A5041465867- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Advanced Electron Microscopy Techniques and Applications
- Genomics and Chromatin Dynamics
- Nanocluster Synthesis and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Lipid Membrane Structure and Behavior
- RNA modifications and cancer
- Force Microscopy Techniques and Applications
- Nanomaterials for catalytic reactions
- Monoclonal and Polyclonal Antibodies Research
- Wnt/β-catenin signaling in development and cancer
- Fungal and yeast genetics research
- Water Quality Monitoring and Analysis
- Pesticide Residue Analysis and Safety
- Graphene and Nanomaterials Applications
- Advanced Fluorescence Microscopy Techniques
- Spectroscopy and Chemometric Analyses
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Fibroblast Growth Factor Research
- Connective tissue disorders research
Stanford University
2008-2025
Stanford Medicine
2012-2024
Hebrew University of Jerusalem
2004-2005
Fundación Ciencias Exactas y Naturales
1999
Structure determination of gold nanoparticles (AuNPs) is necessary for understanding their physical and chemical properties, but only one AuNP larger than 1 nanometer in diameter [a 102-gold atom NP (Au102NP)] has been solved to atomic resolution. Whereas the Au102NP structure was determined by x-ray crystallography, other large AuNPs have proved refractory this approach. Here, we report a Au68NP at resolution aberration-corrected transmission electron microscopy, performed with use minimal...
The protein density and arrangement of subunits a complete, 32-protein, RNA polymerase II (pol II) transcription pre-initiation complex (PIC) were determined by means cryogenic electron microscopy combination chemical cross-linking mass spectrometry. PIC showed marked division in two parts, one containing all the general factors (GTFs) other pol II. Promoter DNA was associated only with GTFs, suspended above cleft not contact This structural principle underlies its conversion to...
By a modification of the method Brust et al., water-soluble, thiolate-protected gold nanoparticles that are uniform in size were synthesized with no requirement for purification. The was equilibration first step, which proved crucial achieving homogeneity. thiol-to-gold ratio controlled particles, and choice thiol reactivity particles toward exchange.
Structure determination by transmission electron microscopy has revealed the long sought 144-gold atom particle. The structure exhibits deviations from face-centered cubic packing of gold atoms, similar to solution another nanoparticle, and in contrast a previous X-ray crystal structure. Evidence analytical methods points low number 3-mercaptobenzoic acid ligands covering surface
Zinc fingers (ZFs) are compact, modular, sequence-specific polynucleotide-binding domains uniquely suited for use as DNA probes and the targeted delivery of effector purposes such gene regulation editing. Despite recent advances in both design application ZF-containing proteins, there is still a lack general method their expression purification. Here we describe simple method, involving two chromatographic steps, production homogeneous, functional ZF proteins high yield (one milligram per...
Structures of complete 10-subunit yeast TFIIH and a nested set subcomplexes, containing 5, 6, 7 subunits, have been determined by electron microscopy (EM) 3D reconstruction. Consistency among all the structures establishes location "minimal core" subunits (Ssl1, Tfb1, Tfb2, Tfb4, Tfb5), additional densities can be specifically attributed to Rad3, Ssl2, TFIIK trimer. These results further interpreted placement previous X-ray into give preliminary picture RNA polymerase II preinitiation...
The fibroblast growth factor FGF21 was labeled with molecularly defined gold nanoparticles (AuNPs), applied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET). Most AuNPs were in pairs about 80 Å apart, on the outer cell surface. Pairs of also abundant inside cells clathrin-coated vesicles endosomes. present but no longer paired multivesicular bodies. could thus be tracked along endocytotic pathway. methods developed here visualize signaling coupled endocytosis can a wide...
Most synapses in the brain transmit information by presynaptic release of vesicular glutamate, driving postsynaptic depolarization through AMPA-type glutamate receptors (AMPARs). The nanometer-scale topography synaptic AMPARs regulates response amplitude controlling number activated vesicle fusion. mechanisms AMPAR and their interactions with scaffolding proteins are unclear, as is spatial relationship between vesicles. Here, we used cryo-electron tomography to map molecular visualize