Combinatorial methods of chemical synthesis allow the creation molecular libraries having immense diversity. The utility such is dependent upon identifying structures molecules so prepared. We describe construction a peptide combinatorial library, 117,649 different members, synthesized on beads and indexed with inert tags. These tags are used as binary code to record reaction history each bead. can be read directly from single bead by electron capture capillary gas chromatography....

The mouse Chromosome 1 locus Ity regulates the extent to which Salmonella typhimurium replicates within reticuloendothelial cell system (RES) during first days of infection. If animals are homozygous for Itys susceptibility allele, Gram-negative bacterium undergoes rapid net multiplication, and mice die a typhoid fever-like disease by day 10 Animals that or heterozygous resistance Ityr, control bacterial replication survive phase salmonellosis. Indirect studies have implicated resident...

CR Climate Research Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 14:219-233 (2000) - doi:10.3354/cr014219 The potential impacts of climate change on mid-Atlantic coastal region Raymond G. Najjar1,*, Henry A. Walker2, Patti J. Anderson3, Eric Barron4, Richard Bord5, Jody R. Gibson6, Victor S. Kennedy7, C. Gregory Knight8, Patrick Megonigal9, Robert E. O¹Connor10, Colin D. Polsky8, Norbert P....

The in vitro and vivo antibacterial activity of A-56268 (TE-031), the 6-O-methyl derivative erythromycin, was compared with those erythromycin other reference drugs. had same spectrum as erythromycin. generally 1 log2 dilution more potent or equal to against all organisms except haemophilus influenzae Propionibacterium acnes, for which 3 dilutions, respectively, less potent. MBC not significantly different from MIC Streptococcus pyogenes, pneumoniae, Staphylococcus epidermidis, H. but than 2...

There is no effective therapy to treat Mycobacterium avium complex infection in patients with acquired immune deficiency syndrome. Clarithromycin (A-56268; TE-031) a new macrolide which twofold more active than erythromycin against most aerobic bacteria. In addition, higher levels serum and tissue are achieved clarithromycin erythromycin. this study, clarithromycin, erythromycin, difloxacin, temafloxacin, ciprofloxacin, rifampin, amikacin, ethambutol were tested vitro vivo the M. complex....

Background. (1,3)-β-D-glucan (BG) is a biomarker for invasive candidiasis (IC). The usefulness of BG level as prognostic marker treatment outcome not well characterized.

The fidelity of protein biosynthesis in any cell rests on the accuracy aminoacylation tRNA. exquisite specificity this reaction is critically dependent correct recognition tRNA by aminoacyl-tRNA synthetases. It shown here that relative concentrations a and its cognate synthetase are normally well balanced crucial for maintenance accurate aminoacylation. When Escherichia coli Gln-tRNA overproduced vivo, it incorrectly acylates supF amber suppressor Tyr with Gln. This effect abolished when...

MICs of clarithromycin and its major human metabolite, 14-hydroxy-clarithromycin, for Haemophilus influenzae in combination were reduced two- to fourfold compared with the each compound alone. Serum parent metabolite medium without serum. In serum spiked clinically relevant concentrations 14-hydroxy-clarithromycin at a fixed ratio 4:1, 15 16 strains (94%) inhibited killed by combinations containing 1.2 0.3 micrograms/ml, respectively. time kill experiments, one-fourth one-half their...

Ecclesia Anglicana clerics and careers the Church political order two laws, one Kingdom economic activity mirrors on men's souls - orthodox spirituality nonconformity dislocation.

The in vitro and vivo properties of a new 1-difluorophenyl-6-fluoroquinolone, temafloxacin hydrochloride (A-62254), were compared with those difloxacin ciprofloxacin. Temafloxacin was as active ciprofloxacin against staphylococci 2 twofold dilutions more than streptococci. Against gram-negative enteric bacteria Pseudomonas aeruginosa, but to 4 less MICs increased by 5 urine at pH 6.5 twofold-dilution increases the hydrochloride, difloxacin, 1 3 serum. same or within pHs 6.5, 7.2, 8.0. When...

Abstract Background Hospitalized patients are at increased risk for candidemia and invasive candidiasis (C/IC). Improved therapeutic regimens with enhanced clinical pharmacoeconomic outcomes utilizing existing antifungal agents still needed. Methods An open-label, non-comparative study evaluated an intravenous (IV) to oral step-down strategy. Patients C/IC were treated IV anidulafungin after 5 days of therapy had the option azole (fluconazole or voriconazole) if they met prespecified...

A single-site mutant of Escherichia coli glutaminyl-synthetase (D235N, GlnRS7) that incorrectly acylates in vivo the amber suppressor supF tyrosine transfer RNA (tRNA(Tyr] with glutamine has been described. Two additional forms enzyme showing this misacylation property have now isolated (D235G, GlnRS10; I129T, GlnRS15). All three mischarging enzymes still retain a certain degree tRNA specificity; they acylate supE glutaminyl (tRNA(Gln] and tRNA(Tyr) but not number other tRNA's. These genetic...

Tiacumicins B and C are members of a novel group 18-membered macrolide antibiotics with in vitro activity against Clostridium difficile. The MICs 15 strains C. difficile were 0.12 to 0.25 microgram/ml for tiacumicin B, 1 C, 0.5 vancomycin. resistance frequency both compounds was less than 2.8 x 10(-8) at four eight times the MIC. vivo activities tiacumicins two compared that vancomycin hamster model antibiotic-associated colitis. Oral therapy 0.2, 1, or 5 mg per kg body weight protected 100%...

While screening for genes that affect the synthesis of yeast snRNPs, we Identified a thermosensitive mutant abolishes production reporter snRNA at non-permissive temperature. This defines new gene, named BDF1. In bdf1-1 strain, synthesized before temperature shift remains stable demonstrates BDF1 gene affects rather than stability and suggests encodes transcription factor. Is present in single copy on chromosome XII, important normal vegetative growth but not essential cell viability. bdf1...

We describe the genetically engineered overproduction of Escherichia coli tRNAGln2, its purification by high pressure liquid chromatography (HPLC), and subsequent use in growth crystals E. glutaminyl-tRNA synthetase-tRNAGln complex. The overproduced tRNA represents 60 to 70% total extracted from strain. A single anion exchange HPLC column is then sufficient increase purity this isoacceptor 90 95%. Crystals material complexed with monomeric synthetase enzyme were obtained vapor diffusion...

Abstract The early response of inbred mice to infection with S. typhimurium is controlled by the mouse Chromosome 1 locus, Ity. To better understand expression this gene, initial interactions between reticuloendothelial system (RES) and i.v. injected salmonellae were compared in resistant (Ityr) susceptible (Itys) mice. In both strains 99% bacteria was cleared from blood within 2 hr, uptake splenic hepatic macrophages similar regardless Ity genotype. vivo phagocytosis followed a 30 60%...

With their solid physical, social and economic presences, strong aura of institutional identity, individual medieval religious houses tend to be treated by historians as distinctly autonomous bodies. That they had (or were meant have) patrons, enjoying ill-defined rights over the responsibilities them, is acknowledged from time time, but rarely considered a significant issue in historiography. Patrons are certainly recognised important early stages house's existence; but, once it up running,...

Transcription factor TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. The from Saccharomyces cerevisiae, also called tau, is a large, multisubunit protein (550-650 kDa) containing two polypeptides that interact directly with DNA encoding (tDNA). We have obtained peptide sequences the 95-kDa DNA-binding subunit (tau 95) cloned corresponding gene, TFC1. encodes polypeptide of calculated Mr 73,500. However, when TFC1 was transcribed translated in...

Journal Article Antipneumococcal Activity of Ciprofloxacin, Ofloxacin, and Temafloxacin in an Experimental Mouse Pneumonia Model at Various Stages the Disease Get access E. Azoulay-Dupuis, Azoulay-Dupuis Institut de la Santé et Recherche Médicale, Hôpital Claude Bernard, Paris, France; Anti-Infective Research, Abbott Laboratories, Park, Illinois Reprints or correspondence: Dr. INSERM UB, Hopital 10, Avenue Porte d'Aubervilliers, 75944 France. Search for other works by this author on: Oxford...