A5024251898- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Advanced Polymer Synthesis and Characterization
- Layered Double Hydroxides Synthesis and Applications
- Monoclonal and Polyclonal Antibodies Research
- Dendrimers and Hyperbranched Polymers
- Lipid Membrane Structure and Behavior
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Sarcoma Diagnosis and Treatment
- Copper-based nanomaterials and applications
- Luminescence and Fluorescent Materials
- Analytical Chemistry and Chromatography
- Polymer Surface Interaction Studies
- Pancreatic function and diabetes
- Electrospun Nanofibers in Biomedical Applications
- Aerogels and thermal insulation
- Occupational and environmental lung diseases
- ZnO doping and properties
- Mesoporous Materials and Catalysis
- Viral Infections and Immunology Research
- Calcium Carbonate Crystallization and Inhibition
- Bone Tissue Engineering Materials
- Tea Polyphenols and Effects
Eli Lilly (United States)
2024
University of Massachusetts Amherst
2017-2022
Corteva (United States)
2021
United States Military Academy
2020
Amherst College
2019
Unilever (India)
2011-2013
Trafficking proteins inside cells is an emerging field with potential utility in basic cell biology and biological therapeutics. A robust sustainable delivery strategy demands not only good protection of the cargo but also reversibility conjugation activity. We report a protein-templated polymer self-assembly for forming sheath around then tracelessly releasing them cytosol. The versatility approach, demonstrated here, suggests that compatible wide array biologics.
Endosomal entrapment has remained the major bottleneck for cytosolic delivery of nanoparticle-based systems. Uncovering fundamentally new pathways endosomal escape is therefore highly sought. Herein, we report that disulfide bonds can enhance through contacts with cellular exofacial thiols, in addition to facilitating uptake. Our results are supported comparative analysis polymeric nanogels variable accessibility by placing these functionalities at core or shell nanogels. The findings here...
Due to their relatively large molecular sizes and delicate nature, biologic drugs such as peptides, proteins, antibodies often require high repeated dosing, which can cause undesired side effects physical discomfort in patients render many therapies inordinately expensive. To enhance the efficacy of drugs, they could be encapsulated into polymeric hydrogel formulations preserve stability help tune release body most favorable profile action for a given therapy. In this study, series...
The effect of citrate as a crystal habit modifier on the morphology zinc oxide has been explored in wide range citrate/Zn ratio from 0 to 0.67. With an increase concentration at lower ratio, aspect hexagonal prismatic crystals ZnO decreases, forming flatter disks with progressive roughness face. At intermediate ratios, otherwise smooth rectangular faces also become rough, formation porous spherical aggregates. high citrate, solid particles form very low crystallinity, eventually plate-like...
The primary impediments in developing large antibodies as drugs against intracellular targets involve their low transfection efficiency and suitable reversible encapsulation strategies for delivery with retention of biological activity. To address this, we outline an electrostatics-enhanced covalent self-assembly strategy to generate polymer-protein/antibody nanoassemblies. Through structure-activity studies, down-select the best performing self-immolative pentafluorophenyl containing...
Nanocarrier-mediated drug delivery is a promising strategy to maximize the power of chemotherapy and minimize side effects. However, current approaches show insufficient drug-loading capacity inefficient release, require complex modification processes. Attempts enhance one these features often compromise other merits. We describe here block copolymer assembly system that combines desirable characteristics. The design self-immolative crosslinkable hydrophobic moieties offer stable high...
Nucleic acids are now considered as one of the most potent therapeutic modalities, their roles go beyond storing genetic information and chemical energy or signal transducer. Attenuation expression desired genes through nucleic have profound implications in gene therapy, editing even vaccine development for immunomodulation. Although acid therapeutics bring overwhelming possibilities towards molecular medicines, there significant loopholes designing effective translation these drugs into...
A novel cone-shaped superhydrophobic and oleophilic CaAl-LDH intercalated with dodecyl sulphate (DS(-)) anions was synthesized from calcium as a precursor via one-step hydrothermal method. The LDH has been successfully utilized in mopping regeneration of oil oil-water mixtures.
This paper demonstrates crystallization of calcium carbonate in the presence insoluble Mg–Al layered double hydroxides (LDH). The metastable aragonite phase is preferentially stabilized over thermodynamically more stable calcite this inorganic matrix at ambient temperature. Such particles possess an unconventional "rosette-like" morphology. In intermediate stages crystallization, another phase, vaterite, having a "flaky-floret-like" morphology, initially formed, and unusual transformation...
Disulfide cross-linked nanoassemblies have attracted considerable attention as a drug delivery vehicle due to their responsiveness the natural redox gradient in biology. Fundamentally understanding factors that influence loading capacity, encapsulation stability, and precise control of liberation encapsulated cargo would be profoundly beneficial redox-responsive materials. Reported herein are block copolymer (BCP)-based self-cross-linked nanogels, which exhibit high controllable release...
A novel "symbiotic self-assembly" strategy that integrates the advantages of biocompatible lipids with a structurally robust polymer to efficiently encapsulate and deliver siRNAs is reported. The assembly process considered be symbiotic because none assembling components are capable self-assembly but can form well-defined nanostructures in presence others. conditions simple have been chosen such it offers ability arrive at system noncationic for mitigating carrier-based cytotoxicity, siRNA...
Abstract The primary impediments in developing large antibodies as drugs against intracellular targets involve their low transfection efficiency and suitable reversible encapsulation strategies for delivery with retention of biological activity. To address this, we outline an electrostatics‐enhanced covalent self‐assembly strategy to generate polymer‐protein/antibody nanoassemblies. Through structure–activity studies, down‐select the best performing self‐immolative pentafluorophenyl...
Abstract Endosomal entrapment has remained the major bottleneck for cytosolic delivery of nanoparticle‐based systems. Uncovering fundamentally new pathways endosomal escape is therefore highly sought. Herein, we report that disulfide bonds can enhance through contacts with cellular exofacial thiols, in addition to facilitating uptake. Our results are supported comparative analysis polymeric nanogels variable accessibility by placing these functionalities at core or shell nanogels. The...
Peroxide-mediated oxidation of drug molecules is a known challenge faced throughout the pharmaceutical development pathway-from early-stage stability studies to manufacturing processes. During initial stage, major source peroxide formulation excipients, whether they are pre-loaded or generated in situ due slow degradation, and late phase, peroxides can be introduced during sanitization processes via cavitation. In essence, control strategy for mitigation often becomes critical quality...
Abstract Nanocarrier‐mediated drug delivery is a promising strategy to maximize the power of chemotherapy and minimize side effects. However, current approaches show insufficient drug‐loading capacity inefficient release, require complex modification processes. Attempts enhance one these features often compromise other merits. We describe here block copolymer assembly system that combines desirable characteristics. The design self‐immolative crosslinkable hydrophobic moieties offer stable...
The positional effect of stimuli-responsive units in tri-component copolymer vesicles is studied to explore variations the host–guest properties assembly.