A5074818775- Bacillus and Francisella bacterial research
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Immune cells in cancer
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- Escherichia coli research studies
- Cancer Research and Treatments
- Viral gastroenteritis research and epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Brucella: diagnosis, epidemiology, treatment
- Congenital Diaphragmatic Hernia Studies
- Lipid Membrane Structure and Behavior
- Cystic Fibrosis Research Advances
- Caveolin-1 and cellular processes
- Medical Imaging and Pathology Studies
- Neuroendocrine Tumor Research Advances
- Ferroptosis and cancer prognosis
- Infections and bacterial resistance
- Peptidase Inhibition and Analysis
- 3D Printing in Biomedical Research
- Hepatitis B Virus Studies
- Salmonella and Campylobacter epidemiology
- Pneumonia and Respiratory Infections
- Bacterial biofilms and quorum sensing
Washington University in St. Louis
2020-2025
Jewish Hospital
2022
Barnes-Jewish Hospital
2022
Jawaharlal Nehru University
2015-2020
Milton Keynes Hospital
2013-2014
Milton Keynes Hospital NHS Foundation Trust
2013-2014
Abstract Targeting the mitogen-activated protein kinase (MAPK) cascade in pancreatic ductal adenocarcinoma (PDAC) remains clinically unsuccessful. We aim to develop a MAPK inhibitor-based therapeutic combination with strong preclinical efficacy. Utilizing reverse-phase array, we observe rapid phospho-activation of human epidermal growth factor receptor 2 (HER2) PDAC cells upon pharmacological inhibition. Mechanistically, inhibitors lead swift proteasomal degradation dual-specificity...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers with limited effectiveness to immune checkpoint therapies, primarily due its immunological “cold” tumor microenvironment (TME). We identified overactivation phosphorylated Transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1 a. k. MAP3K7) during development pancreatic cancer in Human and mouse PDAC models. demonstrated that phospho-TAK1 activity was increased human TMA tissues, it’s correlated high...
Lipid rafts are dynamic, nanoscale assemblies of specific proteins and lipids, distributed heterogeneously on eukaryotic membrane. Flotillin-1, a conserved raft marker protein (RMP) harbor SPFH (Stomatin, Prohibitin, Flotillin, HflK/C) oligomerization domains to regulate various cellular processes through its interactions with other signaling or transport proteins. Rafts were thought be absent in prokaryotes hitherto, but recent report presence significance physiology Bacillus subtilis...
Group A Streptococcus (GAS) infection causes a range of diseases, but vaccine development is hampered by the high number serotypes. Here, using reverse vaccinology authors identify SPy_2191 as cross-protective candidate. From 18 initially identified surface proteins, only conserved, surface-exposed and inhibits both GAS adhesion invasion. immunization in mice generates bactericidal antibodies resulting opsonophagocytic killing prevalent invasive serotypes different geographical regions,...
Currently used Brucella vaccines, abortus strain 19 and RB51, comprises of live attenuated strains prevent infection in animals. However, these vaccines pose potential risks to recipient animals such as attenuation reversal virulence susceptible hosts on administration. In this context, recombinant subunit emerge a safe competent alternative combating the disease. study, we formulated divalent vaccine consisting Omp25 L7/L12 B. evaluated individually well combination. Sera obtained from...
Anthrax infection is primarily initiated by B. anthracis endospores that on entry into the host germinate to vegetative cells and cause severe bacteremia toxaemia employing an array of colonisation factors lethal tripartite toxin. The protective efficacy conventional antigen (PA) based anthrax vaccines improved co-administration with inactivated spores or its components. In present study, using structural vaccinology rationale we synthesized a bivalent protein r-PB encompassing toxin (PAIV)...
Thermophilic bacilli in both aerobic or facultative anaerobic forms have been isolated for over a hundred years from different mesophilic thermophilic environments as they are potential source of bioactive secondary metabolites. But the taxonomic resolution Bacillus genus at species strain level is very challenging insufficient divergence 16S rRNA genes. One such recurring problem among anthracis, B. cereus and thuringiensis. The disease-causing anthracis strains their characteristic...
Anthrax vaccines primarily relying only on protective antigen (PA), the cell binding component in anthrax toxins provide incomplete protection when challenged with spores of virulent encapsulated Bacillus anthracis strains. Alternatively, formaldehyde inactivated (FIS) or recombinant spore components generate anti-spore immune responses that inhibit early stages infection and augment PA efficacy. In present study domain IV was spliced exosporium BxpB via a flexible G4S linker to single...
Pulmonary Alveolar Proteinosis (PAP) is a rare condition with an incidence of one in two million and classified as primary or secondary. This the first reported case presenting slow resolving pneumonia.
<h3>Background</h3> Infiltration of myeloid-derived suppressor cells (MDSCs) establishes an immune-suppressive tumor microenvironment (TME) in Pancreatic Ductal Adenocarcinoma (PDAC), exacerbated by chronic inflammation and recurrent gemcitabine (GEM) mediated chemotherapy. Consequently, developing immune-modulatory pro-drug form GEM, targeting modulating the suppressive TME & TAMs, holds promise for preventing cancer recurrence increasing survival rates. <h3>Methods</h3> To develop...
Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and treatment-refractory malignancies. The lack an effective screening tool results in majority patients being diagnosed at late stages, which underscores urgent need to develop more sensitive specific imaging modalities, particularly detecting occult metastases, aid clinical decision-making. tumor microenvironment PDAC heavily infiltrated with myeloid-derived suppressor cells (MDSCs) that express C–C chemokine receptor...
Abstract Background Bacillus anthracis (BA), the etiological agent of anthrax, secretes protective antigen (PA), lethal factor (LF), and edema (EF) as major virulence factors. Among them, PA based vaccines are most indispensable for providing immunity against BA, but low shelf life limits its reliability. Previous studies revealed that domain IV includes B-cell epitopes designated ID I, II, III; among II III have been found to possess more toxin neutralization activity produce high antibody...
Abstract Effective immunotherapy in pancreatic ductal adenocarcinoma (PDAC) is impeded by multiple barriers the tumor microenvironment. These include dense extracellular matrix (ECM), excessive inhibitory myeloid cells, cytokines and chemokines, which collectively incapacitate anti-tumour T cells. Constitutive activation NF-kB pathway a mechanism that drives intrinsic survival of PDAC cells stromal fibrosis, but its impact on immunity has not been investigated. Using The Cancer Genome Atlas...