A5088080829- Wildlife Ecology and Conservation
- Genetic diversity and population structure
- Genetic and phenotypic traits in livestock
- Species Distribution and Climate Change
- Rangeland and Wildlife Management
- Environmental DNA in Biodiversity Studies
- Animal Ecology and Behavior Studies
- Identification and Quantification in Food
- Ecology and Vegetation Dynamics Studies
- Molecular Biology Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Fish Ecology and Management Studies
- Animal Behavior and Reproduction
- Animal Behavior and Welfare Studies
- Ecology and biodiversity studies
- Plant and animal studies
- Forensic and Genetic Research
- Bat Biology and Ecology Studies
- Marine animal studies overview
- Amphibian and Reptile Biology
- Wildlife-Road Interactions and Conservation
- Avian ecology and behavior
- Conservation, Biodiversity, and Resource Management
- Genomics and Phylogenetic Studies
- Land Use and Ecosystem Services
University of Idaho
2016-2025
Animal and Plant Health Inspection Service
2005-2017
Utah State University
2005-2017
West Virginia University
2017
United States Fish and Wildlife Service
2005-2017
Centro Agronomico Tropical de Investigacion y Ensenanza Catie
2016
Amazon (United States)
2016
University of Wyoming
2014
Swiss Federal Institute for Forest, Snow and Landscape Research
2012
United States Department of Agriculture
2005
Our purpose was to identify an experimental procedure using PCR that provides a reliable genotype at microsatellite locus only few picograms of template DNA. Under these circumstances, it is possible (i) one allele heterozygous individual will not be detected and (ii) PCR-generated alleles or 'false alleles' arise. A mathematical model has been developed account for stochastic events when pipetting DNA in very dilute extract computer simulations have performed. Laboratory experiments were...
Abstract Individual identification using DNA fingerprinting methods is emerging as a critical tool in conservation genetics and molecular ecology. Statistical that estimate the probability of sampling identical genotypes theoretical equations generally assume random associations between alleles within among loci. These calculations are probably inaccurate for many animal plant populations due to population substructure. We evaluated accuracy identity ( P (ID) ) estimation by comparing...
Summary Species detection using environmental DNA ( eDNA ) has tremendous potential for contributing to the understanding of ecology and conservation aquatic species. Detecting species methods, rather than directly sampling organisms, can reduce impacts on sensitive increase power field surveys rare elusive The sensitivity however, requires a heightened awareness attention quality assurance control protocols. Additionally, interpretation data demands careful consideration multiple factors....
Environmental DNA (eDNA) methods for detecting aquatic species are advancing rapidly, but with little evaluation of field protocols or precision resulting estimates. We compared sampling results from traditional eDNA two amphibians in 13 streams central Idaho, USA. also evaluated three water collection and the influence location, time day, distance animals on concentration water. found no difference detection amount among protocols. had slightly higher rates than methods, particularly when...
Stream ecosystems harbor many secretive and imperiled species, studies of vertebrates in these systems face the challenges relatively low detection rates high costs. Environmental DNA (eDNA) has recently been confirmed as a sensitive efficient tool for documenting aquatic wetlands large river canal system. However, it was unclear whether this could be used to detect low-density fast-moving streams where shed cells may travel rapidly away from their source. To evaluate potential utility eDNA...
Abstract Pyrenean brown bears Ursus arctos are threatened with extinction. Management efforts to preserve this population require a comprehensive knowledge of the number and sex remaining individuals their respective home ranges. This goal has been achieved using combination noninvasive genetic sampling hair faeces collected in field corresponding track size data. Genotypic data were at 24 micrdsatellite loci rigorous multiple‐tubes approach avoid genotyping errors associated low quantities...
Environmental DNA (eDNA) methods for detecting and estimating abundance of aquatic species are emerging rapidly, but little is known about how processes such as secretion rate, environmental degradation, time since colonization or extirpation from a given site affect eDNA measurements. Using stream-dwelling salamanders quantitative PCR (qPCR) analysis, we conducted three experiments to assess eDNA: (i) production rate; (ii) persistence under different temperature light conditions; (iii)...
The 196 parties to the Convention on Biological Diversity (CBD) will soon agree a post-2020 global framework for conserving three elements of biodiversity (genetic, species, and ecosystem diversity) while ensuring sustainable development benefit sharing. As most significant conservation policy mechanism, new CBD has far-reaching consequences- it guide actions reporting each member country until 2050. In previous strategies, as well other major mechanisms, targets indicators genetic diversity...
Abstract A large microsatellite data set from three species of bear (Ursidae) was used to empirically test the performance six genetic distance measures in resolving relationships at a variety scales ranging adjacent areas continuous distribution that diverged several million years ago. At finest scale, while some performed extremely well, statistics developed specifically accommodate mutational processes microsatellites relatively poorly, presumably because higher variance these statistics....
Early detection of aquatic invasive species is a critical task for management ecosystems. This hindered by the difficulty and cost surveying systems thoroughly. The New Zealand mudsnail (Potamopyrgus antipodarum) small, parthenogenic mollusk that can reach very high population densities severely affects ecosystem functioning. To assist in early this species, we developed validated highly sensitive environmental deoxyribonucleic acid (eDNA) assay. We used dose–response laboratory experiment...
The goal of landscape genetics is to detect and explain effects on genetic diversity structure. Despite the increasing popularity approaches, statistical methods for linking data remain largely untested. This lack method evaluation makes it difficult compare studies utilizing different statistics, compromises future development application field. To investigate suitability comparability various approaches used in genetics, we simulated sets corresponding five landscape‐genetic scenarios. We...
Microsatellite variability is widely used to infer levels of genetic diversity in natural populations. However, the ascertainment bias caused by typically selecting only most polymorphic markers genome may lead reduced sensitivity for judging genome-wide diversity. To test this potential limitation microsatellite-based approaches, we assessed degree nucleotide noncoding regions eight different carnivore populations, including inbred as well outbred sequencing 10 introns (5.4-5.7 kb) 20...
A growing number of population genetic studies utilize nuclear DNA microsatellite data from museum specimens and noninvasive sources. Genotyping errors are elevated in these low quantity sources, potentially compromising the power accuracy data. The most conservative method for addressing this problem is effective, but requires extensive replication individual genotypes. In search a more efficient method, we developed maximum-likelihood approach that minimizes by estimating genotype...
Abstract The use of non‐invasive genetic sampling to estimate population size in elusive or rare species is increasing. data generated from this differ traditional mark–recapture that individuals may be captured multiple times within a session there only single event. To accommodate type data, we develop method, named capwire , based on simple urn model containing two capture probabilities. method evaluated using simulations an and more biologically realistic system where occupy space,...
Understanding the factors that influence rate at which natural populations lose genetic diversity is a central aspect of conservation genetics because importance in maintaining evolutionary potential and individual fitness. Concerns about loss are particularly relevant to large carnivores, such as brown bears (Ursus arctos), distributed low densities highly susceptible human‐caused population fragmentation. We used eight variable nuclear microsatellite markers study current levels variation...
Abstract In the 1930s, Scandinavian brown bear was close to extinction due vigorous extermination programmes in Norway and Sweden. Increased protection of Scandinavia has resulted recovery four subpopulations, which currently contain 1000 individuals. Effective conservation management requires knowledge current levels genetic diversity gene flow among subpopulations. Earlier studies mitochondrial DNA (mtDNA) revealed extremely low variation, population structure that grouped three northern...
To test whether plucked hairs are a reliable source of DNA for genotyping microsatellite loci, we carried out experiments using one, three, or 10 per extract 50 alpine marmots. For each extract, seven independent genotypings were performed the same locus (multiple‐tubes approach). Two types errors recorded: false homozygote defined as detection only one allele true heterozygote, and PCR‐generated that was not alleles genotype. Using extracted from hairs, overall error rate 14.00%, 4.86%,...