A5066861560- Metalloenzymes and iron-sulfur proteins
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Adaptive optics and wavefront sensing
- Optical measurement and interference techniques
- Vibrio bacteria research studies
- Listeria monocytogenes in Food Safety
- Amino Acid Enzymes and Metabolism
- Tuberculosis Research and Epidemiology
- Mitochondrial Function and Pathology
- Identification and Quantification in Food
- Redox biology and oxidative stress
- Cancer, Hypoxia, and Metabolism
- Image Processing Techniques and Applications
- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
- Trace Elements in Health
- Metal-Organic Frameworks: Synthesis and Applications
- Optical Systems and Laser Technology
- Bacterial Genetics and Biotechnology
- Endoplasmic Reticulum Stress and Disease
- ATP Synthase and ATPases Research
- Pneumocystis jirovecii pneumonia detection and treatment
Cornell University
2017-2023
Wake Forest University
2014-2022
Weill Cornell Medicine
2019-2021
Boeing (United States)
2002-2003
Significance Tuberculosis (TB) remains a global health concern despite the availability of antibiotics to treat this disease. Understanding metabolic state bacteria during infection is crucial identifying novel drug targets that are vital Mycobacterium tuberculosis survival. Here, we demonstrate M. central carbon metabolism altered in response acidic pH, host-imposed stress, causing pathogen depend on lipids for growth. Continuous fatty acid supplementation at pH simple and effective...
Bacterial pathogens must adapt to their host environment in order cause disease. The cytosolic bacterial pathogen Listeria monocytogenes requires a highly conserved protein of unknown function, GlmR (previously known as YvcK), survive the cytosol.
The 2-thiouridine (s(2)U) modification of the wobble position in glutamate, glutamine, and lysine tRNA molecules serves to stabilize anticodon structure, improving ribosomal binding overall efficiency translational process. Biosynthesis s(2)U Escherichia coli requires a cysteine desulfurase (IscS), thiouridylase (MnmA), five intermediate sulfur-relay enzymes (TusABCDE). E. MnmA adenylates subsequently thiolates form modification. Bacillus subtilis lacks IscS sulfur relay proteins, yet its...
The biosynthesis of Fe–S clusters and other thio-cofactors requires the participation redox agents. A shared feature in these pathways is formation transient protein persulfides, which are susceptible to reduction by artificial reducing agents commonly used reactions vitro. These modulate reactivity catalytic efficiency biosynthetic and, some cases, skew enzymes' kinetic behavior, bypassing sulfur acceptors known be critical for functionality vivo. Here, we provide evidence selective...
Posttranscriptional modifications to tRNA are critical elements for the folding and functionality of these adaptor molecules. Sulfur in installed by specialized enzymes that act on cognate substrates at specific locations. Most studied organisms contain a general cysteine desulfurase mobilize sulfur synthesis S-tRNA other thio-cofactors. Bacillus subtilis Gram-positive bacteria encode multiple desulfurases partner with acceptors biosynthesis This metabolic layout suggests an alternate mode...
Phosphohexomutase superfamily enzymes catalyze the reversible intramolecular transfer of a phosphoryl moiety on hexose sugars. Bacillus subtilis phosphoglucomutase PgcA catalyzes interconversion glucose 6-phosphate (Glc-6-P) and 1-phosphate (Glc-1-P), precursor UDP-glucose (UDP-Glc). B. phosphoglucosamine mutase (GlmM) is member same enzyme that converts glucosamine (GlcN-6-P) to (GlcN-1-P), amino sugar peptidoglycan. Here, we present evidence possesses activity as contributes peptidoglycan...
We present the results of phase-diversity algorithm applied to simulated and laboratory data. show that exact amount defocus distance does not need be known exactly for on extended scene imaging. determine, through computer simulation, optimum diversity various types. Using data, we compare aberrations recovered with those measured a Fizeau interferometer uses He-Ne laser. The two aberration sets agree Strehl ratio over 0.9. contrast object is found ten times raw image.
Abstract The cytosol of eukaryotic host cells is an intrinsically hostile environment for bacteria. Understanding how cytosolic pathogens adapt to and survive in the critical developing novel therapeutic interventions these pathogens. pathogen Listeria monocytogenes requires glmR (previously known as yvcK ), a gene unknown function, resistance cell wall stress, survival, inflammasome avoidance ultimately virulence vivo . A genetic suppressor screen revealed that blocking utilization...
Comparative sequence analysis has enabled the annotation of millions genes from organisms across evolutionary tree. However, this approach inherently biased phylogenetically ubiquitous, rather than species-specific, functions. The ecologically unusual pathogen Mycobacterium tuberculosis (Mtb) evolved in humans as its sole reservoir and emerged leading bacterial cause death worldwide. physiological factors that define Mtb’s pathogenicity are poorly understood. Here, we report structure...
Abstract Sulfur incorporation into the molybdenum cofactor (Moco) in Gram-negative bacterium Escherichia coli involves six enzymes. The initial reaction includes cysteine desulfurase IscS, sulfurtransferase TusA, and rhodanese domaincontaining protein YnjE. Gram-positive Bacillus subtilis contains no direct homologs for but rather four distinct desulfurases (YrvO, NifS, NifZ, SufS) YrkF, a two-domain with an N-terminal domain similar to TusA. Bioinformatic analysis was used identify...
Post‐transcriptional modifications of tRNA are found in all organisms and critical for their structure function during translation. 2‐thiouridine (s 2 U) modification at the wobble position Glu, Gln, Lys is required accurate efficient protein synthesis its absence impacts cellular viability. In addition to role translation, yeast, s U used as a sensor gauge nutrient availability regulate translational capacity amino acid homeostasis, which abundance reflects sulfur‐containing acids, cysteine...
In this paper, we present the results of phase diversity algorithm applied to simulated and laboratory data. We show that exact amount defocus distance does not need be known exactly for algorithm. determine, through simulation, optimum distance. compare aberrations recovered with those measured a Fizeau interferometer using HeNe laser. The two aberration sets agree Strehl over 0.9. contrast object is found 10 times raw image.