A5006488612- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Amino Acid Enzymes and Metabolism
- Protein Structure and Dynamics
- Inflammatory mediators and NSAID effects
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Electron Spin Resonance Studies
- Lanthanide and Transition Metal Complexes
- Porphyrin Metabolism and Disorders
- Hemoglobin structure and function
- Mitochondrial Function and Pathology
- Eicosanoids and Hypertension Pharmacology
- Metabolism and Genetic Disorders
- Biochemical and Molecular Research
- ATP Synthase and ATPases Research
- Spectroscopy and Quantum Chemical Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Magnetism in coordination complexes
- RNA and protein synthesis mechanisms
- Nitric Oxide and Endothelin Effects
- Microbial Inactivation Methods
- Estrogen and related hormone effects
- Receptor Mechanisms and Signaling
- Algal biology and biofuel production
Institut de Biologie Physico-Chimique
2007-2022
Université Paris Cité
2005-2022
Centre National de la Recherche Scientifique
1999-2022
Laboratoire de Biologie Physico-Chimique des Protéines Membranaires
2010-2017
Sorbonne Université
1996-2016
Délégation Provence et Corse
2012
Aix-Marseille Université
2012
Délégation Paris 7
2008
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2005
École Nationale Supérieure des Mines de Paris
1998
The cyclooxygenase activity of the membrane protein prostaglandin H2 synthase isoform 1 (PGHS-1) is target nonsteroidal antiinflammatory drugs (NSAIDs). X-ray crystal structures PGHS-1 in complex with NSAIDs flurbiprofen and bromoaspirin have been determined previously [Picot, D., et al. (1994) Nature 367, 243−249; Loll, P. J., (1995) Nat. Struct. Biol. 2, 637−643]. We report here preparation characterization novel potent iodinated analogs indomethacin suprofen, as well refined their...
Absorption spectra of the purified cytochrome b 6 f complex from Chlamydomonas reinhardtii were monitored as a function redox potential. Four spectral and components identified: in addition to heme two hemes, fourth component must be new c i (also denoted x ) recently discovered crystallographic structures. This is covalently attached protein, but has no amino acid axial ligand. It located plastoquinone-reducing site Q immediate vicinity heme. Each titrated one-electron Nernst curve, with...
DNase I requires Ca2+ and Mg2+ for hydrolyzing double-stranded DNA. However, the number location of ion-binding sites remain unclear, as well role these counter-ions. Using molecular dynamics simulations, we show that bovine pancreatic (bp) contains four pockets. Two them strongly bind while other two coordinate Mg2+. These theoretical results are supported by revisiting crystallographic structures contain bpDNase I. One stabilizes functional structure. The presence in close vicinity to...
X-band EPR features in the region of 90–150 mT have previously been attributed to heme c i b 6 complex [Zhang H, Primak A, Bowman MK, Kramer DM, Cramer WA (2004) Biochemistry 43:16329–16336] and interpreted as arising from a high-spin species. However, complexity observed spectrum is rather untypical for hemes. In this work, we show that addition inhibitor 2- n -nonyl-4-hydroxyquinoline N -oxide largely simplifies 's properties. The presence demonstrated be caused by simple S = 5/2, rhombic...
Mixed protein-surfactant micelles are used for in vitro studies and 3D crystallization when solutions of pure, monodisperse integral membrane proteins required. However, many undergo inactivation transferred from the biomembrane into conventional surfactants with alkyl chains as hydrophobic moieties. Here we describe development rigid, saturated or aromatic hydrocarbon groups parts. Their stabilizing properties demonstrated three different proteins. The temperature at which 50% binding sites...
Abstract Strategies to harness photosynthesis from living organisms generate electrical power have long been considered, yet efficiency remains low. Here, we aimed reroute photosynthetic electron flow in without compromising their phototrophic properties. We show that 2,6-dimethyl- p -benzoquinone (DMBQ) can be used as an mediator assess the of mutations designed engineer a novel donation pathway downstream primary acceptor Q A Photosystem (PS) II green alga Chlamydomonas reinhardtii ....
Aspartate aminotransferase undergoes major shifts in the conformational equilibrium of protein matrix during transamination. The present study defines two states enzyme by crystallographic analysis, examines conditions under which crystallizes each these conformations, and correlates with behaviour solution, as monitored a fluorescent reporter group. Cocrystallization chicken mitochondrial aspartate inhibitors covalent coenzyme‐substrate adducts yields three different crystal forms....