Xu Cai

ORCID: 0000-0002-1370-2891
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About
Contact & Profiles
Research Areas
  • Chromosomal and Genetic Variations
  • Genomics and Phylogenetic Studies
  • Plant Disease Resistance and Genetics
  • Nitrogen and Sulfur Effects on Brassica
  • Plant tissue culture and regeneration
  • Plant Virus Research Studies
  • Plant Reproductive Biology
  • Plant Molecular Biology Research
  • Cancer-related molecular mechanisms research
  • Plant Ecology and Taxonomy Studies
  • Photosynthetic Processes and Mechanisms
  • Plant Taxonomy and Phylogenetics
  • Genetic diversity and population structure
  • Lipid metabolism and biosynthesis
  • HER2/EGFR in Cancer Research
  • Genomics and Chromatin Dynamics
  • RNA and protein synthesis mechanisms
  • Remote Sensing and Land Use
  • Genomics, phytochemicals, and oxidative stress
  • Aquatic Ecosystems and Biodiversity
  • Natural product bioactivities and synthesis
  • Plant Gene Expression Analysis
  • Water-Energy-Food Nexus Studies
  • Plant Stress Responses and Tolerance
  • Mast cells and histamine

Institute of Vegetables and Flowers
2017-2025

Chinese Academy of Agricultural Sciences
2017-2025

Guizhou University
2024

Children's Hospital of Chongqing Medical University
2023

Chongqing Medical University
2023

Army Medical University
2023

The Affiliated Yongchuan Hospital of Chongqing Medical University
2023

Institute of Oceanology
2015

Liaoning University of Traditional Chinese Medicine
2013

Shandong Agricultural University
2012

Brassica rapa comprises several important cultivated vegetables and oil crops. Current reference genome assemblies of are quite fragmented not highly contiguous, thereby limiting extensive genetic genomic analyses. Here, we report an improved assembly the B. (v3.0) using single-molecule sequencing, optical mapping, chromosome conformation capture technologies (Hi-C). Relative to previous genomes, our features a contig N50 size 1.45 Mb, representing ~30-fold improvement. We also identified...

10.1038/s41438-018-0071-9 article EN cc-by Horticulture Research 2018-07-24

Abstract The Brassicaceae Database (BRAD version 3.0, BRAD V3.0; http://brassicadb.cn) has evolved from the former Brassica V2.0), and represents an important community portal hosting genome information for multiple related plant species. Since last update in 2015, complex genomes of numerous species have been decoded, accompanied by many omics datasets. To provide up-to-date service, we report here a major upgrade portal. Model-View-ViewModel (MVVM) framework re-engineered to enable easy...

10.1093/nar/gkab1057 article EN cc-by Nucleic Acids Research 2021-10-19

Despite the prevalence and recurrence of polyploidization in speciation flowering plants, its impacts on crop intraspecific genome diversification are largely unknown. Brassica rapa is a mesopolyploid species that domesticated into many subspecies with distinctive morphotypes.Herein, we report consequences whole-genome triplication (WGT) using pan-genome analysis 16 de novo assembled two reported genomes. Among genes derive from WGT, 13.42% polyploidy-derived accumulate more transposable...

10.1186/s13059-021-02383-2 article EN cc-by Genome biology 2021-05-31

Transposable element (TE) is prevalent in plant genomes. However, studies on their impact phenotypic evolution crop plants are relatively rare, because systematically identifying TE insertions within a species has been challenge. Here, we present novel approach for uncovering insertion polymorphisms (TIPs) using pan-genome analysis combined with population-scale resequencing, and adopt this pipeline to retrieve TIPs Brassica rapa germplasm collection. We found that 23% of genes the reference...

10.1111/pbi.13807 article EN Plant Biotechnology Journal 2022-03-12

The species Brassica rapa includes several important vegetable crops. draft reference genome of B. ssp. pekinensis was completed in 2011, and it has since been updated twice. pangenome with structural variations 18 accessions published 2021. Although extensive genomic analysis conducted on rapa, a comprehensive annotation including gene structure, alternative splicing (AS) events, non-coding genes is still lacking. Therefore, we used the Pacific Biosciences (PacBio) single-molecular...

10.3389/fpls.2022.841618 article EN cc-by Frontiers in Plant Science 2022-03-17

Energy and food source crop demand claims to be vulnerable climate change impacts. The new orphan crops, which in the past have received only limited research attention but are sustainable environmental systems, needed. In this review, we summarize available literature about Ethiopian mustard as an alternative energy its economic importance a promising Brassicacea for opportunities face of producing environment development. has many advantages can adopted replace crops that susceptible...

10.3390/su12187492 article EN Sustainability 2020-09-11

Abstract The Brassicaceae family includes many economically important crop species, as well cosmopolitan agricultural weed species. In addition, Arabidopsis thaliana, a member of this family, is used molecular model plant genus Brassica mesopolyploid, and the comprises comparatively recently originated tetrapolyploid With these characteristics, Brassicas have achieved commonly accepted status organisms for genomic studies. This paper reviews rapid research progress in from diverse omics...

10.1093/hr/uhac182 article EN cc-by Horticulture Research 2022-01-01

Brassica species evolved through recurrent polyploidization and chromosomal rearrangements, forming diploid progenitors that hybridize into allopolyploids. These plants exhibit remarkable morphological diversity, with specialized edible organs including leaf-, stem-, root-, oil-type cultivars, yet cross-species multi-organ transcriptomic studies elucidating their gene expression similarities divergences remain lacking. To address this gap, we analyzed publicly available transcriptomes...

10.3390/horticulturae11040427 article EN cc-by Horticulturae 2025-04-16

Abstract The leafy head characteristic is a special phenotype of Chinese cabbage resulting from artificial selection during domestication and breeding. BREVIS RADIX ( BRX ) has been suggested to control root elongation, shoot growth, tiller angle in Arabidopsis rice. In Brassica rapa , three BrBRX homoeologs have identified, but only BrBRX.1 BrBRX.2 were found be under leaf-heading accessions, indicating their functional diversification formation. Here, we show that these genes belong...

10.1038/s41438-021-00504-3 article EN cc-by Horticulture Research 2021-04-01

Brassica rapa is one of the most important leafy vegetable crops with large cultivated area in China. To increase availability DNA markers B. rapa, we developed insertion-deletion (InDel) utilizing high-resolution melting (HRM) curve analysis. We designed primers for 252 InDels (≥ 3 bp) evenly distributed genome and tested gene polymorphisms eight accessions. In total, 208 were specifically amplified, 148 polymorphism genotyped successfully using HRM. further analyzed correlation InDel size,...

10.1016/j.hpj.2020.05.003 article EN cc-by-nc-nd Horticultural Plant Journal 2020-05-23

Ethiopian mustard (Brassica carinata) is mainly grown as a leafy vegetable and oilseed crop. Sinigrin predominant glucosinolate in the leaves of mustard. It hydrolyzed by enzyme myrosinase to produce bioactive product specifically allyl-isothiocyanates. Due sinigrin content, has mild flavor it healthy addition human diet. However, concentration depends on genotype. Understanding variations plant morphology content may be first step for crop improvement. According analyses various...

10.1016/j.hpj.2019.07.005 article EN cc-by-nc-nd Horticultural Plant Journal 2019-07-30

Long terminal repeat retrotransposons (LTR-RTs) are a predominant group of plant transposable elements (TEs) that an important component genomes. A large number LTR-RTs have been annotated in the genomes agronomically oil and vegetable crops genus Brassica. Herein, full-length Brassica other closely related species were systematically analyzed. The LTR-RT content varied greatly (from 0.43% to 23.4%) between different species, with Gypsy-like constituting primary across these More...

10.1016/j.hpj.2018.05.002 article EN cc-by-nc-nd Horticultural Plant Journal 2018-06-06

Abstract High-throughput Chromatin Conformation Capture (Hi-C) technologies can be used to investigate the three-dimensional genomic structure of plants. However, practical utility these is impeded by significant background noise, hindering their capability in detecting fine 3D structures. In this study, we optimized Bridge Linker Hi-C technology (BL-Hi-C) comprehensively chromatin landscape Brassica rapa and oleracea. The Bouquet configuration both B. oleracea was elucidated through...

10.1093/hr/uhae017 article EN cc-by Horticulture Research 2024-01-16

Self-incompatibility (SI), which has recurred during the evolution of plants, is one most important cross-pollination mating systems. Three S-loci have been reported in Brassicaceae, namely, Arabidopsis lyrata (Al), Brassica (Br), and Leavenworthia alabamica (La) S-loci. Here, through multi-genomic comparative analysis 20 species, we revealed that ancient S-locus was formed prior to divergence Brassicaceae lineage I II. It retained inherited by Arabidopsis, as Al I. Furthermore, found Br...

10.1016/j.hpj.2020.04.005 article EN cc-by-nc-nd Horticultural Plant Journal 2020-05-01

The species Brassica rapa includes enormous leafy vegetables with extreme leaf morphological diversity. Leaf traits such as size, shape, weight, and ratio of the blade to petiole contribute yield, appearance, desirability consumers. These leaf-related are controlled by quantitative trait loci (QTLs). construction high-density bin maps using low-coverage sequencing is a powerful method for QTL fine-mapping gene identification. In this study, we performed whole-genome re-sequencing Wutacai...

10.3390/horticulturae9040433 article EN cc-by Horticulturae 2023-03-27

Flowering time is important for Brassica rapa vegetables because premature bolting before harvest can lower yield and quality. FLOWERING LOCUS C (FLC) acts as a key repressor of flowering. In this study, we identified nonsynonymous mutation at the 58th nucleotide exon1 in BrFLC1 (named Pe1+58 (A/C)) by screening resequencing data 199 B. accessions verified being related to flowering variation. Strong linkage inheritance was detected between locus previously reported splicing site intron 6...

10.3390/horticulturae7080247 article EN cc-by Horticulturae 2021-08-14
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