from normal (NEC). Our previous reports also showed TEC were different NEC. For example, have chromosomal abnormality and proangiogenic properties such as high motility proliferative activity. However, the mechanism by which acquire a specific character remains unclear. To investigate this mechanism, we focused on tumor-derived microvesicles (TMV). Recent studies shown TMV contain numerous types of bioactive...
There is much evidence that hypoxia in the tumor microenvironment enhances progression. In an earlier study, we reported abnormal phenotypes of tumor-associated endothelial cells such as those resistant to chemotherapy and chromosomal instability. Here investigated role acquisition abnormalities cells. Tumor-associated isolated from human xenografts showed abnormalities, >30% which were aneuploidy. Aneuploidy was also shown by simultaneous in-situ hybridization for chromosome 17...
Molecules that are highly expressed in tumour endothelial cells (TECs) may be candidates for specifically targeting TECs. Using DNA microarray analysis, we found the lysyl oxidase (LOX) gene was upregulated TECs compared with its expression normal (NECs). LOX is an enzyme enhances invasion and metastasis of cells. However, there no reports on function isolated NECs were to investigate inhibition vivo growth also assessed using β-aminopropionitrile (BAPN). higher than NECs. knockdown...
We reported that tumor endothelial cells (TECs) differ from normal (NECs) in many aspects, such as gene expression profiles. Although CXCR7 is reportedly highly expressed blood vessels of several tumors, its function TECs still unknown. To investigate this role, we isolated mouse A375SM xenografts, and compared them with NECs dermis. After confirming upregulation TECs, analyzed using siRNA inhibitor; CCX771. CCX771 inhibited migration, tube formation resistance to serum starvation but not...
We purified a mouse DNA repair enzyme having apurinic/apyrimidinic endonuclease, 3'-phosphatase, 3'-5'-exonuclease and 3' diesterase activities, designated the as APEX nuclease. A cDNA clone for was isolated from spleen library using probes of degenerate oligonucleotides deduced N-terminal amino acid sequence enzyme. The complete nucleotide (1.3 kilobases) determined. Northern hybridization this showed that size its mRNA is about 1.5 kilobases. predicted (APEX nuclease cDNA) indicates...
Tumor angiogenesis is necessary for progression and metastasis of solid tumor. blood vessels are morphologically different from their normal counterparts. In this study, we isolated tumor endothelial cells (TECs) revealed abnormalities. We have compared the gene expression profiles TECs (NECs) by microarray analysis found that several genes were upregulated in TECs. Expression chemokine receptor CXCR7 mRNA was higher than NECs. However, information regarding renal cell carcinoma limited. its...
Tumor blood vessels play an important role in tumor progression and metastasis. It has been reported that endothelial cells (TECs) exhibit highly angiogenic phenotypes compared with those of normal (NECs). TECs show higher proliferative migratory abilities than NECs, together upregulation vascular growth factor (VEGF) VEGF receptor 2 (VEGFR2). Furthermore, stem cell markers such as Sca-1, CD90, multidrug resistance 1 are upregulated TECs, suggesting stem-like exist vessels. In this study, to...
Multi-drug resistance (MDR) of cancers to chemotherapy including doxorubicin (DOX) is mediated by several factors. To design an effective therapy for the treatment chemotherapy-resistant cancers, it essential explore elements responsible mediating MDR. However, exploring these factors in detail a wide range tumor types challenging as critical analytical steps are involved. Here, we demonstrated way MDR without performing analysis at molecular level cells. The sensitivities 15 different...
Tumor blood vessels play an important role in tumor progression and metastasis. We previously reported that endothelial cells ( TEC ) exhibit several altered phenotypes compared with normal NEC ). For example, have chromosomal abnormalities are resistant to anticancer drugs. Furthermore, contain stem cell‐like populations high aldehyde dehydrogenase ALDH activity proangiogenic properties low . However, the association between drug resistance remains unclear. In present study, we found mRNA...
// Takayuki Hojo 1, 2, * , Nako Maishi Alam Mohammad Towfik 3 Kosuke Akiyama 1 Noritaka Ohga 4 Masanobu Shindoh 5 Yasuhiro Hida 6 Kazuyuki Minowa Toshiaki Fujisawa 2 and Kyoko Vascular Biology, Frontier Research Unit, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan Department of Dental Anesthesiology, University Graduate School 060-8586, Radiology, Oral Diagnosis Pathology Cardiovascular Thoracic Surgery, 060-8638, These authors contributed equally to this work...
Abstract Tumor endothelial cells (TECs) reportedly exhibit altered phenotypes. We have demonstrated that TECs acquire drug resistance with the upregulation of P-glycoprotein (P-gp, ABCB1), contrary to traditional assumptions. Furthermore, P-gp expression was higher in highly metastatic tumors than those low tumors. However, detailed mechanism differential remains unclear. miRNA identified tumor extracellular vesicles (EVs) and roles cell were analyzed vitro vivo. In present study, we found...
Recent studies have reported that stromal cells contribute to tumor progression. We previously demonstrated endothelial (TEC) characteristics were different from those of normal (NEC). Furthermore, we performed gene profile analysis in TEC and NEC, revealing suprabasin (SBSN) was upregulated compared with NEC. However, its role is still unknown. Here showed SBSN expression higher isolated human mouse than knockdown inhibited the migration tube formation ability TEC. also AKT pathway a...