A5028389456- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Enzyme Production and Characterization
- Helicobacter pylori-related gastroenterology studies
- Cancer therapeutics and mechanisms
- Liver Disease Diagnosis and Treatment
- Microbial Natural Products and Biosynthesis
- DNA and Nucleic Acid Chemistry
- Protein Hydrolysis and Bioactive Peptides
- Gastric Cancer Management and Outcomes
- Enzyme Catalysis and Immobilization
- Angiogenesis and VEGF in Cancer
- Chemical Synthesis and Analysis
- Carbohydrate Chemistry and Synthesis
- Synthetic Organic Chemistry Methods
- Music Technology and Sound Studies
- Synthesis and Biological Evaluation
- Bioactive Compounds and Antitumor Agents
- Drug Transport and Resistance Mechanisms
- Gastrointestinal disorders and treatments
- Organometallic Complex Synthesis and Catalysis
- Synthesis and characterization of novel inorganic/organometallic compounds
- Genomics, phytochemicals, and oxidative stress
- Metal complexes synthesis and properties
- Cancer, Hypoxia, and Metabolism
University of Tsukuba
2015-2025
Tohoku University
1955-2025
Aso Iizuka Hospital
2014-2025
Precision Research (United States)
2025
University of Tsukuba Hospital
2013-2024
Kyushu University
2016-2024
Suzuki (Japan)
2014-2024
Weatherford College
2024
University Gastroenterology
2019-2024
Keiyu Orthopedic Hospital
2022-2023
Tumor necrosis factor alpha (TNF-α) is a macrophage/monocyte-derived polypeptide which modulates the expression of various genes in vascular endothelial cells and induces angiogenesis. However, underlying mechanism by TNF-α mediates angiogenesis not completely understood. In this study, we assessed whether TNF-α-induced mediated through itself or indirectly other angiogenesis-promoting factors. Cellular mRNA levels interleukin-8 (IL-8), growth (VEGF), basic fibroblast (bFGF), their receptors...
Background: The clinical outcome is generally positive for patients with node-negative breast carcinoma (i.e., those who do not have detectable metastases in the lymph nodes) been treated surgery or plus radiation therapy. In about 30% of patients, however, disease recurs, and they are at risk death. Determination valid new prognostic indicators would improve ability to identify high recurrence. Breast cancer can entail substantial development blood vessels within tumor tissue, it known that...
Abstract The isothiocyanate sulforaphane [SF; 1-isothiocyanato-4(R)-methylsulfinylbutane] is abundant in broccoli sprouts the form of its glucosinolate precursor (glucoraphanin). SF powerfully bactericidal against Helicobacter pylori infections, which are strongly associated with worldwide pandemic gastric cancer. Oral treatment SF-rich C57BL/6 female mice infected H. Sydney strain 1 and maintained on a high-salt (7.5% NaCl) diet reduced bacterial colonization, attenuated mucosal expression...
Recent clinical studies have demonstrated that tumor angiogenesis is a potent prognostic indicator for breast cancer patients. The quantitation of endothelial growth factors thought to be useful assess angiogenic phenotype in the tumor. Among many new factors, vascular factor (VEGF) known particularly responsible promoting neovascularization human cancer.Intratumoral protein levels VEGF were measured by enzymatic immunoassay 135 primary tissues. compared with microvessel density evaluated...
Angiotensin converting enzyme (ACE) is a peptidyldipeptide hydrolase (EC 3.4.15. 1) which cleaves dipeptides from the free COOH-terminal of peptides.The hydrolyzes both angiotensin I and bradykinin, generating potent vasoconstrictor II I, vasoinactive peptides vasodilator bradykinin.Inhibitors ACEwere first obtained
Inhibitors of angiotensin I-converting enzyme were isolated from an enzymatic hydrolysate bovine casein. The amino acid sequences these inhibitors Phe-Phe-Val-Ala-Pro-Phe-Pro- Glu-Val-Phe-Gly-Lys (CEI12), Phe-Phe-Val-Ala-Pro (CEI5), and Ala-Val-Pro-Tyr-Pro-Gln-Arg (CEIβ7). CEI5 is a penta-peptide derived the CEI12 with proline-specific endopeptidase, CEIβ7 hepta-peptide β-casein. These potentiated bradykinin in contraction uterus ileum rats. was more sensitive to than uterus....
A simple method has been devised for the isolation of a-galactosidase in crystalline form from mycelia Mortierella vinacea.The cr-galactosidase is free protease and other glycosidases.It gives a single protein band when examined by polyacrylamide gel electrophoresis.The preparation contains 10.8% neutral sugars 2.7% n-glucosamine, indicating that enzyme glycoprotein nature.The stable at alkaline pH; however, it becomes unstable below pH 6.0.It hydrolyzes 0-and...
We investigated the role of cMOAT/MRP2 (canalicular multispecific organic anion transporter/multidrug resistance-associated protein 2) in intestinal secretion anions by comparing behavior Sprague-Dawley (SD) rats and Eisai hyperbilirubinemic rat (EHBR) whose is hereditarily defective. After i.v. administration 1-chloro-2,4-dinitrobenzene (30 micromol/kg), biliary excretion its glutathione conjugate 2, 4-dinitrophenyl-S-glutathione (DNP-SG), a substrate for cMOAT/MRP2, was significantly...
The fragment-peptides of angiotensin I-converting enzyme inhibitors (CEI12, CEI5 and CEIβ7) derived from an enzymatic hydrolysate casein were synthesized. Val-Ala-Pro, the C-terminal tripeptide (Phe-Phe-Val-Ala-Pro), exhibited more potent inhibitory activity (I50=2.0μM) than (I50=6.0μM). However, D-Val-Ala-Pro showed lower (I50=550μM). Val-Ala-Pro may be important for CEI5. heptapeptide CEI12 (Phe-Phe-Val-Ala-Pro-Phe-Pro-Glu-Val-Phe-Gly-Lys) short fragments CEIβ7...
Thr-Thr-Met-Pro-Leu-Trp, the C-terminal hexapeptide of αsl-casein, inhibited angiotensin I-converting enzyme (I50 = 16 μM). Z-Pro-Leu-Trp also 18 The Pro- Leu-Trp sequence may be important for inhibitory activity this hexapeptide. Z-Pro-Val-Trp, which contains Val in place Leu, more potently =2.9 antihypertensive was investigated. This peptide, when intravenously administered to anesthetized rats at 31.8 mg/kg, tended antagonize rats' pressor response I.
Inhibitors of angiotensin I-converting enzyme were isolated from an enzymatic hydrolysate bovine casein. The amino acid sequences these inhibitors Phe-Phe-Val-Ala-Pro-Phe-Pro-Glu-Val-Phe-Gly-Lys (CEI12), Phe-Phe-Val-Ala-Pro (CEI5), and Ala-Val-Pro-Tyr-Pro-Gln-Arg (CEIβ7). CEI5 is a penta-peptide derived the CEI12 with proline-specific endopeptidase, CEIβ7 hepta-peptide β-casein. These potentiated bradykinin in contraction uterus ileum rats. was more sensitive to than uterus....
Ulcerative colitis (UC) is a chronic inflammatory bowel disease that may become intractable when treated with conventional medications such as aminosalicylates, corticosteroids, and azathioprine. The herbal medicine Qing Dai has traditionally been used in Chinese to treat UC patients, but there lack of published data on the efficacy treatment. We report several cases patients who take retrospective observational study. Furthermore, we explore mechanisms action Dai. Nine active received...
Aim . Immune checkpoint inhibitors (ICIs) have improved the survival rate of patients carrying various malignant neoplasms. Despite their efficacy, ICIs occasionally induce liver injury as an immune-related adverse event (irAE). This study aimed to reveal clinical features hepatic irAE in Japanese patients. Methods Among 387 treated with ICIs, those who developed drug-induced were investigated. We also describe histological findings and courses four underwent biopsy. Results 56 all-grade...