A5057471395- Isotope Analysis in Ecology
- Soil Carbon and Nitrogen Dynamics
- Marine and coastal ecosystems
- Plant Growth Enhancement Techniques
- Geology and Paleoclimatology Research
- Composting and Vermicomposting Techniques
- Radioactive contamination and transfer
- Radioactive element chemistry and processing
- Clay minerals and soil interactions
- NMR spectroscopy and applications
- Lignin and Wood Chemistry
- Heavy metals in environment
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Molecular Sensors and Ion Detection
- Various Chemistry Research Topics
- Allelopathy and phytotoxic interactions
- Aquatic and Environmental Studies
- Peatlands and Wetlands Ecology
- Marine and coastal plant biology
- Lichen and fungal ecology
- Biochemical and biochemical processes
- Molecular Junctions and Nanostructures
- Microbial Fuel Cells and Bioremediation
- Geochemistry and Elemental Analysis
University of Naples Federico II
2007-2016
Institute for Coastal Marine Environment
2014
We propose a mild stepwise fractionation of molecular components humic acid (HA) suprastructure and their structural identification by advanced analytical methods. This procedure may be the basis "Humeomics" approach to characterize natural molecules clarify relations with ecosystems functions. Sequential included: (1) organic solvent extraction, (2) transesterification boron trifluoride in methanol (BF(3)-CH(3)OH), (3) methanolic alkaline hydrolysis (KOH-CH(3)OH), (4) cleavage ether...
We progressively reduced the complexity of humic matter by a mild sequential removal unbound or free components, weakly, and strongly bound molecules. The auxin-like response residues from each step was tested using tomato (cv. Micro-Tom) seedlings expressing DR5 auxin synthetic promoter fused to β-glucuronidase (GUS) reporter gene low auxin-sensitivity diageotropica (dgt) mutant. Both exogenous promoted lateral root emergence in control, but failed induce roots dgt When components were...
Abstract Background The effects of a humic substance (HS) extracted from volcanic soil on the nitrate assimilation pathway Zea mays seedlings were thoroughly examined using physiological and molecular approaches. Plant growth, amount soluble proteins amino acids, as well activities enzymes involved in nitrogen metabolism Krebs cycle, evaluated response to different HS concentrations (0, 1 5 mg C L −1 ) supplied maize for 48 h. To better understand action, transcript accumulation selected...
The molecular composition of water-soluble lignins isolated from four non-food bioenergy crops (cardoon CAR, eucalyptus EUC, and two black poplars RIP LIM) was characterized in detail, their potential bioactivity towards maize germination early growth evaluated. Lignins were found to not affect seed rates, but stimulated the seedling development, though a different extent. promoted root elongation, while CAR only length lateral seminal roots coleoptile, LIM improved coleoptile development....
NMR techniques were applied to follow changes in molecular rigidity and diffusion of complexes formed between a humic acid (HA) either aluminum or calcium ions, added amounts ranging from 0.05% 1% HA carboxylic acidity. Spin-lattice relaxation time the rotating frame (T1rho,(H)) coefficients (D) humic-metal obtained 13C cross-polarization magic angle spinning (CP-MAS), 1H-diffusion order spectroscopy (DOSY) spectra, respectively. Molecular increased significantly with metal addition...
The current wide diffusion of bio-degradable plastic made up by starch-based polymeric composite has focused the attention on allocation bio-polymers for direct recycling in composting processes. Actually, acknowledged methods to estimate bio-degradability are mainly based laboratory tests and measurements under controlled conditions, while scarce information available effective transformation bio-film derivatives real facilities. aim this paper was determine at molecular level decomposition...
Here we describe an innovative mechanism for carbon sequestration in soil by situ photopolymerization of organic matter under biomimetic catalysis. Three different Mediterranean soils were added with a synthetic water-soluble iron-porphyrin, irradiated solar light, and subjected first to 5 days incubation and, then, 15, 30 wetting drying (w/d) cycles. The catalyst-assisted (SOC) increased water stability aggregates both after 15 w/d cycles, but not Particle-size distribution all treated...
Humeomics encompasses step-wise chemical fractionation and instrumental determination to fully characterize the heterogeneous molecular composition of natural organic matter.
Humeomics is a sequential step-wise chemical fractionation that simplifies the complex matrix of humic acid (HA) and weakens its supramolecular interactions, thereby allowing detailed characterization involved molecules. A recalcitrant residual end product Humeomics, namely RES4, was successfully solubilized here in alkaline conditions subjected to semi-preparative high-performance size exclusion chromatography (HPSEC). The resulting six fractions separated by HPSEC were analyzed different...
A model heterogeneous mixture of a hydrophilic tripeptide (phenylalanine-glycine-glycine, PGG) and hydrophobic organic acids ((12)C- (13)C-octanoic acid pentadecanoic acid) was subjected to electrospray ionization mass spectrometry (ESI-MS). The objective verify the previously noted inconsistencies in ESI-MS complex environmental samples such as humic materials from either aquatic or terrestrial origins. acids, alone together, reduced significantly detection molecular self-associated ions at...
In light of the growing interest for eco-compatible fertilization, tomato plant roots were treated with four different strains microorganisms (B1–B4) capable positively affecting growth. The methanolic extracts from analysed by reverse phase ultra-high-performance liquid chromatography hyphenated ion trap time-of-flight high-resolution mass spectrometry to compare their metabolites that control plants (B0). We found, in both and plants, several primary metabolites, such as fatty acids...
In this work, humic (HA) and fulvic acid (FA) were chemically modified by esterification etherification with alkanes under microwave (MW) irradiation to improve their surfactant properties for the remediation of total petroleum hydrocarbons (TPHs)-contaminated soil. Humic FA evaluated as soil means washing an aged highly TPH-contaminated (50,000 mg TPH kg) sampled from a Mexican petrochemical area. The efficiency chemical modification HA was increased accelerated MW respect that conventional...