A5008458073- Ubiquitin and proteasome pathways
- interferon and immune responses
- NF-κB Signaling Pathways
- PARP inhibition in cancer therapy
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- RNA and protein synthesis mechanisms
- Calcium signaling and nucleotide metabolism
- Advanced Biosensing Techniques and Applications
- Autophagy in Disease and Therapy
- Neuropeptides and Animal Physiology
- Diabetes Treatment and Management
- Protein Degradation and Inhibitors
- Genetics and Neurodevelopmental Disorders
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
- Toxin Mechanisms and Immunotoxins
- CRISPR and Genetic Engineering
Radboud Institute for Molecular Life Sciences
2021-2025
Radboud University Medical Center
2021-2025
Radboud University Nijmegen
2021-2025
Oncode Institute
2021-2024
Max Planck Institute of Molecular Physiology
2024
Goethe University Frankfurt
2015-2024
Abstract Signalling through TNFR1 modulates proinflammatory gene transcription and programmed cell death, its impairment causes autoimmune diseases cancer. NEDD4-binding protein 1 (N4BP1) is a critical suppressor of cytokine production that acts as regulator innate immune signalling inflammation. However, our current understanding about the molecular properties enable N4BP1 to exert suppressive potential remain limited. Here, we show novel linear ubiquitin reader negatively regulates NFκB by...
Abstract Transcription-blocking DNA lesions are specifically targeted by transcription-coupled nucleotide excision repair (TC-NER), which removes a broad spectrum of to preserve transcriptional output and thereby cellular homeostasis counteract aging. TC-NER is initiated the stalling RNA polymerase II at lesions, triggers assembly TC-NER-specific proteins CSA, CSB UVSSA. WD40-repeat containing protein, substrate receptor subunit cullin-RING ubiquitin ligase complex composed DDB1, CUL4A/B...
Olaparib is a widely used PARP inhibitor for the treatment of BRCA-mutated cancers. To comprehensively understand drug's clinical impact, measuring its interactions with intended on- and off-targets crucial. In this study, olaparib's were profiled using photoaffinity labeling, powerful, proteome-wide method studying direct between drug protein targets. A novel probe was designed in proteomic screening to discover targets olaparib human proteome. The probe, incorporating pre-installed biotin...
Rpn13 is an intrinsic ubiquitin receptor of the 26S proteasome regulatory subunit that facilitates substrate capture prior to degradation. Here we show C-terminal region binds tetratricopeptide repeat (TPR) domain SGTA, a cytosolic factor implicated in quality control mislocalised membrane proteins (MLPs). The overexpression SGTA results substantial increase steady-state MLP levels, consistent with effect on proteasomal However, this strongly dependent upon interaction component Rpn13....
Transcription-blocking lesions are specifically targeted by transcription-coupled nucleotide excision repair (TC-NER), which prevents DNA damage-induced cellular toxicity and maintains proper transcriptional processes. TC-NER is initiated the stalling of RNA polymerase II (RNAPII), triggers assembly TC-NER-specific proteins, namely CSB, CSA UVSSA, collectively control drive progression. Previous research has revealed molecular functions for these however, exact mechanisms governing...
Transcription-blocking DNA lesions are specifically targeted by transcription-coupled nucleotide excision repair (TC-NER), which removes a broad spectrum of to preserve transcriptional output and thereby cellular homeostasis counteract aging. TC-NER is initiated the stalling RNA polymerase II at lesions, triggers assembly TC-NER-specific proteins CSA, CSB UVSSA. WD40-repeat containing protein, substrate receptor subunit cullin-RING ubiquitin ligase complex composed DDB1, CUL4A/B RBX1 (CRL4
Abstract Signalling through TNFR1 modulates proinflammatory gene transcription and programmed cell death, its impairment causes autoimmune diseases cancer. NEDD4-binding protein 1 (N4BP1) was recently identified as a critical suppressor of cytokine production , whose mode action remained unknown. Here, we show that N4BP1 is novel linear ubiquitin reader negatively regulates NFκB signalling by unique dimerizationdependent ubiquitin-binding module named LUBIN. Dimeric strategically positions...