A5030632893- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
Weizmann Institute of Science
2025
Technion – Israel Institute of Technology
2019-2021
Analysis of single-molecule fluorescent in situ hybridization (smFISH) images is important to translate cellular image data into a quantifiable format. Although smFISH the gold standard for RNA localization measurements, there are no freely available, user-friendly applications assaying messenger (mRNA) organelles. EASI-ORC (Efficient and Segmentation Images Organelle-RNA Colocalization) novel pipeline automated analysis multiple yeast cells. automates segmentation cells organelles,...
Aminoacyl-tRNA synthetases (aaRSs) are a conserved family of enzymes with an essential role in protein synthesis: ligating amino acids to cognate tRNA molecules for translation. In addition their charging, aaRSs have acquired non-canonical functions, including post-transcriptional regulation mRNA expression. Yet, the extent and mechanisms these functions largely unknown. Herein, we performed comprehensive transcriptome analysis define mRNAs that associated almost all present
Analysis of single-molecule fluorescent in situ hybridization (smFISH) images is an arduous and time-consuming task that important to perform accurately order translate image data into a quantifiable format. This becomes increasingly more difficult greater the experimental scope number captured. Although smFISH gold standard for RNA localization measurements, there are no freely available, user-friendly applications assaying messenger sub-cellular structures, like endoplasmic reticulum (ER)...