A5045561300- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Uterine Myomas and Treatments
- Nanowire Synthesis and Applications
- CAR-T cell therapy research
- Cancer-related molecular mechanisms research
- Reproductive Biology and Fertility
- Virus-based gene therapy research
- Integrated Circuits and Semiconductor Failure Analysis
- Cancer, Hypoxia, and Metabolism
- Food Security and Health in Diverse Populations
- Circular RNAs in diseases
- Cancer Mechanisms and Therapy
- Prenatal Screening and Diagnostics
- Folate and B Vitamins Research
- Cancer-related gene regulation
- Acute Lymphoblastic Leukemia research
- Pregnancy and Medication Impact
- Endometrial and Cervical Cancer Treatments
- Cancer Risks and Factors
- Extracellular vesicles in disease
- Ovarian function and disorders
- Ferroptosis and cancer prognosis
Second Xiangya Hospital of Central South University
2013-2025
Central South University
2013-2025
Case Western Reserve University
2012
Jinan University
2012
Mayo Clinic
2012
Abstract IL6 is one of the most elevated cytokines during chimeric antigen receptor (CAR) T cell cytokine release syndrome (CRS), and IL6R blockade by Tocilizumab has successfully relieved life-threatening aspects CRS in patients. In addition, latest studies demonstrated essential role IL1 driving CART induced neurotoxicity mouse models. Here we present a clinical investigation (ChiCTR2000032124; ChiCTR2000031868) anti-CD19 anti-BCMA (41BBζ) secreting an anti-IL6 scFv antagonist (IL1RA)...
Abstract Revolutionary CART therapy still faces the challenge of severe cytokine release syndrome (CRS). While IL6 and IL1 have been demonstrated as essential contributors, GM-CSF is one most abundant inflammatory cytokines secreted by has also suggested in contributing to CRS. To minimize production from reduce its associated toxicity, we conducted a pilot study (ChiCTR2000032124) CRISPR-edited knockout (KO) secreting anti-IL6 scFv IL1RA, with additional TCR KO for tracing edited CART. The...
Cervical cancer (CC) is a prevalent in women. Hypoxia plays critical role CC cell ferroptosis resistance. This study explored the mechanism of hypoxia resistance by regulating HIF1α/KDM4A/H3K9me3. Cultured SiHa and Hela cells were exposed to CoCl2 treated with Erastin. Cell viability was detected MTT assay, concentrations iron ion, MDA GSH determined using corresponding kits. Expressions KDM4A, HIF1α, TfR1, DMT1, H3k9me3 RT-qPCR, Western blot, ChIP assay. The correlation KDM4A HIF1α analyzed...
Abstract Background This study explored the key genes related to immune cell infiltration in endometriosis. Results The Gene Expression Omnibus (GEO) datasets (GSE7305, GSE7307, and GSE11691), containing a total of 37 endometriosis 42 normal tissues, were retrieved analyzed determine differentially expressed (DEGs). ontology (GO) annotations Kyoto Encyclopedia Genes (KEGG) analysis performed identify pathways that significantly enriched. xCell software was used analyze correlation analyses...
The discovery of ferroptosis has unveiled new perspectives for cervical cancer (CC) management. We elucidated the functional mechanism hypoxia-like conditions in CC cell resistance. cells were subjected to normoxia or conditions, followed by erastin treatment induce ferroptosis. assessment viability/ferroptosis resistance was performed MTT assay/Fe
Endometriosis is a chronic inflammatory disease with an uncertain pathogenesis. Peritoneal immune dysregulation plays important role in the pathogenesis of endometriosis. Exosomes are messengers intercellular communication. This study mainly investigated exosomes from uterine cavity aspirate fluid were isolated and cocultured macrophages for 48 h. Flow cytometry was used to detect macrophage polarization. A Human MAPK Phosphorylation Antibody Array Western blot phosphorylation pathway....
Abstract Lactate is a major metabolic product of tumor cells in microenvironment. Increasing evidence has indicated that lactate accumulation could alter the immune response human cancers, including cervical cancer. However, function and significance N 6 -methyladenosine (m A) reader YTHDF1 cancer cells’ metabolism immunotherapy remain obscure. Results illustrated predicted unfavorable clinical outcomes cancer, which was negatively correlated with CD8 + T cell infiltration. In co-culture...
The "methionine cycle" plays a critical role in the regulation of concentrations (<i>S</i>)-adenosylmethionine (AdoMet), major biological methyl donor. We set out to study sequence variation genes encoding enzyme that synthesizes AdoMet liver, methionine adenosyltransferase 1A (MAT1A) and hepatic using enzyme, glycine <i>N</i>-methyltransferase (GNMT), as well functional implications variation. resequenced <i>MAT1A</i> <i>GNMT</i> DNA from 288 subjects three ethnicities, followed by genomic...
Macrophage‑induced inflammation is a major factor in the pathogenesis of endometriosis. The underlying mechanisms, however, remain largely unknown. TNF‑α, IL‑6, IL‑10 and C‑C motif chemokine 20 (CCL20) levels endometrial extracts were determined using Luminex cytokine kits. Additionally, protein arginine methyltransferase 5 (PRMT5) measured reverse transcription‑quantitative PCR western blotting. IL‑6 IP‑10 cells ELISA In present study, it was revealed that PRMT5 expression at both mRNA...
Abstract Background Endometriosis development is associated with peritoneal immune microenvironment abnormality. However, the specific mechanism uncertain. Investigating regulation mechanisms could introduce novel therapeutic strategies for effective endometriosis treatment. Methods Wound healing assay, Transwell, colony formation were carried out to assess cells invasion and proliferation. Western blotting analysis ELISA used evaluate resistance progesterone. C57BL6 mouse model was...