A5015556667- Bacillus and Francisella bacterial research
- Biosensors and Analytical Detection
- Genomics and Rare Diseases
- Genomic variations and chromosomal abnormalities
- Genetic Associations and Epidemiology
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
Perlegen (United States)
2005
Portable detection and quantitation methods for Bacillus anthracis (anthrax) spores in pure culture or environmental samples are lacking. Here, an amperometric immunoassay has been developed utilizing immunomagnetic separation to capture the remove potential interferents from test followed by measurement on a field-portable instrument. Antibody-conjugated magnetic beads antibody-conjugated glucose oxidase were used sandwich format of target spores. Glucose activity spore pellets was measured...
Antibody-based detection assays are generally robust, a desirable characteristic for in-the-field use. However, to quantify the colorimetric or fluorescent signal, these require expensive and fragile instruments which ill-suited Lateral flow devices (LFDs) circumvent barriers portability but suffer from poor sensitivity subjective interpretation. Here, an antibody-based method detecting Bacillus anthracis spores via amperometric signal generation is compared ELISA LFDs. This immunoassay uses...