A5025535554- Wastewater Treatment and Nitrogen Removal
- Ammonia Synthesis and Nitrogen Reduction
- Microbial Community Ecology and Physiology
- Water Treatment and Disinfection
- Fecal contamination and water quality
- Constructed Wetlands for Wastewater Treatment
- Urban Stormwater Management Solutions
- Legionella and Acanthamoeba research
- Water Systems and Optimization
- Climate Change and Health Impacts
- Tracheal and airway disorders
- Health, Environment, Cognitive Aging
- Air Quality and Health Impacts
- Per- and polyfluoroalkyl substances research
- Radioactivity and Radon Measurements
- Gut microbiota and health
University of Washington
2024
Northeastern University
2019-2024
Georgia Institute of Technology
2022-2024
Cooperation between comammox and anammox bacteria for nitrogen removal has been recently reported in laboratory-scale systems, including synthetic community constructs; however, there are no reports of full-scale municipal wastewater treatment systems with such cooperation. Here, we report intrinsic extant kinetics as well genome-resolved characterization a integrated fixed film activated sludge (IFAS) system where co-occur appear to drive loss. Intrinsic batch kinetic assays indicated that...
This study performed a comprehensive assessment of the impact Hurricane Maria (HM) on drinking water quality in Puerto Rico (PR) by integrating targeted chemical analysis both inorganic (18 trace elements) and organic pollutants (200 micropollutants) with high-throughput quantitative toxicogenomics vitro biomarkers-based toxicity assays. Average concentrations 14 detected elements 20 micropollutants showed elevation after HM. Arsenic, sucralose, perfluorooctanoic acid (PFOA),...
COVID-19 pandemic-related building restrictions heightened drinking water microbiological safety concerns post-reopening due to the unprecedented nature of commercial closures. Starting with phased reopening (i.e., June 2020), we sampled for 6 months from three buildings reduced usage and four occupied residential households. Samples were analyzed using flow cytometry full-length 16S rRNA gene sequencing along comprehensive chemistry characterization. Prolonged closures resulted in 10-fold...
While the co-existence of comammox
Leveraging comammox
Complete ammonia oxidizing bacteria coexist with canonical and nitrite in a wide range of environments. Whether this is due to competitive or cooperative interactions, result niche separation not yet clear. Understanding the factors driving coexistence nitrifiers critical manage nitrification processes occurring engineered natural ecosystems. In study, microcosm-based experiments were used investigate impact nitrogen source loading on population dynamics drinking water biofilter media....
Loss of basic utilities, such as drinking water and electricity distribution, were sustained for months in the aftermath Hurricane Maria's (HM) landfall Puerto Rico (PR) September 2017. The goal this study was to assess if there deterioration biological quality due these disruptions. This characterized microbial composition following HM across nine systems (DWSs) PR utilized an extended temporal sampling campaign determine changes microbiome indicative associated disturbance followed by...
Leveraging comammox Nitrospira and anammox bacteria for shortcut nitrogen removal can drastically lower the carbon footprint of wastewater treatment facilities by decreasing aeration energy, carbon, alkalinity, tank volume requirements while also potentially reducing nitrous oxide emissions. However, their co-occurrence as dominant nitrifying is rarely reported in full-scale treatment. As a result, there poor understanding how operational parameters, particular dissolved oxygen, impact...
Abstract Building closures related to the coronavirus disease (COVID-19) pandemic resulted in increased water stagnation commercial building plumbing systems that heightened concerns microbiological safety of drinking post re-opening. The exact impact extended periods reduced demand on quality is currently unknown due unprecedented nature widespread closures. We analyzed 420 tap samples over a period six months, starting month phased reopening (i.e., June 2020), from sites at three buildings...
ABSTRACT The discovery of the complete ammonia oxidizing (comammox) bacteria overturns traditional two-organism nitrification paradigm which largely underpins design and operation nitrogen removal during wastewater treatment. Quantifying abundance, diversity, activity comammox in treatment systems is important for ensuring a clear understanding biotransformations responsible removal. To this end, we conducted yearlong survey 14 full-scale including mainstream conventional simultaneous...
Abstract Studies have found Ca . Nitrospira nitrosa-like bacteria to be the principal or sole comammox in nitrogen removal systems for wastewater treatment. In contrast, multiple populations of strict ammonia and nitrite oxidizers co-exist similar systems. This apparent lack diversity is surprising could impact feasibility leveraging removal. We used full-length 16S rRNA gene sequencing genome-resolved metagenomics compare population-level (i.e., species) with that nitrifiers full-scale...
Abstract Loss of basic utilities, such as drinking water and electricity distribution, were sustained for months in the aftermath Hurricane Maria’s (HM) landfall Puerto Rico (PR) September 2017. The goal this study was to assess if there deterioration biological quality due these disruptions. This characterized microbial composition following HM across nine systems (DWSs) PR utilized an extended temporal sampling campaign determine changes microbiome indicative associated disturbance...
Abstract While the co-existence of comammox bacteria with canonical nitrifiers is well documented in diverse ecosystems, there still a dearth knowledge about mechanisms underpinning their interactions. Understanding these interaction important as they may play critical role governing nitrogen biotransformation natural and engineered ecosystems. In this study, we tested ability two environmentally relevant factors (nitrogen source availability) to shape interactions between strict ammonia...
ABSTRACT Cooperation between comammox and anammox bacteria for nitrogen removal has been recently reported in laboratory-scale systems including synthetic community construct; however, there are no reports of full-scale municipal wastewater treatment with such cooperation. Here, we report intrinsic extant kinetics as well genome-resolved characterization a integrated fixed film activated sludge (IFAS) system where co-occur appear to drive loss. Intrinsic batch kinetic assays indicated that...
Abstract Complete ammonia oxidizing bacteria coexist with canonical and nitrite in a wide range of environments. Whether this is due to competitive or cooperative interactions, result niche separation not yet clear. Understanding the factors driving coexistence nitrifiers critical manage nitrification processes occurring engineered natural ecosystems. In study, microcosm-based experiments were used investigate impact nitrogen source loading on population dynamics drinking water biofilter...