A5037825119- NMR spectroscopy and applications
- Advanced MRI Techniques and Applications
- Advanced NMR Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Microfluidic and Capillary Electrophoresis Applications
- Electrowetting and Microfluidic Technologies
- Various Chemistry Research Topics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Analytical Chemistry and Chromatography
- Experimental Learning in Engineering
- Molecular spectroscopy and chirality
- Isotope Analysis in Ecology
- Electrochemical Analysis and Applications
- Physiological and biochemical adaptations
- Marine Biology and Ecology Research
- Marine and coastal ecosystems
- Mobile Learning in Education
- Analytical Chemistry and Sensors
- Atmospheric and Environmental Gas Dynamics
- Aquaculture disease management and microbiota
- Innovative Teaching and Learning Methods
- Innovative Teaching Methods
- Educational Strategies and Epistemologies
- Water Quality Monitoring and Analysis
- Water Quality Monitoring Technologies
The Scarborough Hospital
2018-2024
University of Toronto
2018-2024
Toronto Public Health
2018-2021
In U.S. Pacific Northwest coho salmon (
We describe a two-plate digital microfluidic method for interfacing with nuclear magnetic resonance spectroscopy (DMF-NMR) microscale chemical analysis.
Abstract NMR/MRI are critical tools for studying molecular structure and interactions but suffer from relatively low sensitivity spectral overlap. Here, a Nuclear Magnetic Resonance (NMR) approach, termed DREAMTIME, is introduced that provides “a window” inside complex systems, capable of showing only what the user desires, with complete specificity. The chooses list molecules interest, approach detects those targets while all other invisible. demonstrated in whole human blood urine, small...
Microcoil nuclear magnetic resonance (NMR) has been interfaced with digital microfluidics (DMF) and is applied to monitor organic reactions in solvents as a proof of concept. DMF permits droplets be moved mixed inside the NMR spectrometer initiate while using sub-microliter volumes reagent, opening up potential follow scarce or expensive reagents. By setting shims on reagent droplet, data acquisition can started immediately upon droplet mixing only limited by rate at which collected,...
Abstract Synergism between different phases gives rise to chemical, biological or environmental reactivity, thus it is increasingly important study samples intact. Here, SASSY (SimultAneous Solid and Solution spectroscopY) introduced simultaneously observe (and differentiate) all in multiphase using standard, solid‐state NMR equipment. When monitoring processes, the traditional approach of studying solids liquids sequentially, can lead information non‐observed phase being missed. solves this...
Chemical characterization of complex mixtures by Nuclear Magnetic Resonance (NMR) spectroscopy is challenging due to a high degree spectral overlap and inherently low sensitivity. Therefore, NMR experiments that reduce increase signal intensity hold immense potential for the analysis such as biological environmental media. Here, we introduce 13C version DREAMTIME (Designed Refocused Excitation And Mixing Targets In Vivo Mixture Elucidation) NMR, which, when analyzing 13C-enriched materials,...
In recent years there has been a renewed interest in benchtop NMR. Given their lower cost of ownership, smaller footprint, and ease use, they are especially suited as an educational tool. Here, new experiment targeted at upper-year undergraduates first-year graduate students follows the conversion D-glucose into ethanol low-field. First, high low-field data on compared learn both Hz ppm scales how J-coupling is field-independent. The then acquire own quantitative NMR datasets perform...
Due to the COVID-19 pandemic, social distancing restrictions are in place most public settings, and undergraduate laboratory is no exception. In order accommodate requirements, many exercises being redeployed an online format, which deprives students of experiential learning opportunities a real setting. To bridge this gap for exercises, open-source remote titration unit was created. This based on simple Raspberry Pi architecture equipped with webcam servo (a small motor allowing fine...
Toxicity testing is currently undergoing a paradigm shift from examining apical end points such as death, to monitoring sub-lethal toxicity in vivo. In vivo nuclear magnetic resonance (NMR) spectroscopy key platform this endeavor. A proof-of-principle study presented which directly interfaces NMR with digital microfluidics (DMF). DMF "lab on chip" method allowing for the movement, mixing, splitting, and dispensing of μL-sized droplets. The goal supply oxygenated water keep organisms alive...
Titration is a common introductory experiment performed across teaching laboratories from high school to university. Yet, its setup remains inaccessible for students with disabilities, denying them the opportunity experiential learning. Therefore, rethinking such required increase laboratory participation these students. To remove physical barriers, an automated titration unit based on existing undergraduate setups and universal design concepts, coupled text-to-speech (TTS) capability,...
Nuclear Magnetic Resonance (NMR) spectroscopy is a non-invasive analytical technique which allows for the study of intact samples. Comprehensive Multiphase NMR (CMP-NMR) combines techniques and hardware from solution state solid to allow holistic analysis all phases (i.e. solutions, gels solids) in unaltered This first apply CMP-NMR deceased, organisms uses 13C enriched Daphnia magna (water fleas) as an example. D. are commonly used model environmental toxicology studies. As primary...
Structure determination via NMR spectroscopy is a fundamental topic taught in many advanced level chemistry courses. Despite its importance as an analytical instrument, students find the principle behind NMR-facilitated structure difficult to embrace. To this end, artificial intelligence (AI) software package, namely, ACD/Structure Elucidator, used facilitate teaching of spectroscopic principles and application de novo elucidation large complex organic molecules upper class. For purpose...
Nuclear magnetic resonance (NMR) spectroscopy has played an integral role in medical and environmental metabolic research. However, smaller biological entities, such as eggs small tissue samples, are becoming increasingly important to better understand toxicity, growth/development, diseases. Unfortunately, their sizes make them difficult study using conventional 5 mm NMR probes due limited sensitivity. The use of microcoil holds great potential for the analysis where coil can be designed...
Comprehensive multiphase–nuclear magnetic resonance (CMP–NMR) is a non-invasive approach designed to observe all phases (solutions, gels, and solids) in intact samples using single NMR probe. Studies of dead living organisms are important understand processes ranging from biological growth environmental stress. Historically, such studies have utilized 1H-based phase editing for the detection soluble/swollen components 1H-detected 2D metabolite assignments/screening. However, require slow...
With the advances in open-source technology, many laboratory devices can be built with a modest budget, particularly assistive devices. The current market cost of most is major barrier for students accessibility needs. Specifically, visual impairment, their limited choice has motivated us to construct low-cost, multipurpose sensor hub capable connecting pH electrode or thermocouple, two sensors commonly used chemistry teaching laboratories. In this technology report, we present construction...
ABSTRACT Self-regulated learning (SRL) is the process of utilizing effective strategies to acquire knowledge or skills and influenced by motivation, metacognitive processing, study-related behaviors. We hypothesized that using survey tools allow reflection on refinement students’ study strategies, we could nurture skill development, encourage positive motivation behaviors, hence promote academic success. Undergraduate students in a semester-long, second-year biology course were provided with...
The ability to visualize chemical processes can be an asset in a teaching laboratory. Despite the advances laboratory technology, apparatuses used of simple thermodynamics concepts, such as exothermic/endothermic reactions, historically remain unchanged and benefit from fresh perspective. recent incorporation thermal imaging cameras into chemistry has enabled students better grasp concepts thermodynamics. In fact, most visual learners will this approach, advocating its importance...
Current research is attempting to address more complex questions than ever before. As such, the need follow processes in intact media and mixtures becoming commonplace. Here, a targeted NMR experiment introduced which selectively detects formation of 13C-12C bonds mixtures. This study introduces on simple standards, then demonstrates potential increasingly including: fermentation, Arabidopsis thaliana germination/early growth, metabolism Daphnia magna both ex vivo vivo. signals from 12C 13C...
Abstract NMR/MRI are critical tools for studying molecular structure and interactions but suffer from relatively low sensitivity spectral overlap. Here, a Nuclear Magnetic Resonance (NMR) approach, termed DREAMTIME, is introduced that provides “a window” inside complex systems, capable of showing only what the user desires, with complete specificity. The chooses list molecules interest, approach detects those targets while all other invisible. demonstrated in whole human blood urine, small...
Environmental metabolomics provides insight into how anthropogenic activities have an impact on the health of organism at molecular level. Within this field, in vivo NMR stands out as a powerful tool for monitoring real-time changes organism's metabolome. Typically, these studies use 2D 13C-1H experiments 13C-enriched organisms. Daphnia are most studied species, given their widespread toxicity testing. However, with COVID-19 and other geopolitical factors, cost isotope enrichment increased...
Comprehensive multiphase NMR combines the ability to study and differentiate all phases (solids, gels, liquids) using a single probe. The general goal of CMP-NMR is intact environmental biological samples better understand conformation, organization, association, transfer between across phases/interfaces that may be lost with conventional sample preparation such as drying or solubilization. To date, studies have used 4 mm probes rotors. Here, larger 7 probehead introduced which provides ∼3...