A5010686334- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- Advanced biosensing and bioanalysis techniques
- Peroxisome Proliferator-Activated Receptors
- Protein Degradation and Inhibitors
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cytomegalovirus and herpesvirus research
Howard Hughes Medical Institute
2020
Massachusetts Institute of Technology
2020
Whitehead Institute for Biomedical Research
2020
Broad Institute
2020
University of California, Berkeley
2016-2018
Innovative Genomics Institute
2016-2018
National Institutes of Health
2015
National Cancer Institute
2015
Abstract The Cas9 endonuclease can be targeted to genomic sequences by programming the sequence of an associated single guide RNA (sgRNA). For unknown reasons, activity these Cas9–sgRNA combinations varies widely at different loci and in cell types. Thus, disrupting genes polyploid lines or when using poorly performing sgRNAs require extensive downstream screening identify homozygous clones. Here we find that non-homologous single-stranded DNA greatly stimulates Cas9-mediated gene disruption...
Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology. Traditional isolation methods for peroxisomes can take more than 1 h to complete and be laborious implement. To address this, we have now extended our prior work on rapid organellar via the development peroxisomally localized 3XHA epitope tag ("PEROXO-Tag") associated immunoprecipitation ("PEROXO-IP") workflow. Our PEROXO-IP workflow has excellent reproducibility, is easy implement,...
In mammals, the cytosine in CG dinucleotides is typically methylated producing 5-methylcytosine (5mC), a chemically less stable form of that can spontaneously deaminate to thymidine resulting T•G mismatched base pair. Unlike other eukaryotes efficiently repair this pair back C•G, 5mCG deamination mutagenic, sometimes TG dinucleotides, explaining depletion mammalian genomes. It was suggested new generate genetic diversity may be critical for evolutionary change. We tested conjecture by...
The majority of genetic variants associated with common human diseases map to enhancers, non-coding elements that shape cell type-specific transcriptional programs and responses specific extracellular cues 1-3 . In order understand the mechanisms by which variation contributes disease, systematic mapping functional enhancers their biological contexts is required. Here, we develop an unbiased discovery platform can identify for a target gene without prior knowledge native context. We used...
ABSTRACT Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology. Traditional isolation methods for peroxisomes can take more than one hour to complete and be laborious implement. To address this, we have now extended our prior work on rapid organellar via the development peroxisomally-localized 3XHA epitope tag (“PEROXO-Tag”) associated immunoprecipitation (“PEROXO-IP”) workflow. Our PEROXO-IP workflow has excellent reproducibility, is...
Abstract Cas9 endonuclease can be targeted to genomic sequences by varying the sequence of single guide RNA (sgRNA). The activity these Cas9-sgRNA combinations varies widely at different loci and in cell types. Thus, disrupting genes polyploid lines, or using inefficient sgRNAs, require extensive downstream screening identify homozygous clones. We have found that linear, non-homologous oligonucleotide DNA greatly stimulates Cas9-mediated gene disruption absence homology-directed repair. This...