A5018434112- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Fungal Biology and Applications
- Fungal and yeast genetics research
- Microbial Metabolites in Food Biotechnology
- Plant Pathogens and Fungal Diseases
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Enzyme-mediated dye degradation
- Plant-Microbe Interactions and Immunity
- Mycorrhizal Fungi and Plant Interactions
- Hemoglobinopathies and Related Disorders
- Enzyme Catalysis and Immobilization
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Plant Disease Resistance and Genetics
- Transgenic Plants and Applications
- Microbial Metabolism and Applications
- Antioxidant Activity and Oxidative Stress
- Plant nutrient uptake and metabolism
- Autoimmune Bullous Skin Diseases
- Plant biochemistry and biosynthesis
- Salivary Gland Disorders and Functions
- Genetics, Bioinformatics, and Biomedical Research
- Evolutionary Algorithms and Applications
University of Toronto
1991-2000
Novo Nordisk (United States)
1999
Novo Nordisk (Denmark)
1995
Aurora College
1993
SUNY College of Environmental Science and Forestry
1983-1992
State University of New York
1983-1992
Institut Gustave Roussy
1992
Université Claude Bernard Lyon 1
1991-1992
Centre de Génétique Moléculaire
1992
Institute for Biological Sciences
1992
Abstract This study followed the transmission of 64 segregating genetic markers to 52 haploid offspring, obtained from both homokaryotic and heterokaryotic meiospores, a cross (AG 93b) Agaricus bisporus, commonly cultivated "button mushroom." The electrophoretic karyotypes AG 93b component nuclei were determined concurrently (n = 13). Eleven distinct linkage groups identified by two-point analysis. DNA-DNA hybridization showed that nine these corresponded unique chromosome-sized DNAs. Two...
Xylose oligomers rapidly induced xylanase activity of Trichoderma longibrachiatum, whereas induction was delayed in the presence glucose. Cellobiose, cellopentaose, and xylobiose did not induce detectable levels cellulase activity. However, mixtures with cellobiose or cellopentaose In addition, more effectively than alone. Both were detected after a lag period lactose.
A laminarinase [endo-(1,3)-β- d -glucanase] has been purified from Trichoderma longibrachiatum cultivated with -glucose as the growth substrate. The enzyme was found to hydrolyze laminarin oligosaccharides varying in size glucose pentaose and lesser amounts of larger oligosaccharides. unable cleave laminaribiose but hydrolyzed triose glucose. cleaved laminaritetraose, yielding laminaritriose, laminaribiose, glucose, similarly laminaripentaose, only glucans containing β-1,3 linkages. pH...
Two endoxylanases were purified from the culture medium of Trichoderma longibrachiatum . Both enzymes highly basic, and lacked activity on carboxymethyl‐cellulose. An enzyme 21.5 kDa (xylanase A) had a specific 510 U/mg protein, K m 0.15 mg soluble xylan/ml, possessed transglycosidase generated xylobiose xylotriose as major endproducts xylan or xylose oligomers. A larger 33 B) 131 0.19 detectable Xylotriose was smallest oligomer attacked by both enzymes. In addition, inhibited hydrolysis...
A method capable of detecting as little 0.11 U xylanase activity in polyacrylamide gels was developed. The entails incubation protein contact with substrate containing unmodified xylan, followed by immersion 95% ethanol. Resulting zymograms contain transparent bands corresponding to enzymatic against an opaque background.
An efficient procedure is presented for the generation of protoplasts from shake flask cultures commercial mushroom Agaricus bisporus. Orthogonal field electrophoresis (OFAGE) high molecular weight DNA lysed resolved genome A. bisporus into at least 10 bands, ranging in size 1.2 to 4 Mb. The illustrated electrophoretic karyotypes two homokaryons were highly polymorphic. A heterokaryon contained a mixture patterns, though ratio nuclear types was not equal. number RFLP and RAPD markers rDNA...
Rhodoxanthin is a vibrant red carotenoid found across the plant kingdom and in certain birds fish. It member of atypical retro class carotenoids, which contain an additional double bond concerted shift conjugated bonds relative to more widely occurring pigments, whose biosynthetic origins have long remained elusive. Here, we identify LHRS (Lonicera hydroxylase rhodoxanthin synthase), variant β-carotene (BCH)-type integral membrane diiron enzyme that mediates conversion into rhodoxanthin. We...
We have isolated an erythropoiesis-inhibiting protein, DIP (differentiation-inhibiting protein), from the blood of a 60-year-old woman suffering pure red cell aplasia. This protein inhibits growth and differentiation normal human murine BFU-E, but not CFU-E, cells as well dimethyl sulfoxide-induced hemoglobin synthesis by Friend erythroleukemia cells. It appears that primarily affects rather than proliferation, because it does inhibit proliferation untreated seems to function like recently...