In the present study, antifungal activity of wood treated with Pinus halepensis branch n-hexane oily liquid extract (OLE) and Schinus terebinthifolius essential oil (EO) was evaluated against growth four phytopathogenic fungi—Bipolaris oryzae, Fusarium oxysporum, solani, Rhizoctonia solani. Air-dried samples roxburghii were autoclaved, each received 100 µL concentrated oils from P. S. terebinthifolius. The main compounds identified in EO terpinen-4-ol (18.25%), cis-β-terpineol (15.60%),...

Leaves essential oils of Syzygium cumini L. Cupressus sempervirens and Lantana camara L., from Alexandria, Egypt were analysed by Gas chromatography-mass spectrometry (GC-MS). The antibacterial antioxidant activities assessed for each oil using minimum inhibitory concentrations (MICs) 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods; respectively. main constitutes alpha-pinene (17.53%), alpha-terpineol (16.67%) alloocimene (13.55%) in S. cumini, trans-caryophyllene (15.57%) alpha-humulene...

The crude methanolic extracts from leaves of Eucalyptus camaldulensis L., E. var obtusa and gomphocephala grown in Egypt were investigated to explore their chemical composition as well antibacterial, antifungal antioxidant activities. Major phenolics found ellagic acid, quercetin 3-O-rhamnoside, 3-O-b-D-glucuronide, caffeic acid chlorogenic acid. activities examined by the 2,2′-diphenypicrylhydrazyl (DPPH) β-Carotene-linoleic assays. showed highest phenolic content, antimicrobial compared...

Aqueous, acetone, and ethanol extracts of Coccoloba uvifera L. (Polygonaceae) leaves were assessed for their antibacterial antifungal activities. The fungal pathogens Fusarium culmorum, Rhizoctonia solani, Botrytis cinerea isolated from strawberry plants, they molecularly identified through internal transcribed spacers (ITS) sequence analysis. Wood treated with extract at 3% showed the highest inhibition R. B. cinerea, F. culmorum growth, mycelial growth inhibited by 64.4%, 100%, 38.5%,...

AbstractThe history of local Egyptian mint dates back to at least 1,000 BC, when the ancient Egyptians used it as part their herbal medicine found in Ebers papyrus. Our study focused on essential oil composition and recently introduced Mentha sp. either suitability or toxicity for humans traditional medicines. Leaf oils four different sp.; spicata L. "mint", longifolia "wild mint", suaveolens "apple mint" piperita "chocolate" Egypt were analyzed using GC GC-MS. The antibacterial (agar disc...

The chemical composition of the essential oil from leaves Eucalyptus camaldulensis, camaldulensis var. obtusa and gomphocephala grown in northern Egypt was analysed by using GC-FID GC-MS techniques. antibacterial (agar disc diffusion minimum inhibitory concentration methods) antioxidant activities (2,2'-diphenypicrylhydrazyl) were examined. main oils constituents 1,8-cineole (21.75%), β-pinene (20.51%) methyleugenol (6.10%) E. camaldulensis; spathulenol (37.46%), p-cymene (17.20%) crypton...

Phenolic acids and natural extracts, as ecofriendly environmental agents, can be used bio bactericides against the growth of plant pathogenic bacteria. In this study, isolation trails from infected potato tubers stems that showed soft rot symptoms in fields revealed two bacterial isolates were initially identified through morphological, physiological, pathogenicity tests. The molecular characterization these via PCR, based on 16S rRNA region, was carried out by an analysis DNA sequence BLAST...

Background: early blight disease caused by Alternaria solani is one of the most destructive diseases tomato, reducing tomato production globally. Methods: four fungal isolates were collected from cultivars and identified through morphological characterization polymerase chain reaction (PCR) amplification internal transcript spacer (ITS) region. Plectranthus neochilus Parthenocissus quinquefolia methanol extracts bioagents Trichoderma viride Pseudomonas fluorescens used as antifungal agents...

Potent antibacterial activities of solvent extracts (methanol:n-hexane) from the branch, leaf, and root-wood Salvadora persica were examined against potato phytopathogenic bacteria, namely Pectobacterium carotovorum subsp. carotovorum, Dickeya solani, Ralostonia solanacerum, Enterobacter cloacae, Bacillus pumilus. The main chemical constituents analyzed by gas chromatography–mass spectrometry (GC/MS) in branch N-benzylbenzamide (71.08%), decane (3.17%), stigmasterol...

Bacterial metal reducers were isolated from water samples collected harsh condition locations in Egypt. Four selected isolates identified as Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculata for Copper (Cu), Iron (Fe), Cobalt (Co) Zinc (Zn) Nanoparticles (NPs) production sequentially. Nitrate reductase enzyme was assayed bacterial which demonstrated that M. hydrocarbonoclasticus have the maximum production. The produced NPs characterized by...

A C T Eleven Erwinia amylovora isolates, the causal agent of fire blight disease, were isolated from apple orchards adjacent to those pears showing disease symptoms.Initial characterization concerning morphological and biochemical traits revealed that all isolates typical E. amylovora.Furthermore, bacterial showed symptoms upon infection unripe pear fruits.Molecular these via PCR utilizing specific primers based on a region plasmid pEA29 chromosomal DNA ams-region was performed.PCR products...

The seed-borne bacterial diseases of family solanaceae cause significant economic losses worldwide. In the present study, fourteen isolates were recovered from seeds different varieties tomato, eggplant, black nightshade and tobacco. seed samples collected locations in Egypt. Isolated bacteria identified based on morphological, physiological biochemical tests as well colonial morphology aspects differential medium, yeast extract dextrose calcium carbonate (YDC) besides selective media (PA...

Molecular and Biological Identification of Erwinia amylovora Egyptian Isolates Compared with Other German Strains Thirty one isolates the enterobacterium causal agent fire blight disease were collected from different locations in Egypt Germany. The E. (Ea) identified by biochemical tests obtained results confirmed polymerase chain reaction (PCR) using 16S universal primers. showed that, all examined are percentage similarity. phylogenetic tree constructed based on DNA nucleotide sequences...

A C T Brown rot disease of potato caused by the bacterium Ralstonia solanacearum (smith) is a destructive limiting production in several areas world.Nineteen isolates were recovered from infected tubers, showing typical symptoms that had been collected Alexandria, Behera, Ismailia and Menofia Governorates.Isolates identified as R. biovar 2 (race 3), based on nutritional biochemical enzymatic features well as, standard determination tests.All at molecular level through Polymerase Chain...

Abstract The nanoparticles (NPs) formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus , and P. geniculate were tested against soft rot/blackleg genera. effects of NPs recorded on bacterial DNA, proteins, carbohydrates concentration Pectobacterium carotovorum subsp. carotovorum, Enterobacter cloacae (soft rot), Dickeya solani rot/blackleg). Treated cells showed degradation in isolated decreased proteins compared with untreated cells. Using Scanning...

The soft rot bacteria Pectobacterium and Dickeya species cause economically important diseases on potato crops.Specific efficient detection methods are essential to investigate the ecology pathogenesis of as well in seed certification programmes.Polyclonal antibodies against bacterium; carotovorum subsp.carotovorum were generated female New Zealand white rabbits.Determination optimum period collect antiserum (As) using indirect Enzyme-Linked Immunosorbent Assay (ELISA) showed that, first...

The antibacterial activity of methanolic crude extract and its subsequent fractionation into ethyl acetate, n -butanol, chloroform aqueous fractions from wood, bark leaves Ficus benghalensis L., retusa Dalbergia sissoo Roxb., Syzygium cumini (L.) Skeels, Populus nigra L. Cupressus sempervirens grown in Egypt was investigated against some pathogenic bacteria. susceptibility test evaluated by disc-diffusion minimum inhibitory concentrations methods. MCE acetate fraction were found to possess...