A5020699809- Glycogen Storage Diseases and Myoclonus
- Metabolism, Diabetes, and Cancer
- Genetics and Neurodevelopmental Disorders
- Plant nutrient uptake and metabolism
- Enzyme Production and Characterization
- Metabolism and Genetic Disorders
- Lysosomal Storage Disorders Research
- Plant Gene Expression Analysis
- Pancreatic function and diabetes
- Glycosylation and Glycoproteins Research
- Plant-Microbe Interactions and Immunity
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Microbial Metabolic Engineering and Bioproduction
- Plant Physiology and Cultivation Studies
- PI3K/AKT/mTOR signaling in cancer
- Plant Pathogens and Fungal Diseases
- Biofuel production and bioconversion
- Protein Tyrosine Phosphatases
University of Kentucky
2016-2021
Australian Research Council
2015
The University of Melbourne
2010-2015
SNF1-related protein kinase 1 (SnRK1) is the plant orthologue of evolutionarily-conserved SNF1/AMPK/SnRK1 family that contributes to cellular energy homeostasis. Functional as heterotrimers, members comprise a catalytic α subunit and non-catalytic β γ subunits; multiple isoforms each type exist, giving rise various isoenzymes. The Arabidopsis thaliana genome contains homologues type, and, in addition, two atypical subunits, β(3) βγ, with unique domain architecture, are found only amongst...
AMP‐activated protein kinase (AMPK) is a heterotrimer of catalytic (α) and regulatory (β γ) subunits with at least two isoforms for each subunit. AMPK β1 widely expressed whilst β2 highly in muscle both β contain mid‐molecule carbohydrate‐binding module (β‐CBM). Here we show that β2‐CBM has evolved to Thr insertion increased affinity glycogen mimetics preference oligosaccharides containing single α‐1,6 branched residue. Deletion Thr‐101 reduces by 3‐fold, while this residue into the...
The role of cellular metabolites in the direct control epigenetic modifications is an emerging and rapidly evolving field. Herein, we identify a key function for nuclear glycogen regulation through compartmentalized acetyl‐CoA production histone acetylation. In this study, report accumulation nucleus human non‐small cell lung cancer (NSCLC) specimens. To study striking phenotype, developed innovative assay combining high‐purity sub‐cellular organelle isolation stable isotope tracer...
Glycogen is the primary source of storage carbohydrate in mammals. synthesis and degradation either consumes or produces glucose‐6‐phosphate (G6P), a key metabolite essential for central carbon metabolism. Several studies have reported glycogen accumulation specific sub‐cellular organelles, suggesting that localization not random. Nuclear was first 1890s hepatocytes, subsequent reports identified near ER, mitochondria. Cumulatively, these data suggest compartment‐specific roles yet to be...
The growth of the bioethanol industry over last decade has been a result growing demand for gasoline fuels, which in United States constitutes at least 10% by volume, and implementation higher Renewable Fuel Standards (RFS). Bioethanol is produced fermentation simple sugars yeasts as starch feedstocks must undergo pretreatment steps. various mechanical, thermal chemical treatments used to degrade are both expensive energy intensive. Alternatively, plants use reversible phosphorylation...
The role of cellular metabolites in the direct control signaling is an emerging and rapidly evolving field. Herein, we identify a key for nuclear glycogen epigenetic regulation through compartmentalized acetyl CoA production histone acetylation. Nuclear glycogenolysis dependent on ubiquitination translocation phosphorylase (GP) into nucleus by malin, E3 ubiquitin ligase. We developed innovative organello stable isotope tracer method coupled to mass spectrometry analysis define metabolic fate...